Literature summary for 1.1.1.25 extracted from

Fonseca, I.O.; Magalhaes, M.L.; Oliveira, J.S.; Silva, R.G.; Mendes, M.A.; Palma, M.S.; Santos, D.S.; Basso, L.A.
Functional shikimate dehydrogenase from Mycobacterium tuberculosis H37Rv: purification and characterization (2006), Protein Expr. Purif., 46, 429-437.

Search for more literature for 1.1.1.25

Cloned(Commentary)

Cloned (Comment)	Organism
gene aroE, DNA and amino acid sequence determination, functional expression as soluble enzyme in Escherichia coli strain BL21(DE3)	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetics of recombinant AroE	Mycobacterium tuberculosis
0.01	-	NADPH	pH 7.0, 25°C, recombinant AroE	Mycobacterium tuberculosis
0.022	-	NADP+	pH 7.0, 25°C, recombinant AroE	Mycobacterium tuberculosis
0.031	-	3-dehydroshikimate	pH 7.0, 25°C, recombinant AroE	Mycobacterium tuberculosis
0.05	-	shikimate	pH 7.0, 25°C, recombinant AroE	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	1 * 27000, recombinant AroE, SDS-PAGE, 1 * 27076, recombinant AroE, mass spectrometry	Mycobacterium tuberculosis
27076	-	1 * 27000, recombinant AroE, SDS-PAGE, 1 * 27076, recombinant AroE, mass spectrometry	Mycobacterium tuberculosis
27080	-	recombinant AroE, mass spectrometry	Mycobacterium tuberculosis
54150	-	recombinant AroE, mass spectrometry	Mycobacterium tuberculosis
58370	-	recombinant AroE, gel filtration	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	A5U5Q2	gene aroE	-
Mycobacterium tuberculosis H37Rv	A5U5Q2	gene aroE	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble enzyme 8.5fold from Escherichia coli strain BL21(DE3) by ion exchange and hydrophobic interaction chromatography, gel filtration, and again ion exchange chromatography, to homogeneity	Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.9	-	purified recombinant AroE	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
shikimate + NADP+	-	Mycobacterium tuberculosis	3-dehydroshikimate + NADPH + H+	-	?
shikimate + NADP+	-	Mycobacterium tuberculosis H37Rv	3-dehydroshikimate + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 54150, recombinant AroE, mass spectrometry	Mycobacterium tuberculosis
monomer	1 * 27000, recombinant AroE, SDS-PAGE, 1 * 27076, recombinant AroE, mass spectrometry	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
AroE	-	Mycobacterium tuberculosis
SD	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	purified recombinant AroE, stable for at least 1 h	Mycobacterium tuberculosis
55	-	purified recombinant AroE, 20% remaining activity after 1 h	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.9	-	NADP+	pH 7.0, 25°C, recombinant AroE	Mycobacterium tuberculosis
8.2	-	shikimate	pH 7.0, 25°C, recombinant AroE	Mycobacterium tuberculosis
45	-	NADPH	pH 7.0, 25°C, recombinant AroE	Mycobacterium tuberculosis
49	-	3-dehydroshikimate	pH 7.0, 25°C, recombinant AroE	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)