Cloned (Comment) | Organism |
---|---|
phylogenetic analysis of the three paralogues YdiB, AroE, and HI0607, DNA and amino acid sequence determination and analysis of paralogue HI0607, expression of wild-type paralogue HI0607 in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in strain B834(DE3) | Haemophilus influenzae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-labeled paralogue HI0607, hanging drop vapour diffusion method, 0.001 ml protein solution containing 20 mg/ml protein mixed with equal volume of reservoir solution containing 0.1 M sodium acetate, pH 4.6, 2.0 M NaCl, and 10% v/v PEG 400, X-ray diffraction structure determination and analysis at 1.75 A resolution | Haemophilus influenzae |
Protein Variants | Comment | Organism |
---|---|---|
D103X | site-directed mutagenesis of paralogue HI0607, inactive mutant | Haemophilus influenzae |
K67H | site-directed mutagenesis of paralogue HI0607, inactive mutant | Haemophilus influenzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition of paralogue HI0607 by EDTA | Haemophilus influenzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | paralogue HI0607 is not affected by 5 mM of Zn2+, Ca2+, Mg2+, or Mn2+ | Haemophilus influenzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinate + NADP+ | Haemophilus influenzae | reversible activity of YdiB, no activity with paralogue HI0607 | 3-dehydroquinate + NADPH + H+ | - |
r | |
shikimate + NADP+ | Haemophilus influenzae | reversible activity of YdiB and AroE, paralogue HI0607 catalyzes the oxidative reaction with low activity | 3-dehydroshikimate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
three paralogues: YdiB, AroE, and the shikimate dehydrogenase-like HI0607 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+ | conserved residues Asp103 and Lys67 are important for catalysis in all three paralogues, kinetic mechanism of paralogue HI0607 and substrate binding site | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinate + NADP+ | reversible activity of YdiB, no activity with paralogue HI0607 | Haemophilus influenzae | 3-dehydroquinate + NADPH + H+ | - |
r | |
shikimate + NADP+ | reversible activity of YdiB and AroE, paralogue HI0607 catalyzes the oxidative reaction with low activity | Haemophilus influenzae | 3-dehydroshikimate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | recombinant paralogue HI0607, SDS-PAGE and gel filtration | Haemophilus influenzae |
Synonyms | Comment | Organism |
---|---|---|
AroE | - |
Haemophilus influenzae |
HI0607 | - |
Haemophilus influenzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Haemophilus influenzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.8 | paralogue HI0607 | Haemophilus influenzae |
8.5 | - |
assay at | Haemophilus influenzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile, paralogue HI0607 | Haemophilus influenzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | cofactor binding site of paralogue HI0607 | Haemophilus influenzae | |
NADPH | - |
Haemophilus influenzae |