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show all sequences of 1.1.1.248

Purification and characterization of salutaridine:NADPH 7-oxidoreductase from Papaver somniferum

Gerardy, R.; Zenk, M.H.; Phytochemistry 34, 125-132 (1993)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
salutaridinol
-
Papaver somniferum
0.023
-
salutaridine
-
Papaver somniferum
0.055
-
NADP+
-
Papaver somniferum
0.125
-
NADPH
-
Papaver somniferum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Papaver somniferum
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
gel filtration
Papaver somniferum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
salutaridine + NADPH + H+
Papaver somniferum
formation of salutaridinol, a precursor for the synthesis of morphine and related opium alkaloids
salutaridinol + NADP+
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
no activity in Papaver alpinum
-
-
-
no activity in Papaver atlanticum
-
-
-
no activity in Papaver dubium
-
-
-
no activity in Papaver feddei
-
-
-
no activity in Papaver lateritium
-
-
-
no activity in Papaver oreophilum
-
-
-
no activity in Papaver orientale
-
-
-
no activity in Papaver persicum
-
-
-
no activity in Papaver pilosum
-
-
-
no activity in Papaver rhoeas
-
-
-
no activity in Papaver rupifragum
-
-
-
no activity in Papaver strigosum
-
-
-
no activity in Papaver tauricula
-
-
-
no activity in Papaver triniifolium
-
-
-
Papaver bracteatum
-
-
-
Papaver somniferum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Papaver somniferum
Reaction
Reaction
Commentary
Organism
salutaridinol + NADP+ = salutaridine + NADPH + H+
highly substrate specific transfer of the pro-S hydride, B-type, of NADPH to C-7 of salutaridine
Papaver somniferum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Papaver somniferum
-
additional information
no activity in leaves
Papaver somniferum
-
plant capsule
-
Papaver somniferum
-
root
-
Papaver somniferum
-
seedling
-
Papaver somniferum
-
shoot
-
Papaver somniferum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Papaver somniferum
Storage Stability
Storage Stability
Organism
4°C, pH 7.0-9.0, 0.6 M KCl, half-life: 30 days
Papaver somniferum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
salutaridine + NADPH + H+
r
9554
Papaver somniferum
(7S)-salutaridinol + NADP+
-
9554
Papaver somniferum
-
salutaridine + NADPH + H+
formation of salutaridinol, a precursor for the synthesis of morphine and related opium alkaloids
9554
Papaver somniferum
salutaridinol + NADP+
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 52000, SDS-PAGE
Papaver somniferum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
35
both directions
Papaver somniferum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
formation of (7S)-salutaridinol
Papaver somniferum
9
9.5
formation of salutaridine
Papaver somniferum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.8
7.8
50% of maximal activity at pH 5.8 and at pH 7.8, formation of (7S)-salutaridinol
Papaver somniferum
8.5
10
50% of maximal activity at pH 5.8 and at pH 7.8, formationof salutaridine
Papaver somniferum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
9
4°C, half-life: 30 days
Papaver somniferum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
salutaridinol
-
Papaver somniferum
0.023
-
salutaridine
-
Papaver somniferum
0.055
-
NADP+
-
Papaver somniferum
0.125
-
NADPH
-
Papaver somniferum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Papaver somniferum
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
gel filtration
Papaver somniferum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
salutaridine + NADPH + H+
Papaver somniferum
formation of salutaridinol, a precursor for the synthesis of morphine and related opium alkaloids
salutaridinol + NADP+
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Papaver somniferum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Papaver somniferum
-
additional information
no activity in leaves
Papaver somniferum
-
plant capsule
-
Papaver somniferum
-
root
-
Papaver somniferum
-
seedling
-
Papaver somniferum
-
shoot
-
Papaver somniferum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Papaver somniferum
Storage Stability (protein specific)
Storage Stability
Organism
4°C, pH 7.0-9.0, 0.6 M KCl, half-life: 30 days
Papaver somniferum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
salutaridine + NADPH + H+
r
9554
Papaver somniferum
(7S)-salutaridinol + NADP+
-
9554
Papaver somniferum
-
salutaridine + NADPH + H+
formation of salutaridinol, a precursor for the synthesis of morphine and related opium alkaloids
9554
Papaver somniferum
salutaridinol + NADP+
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 52000, SDS-PAGE
Papaver somniferum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
35
both directions
Papaver somniferum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
formation of (7S)-salutaridinol
Papaver somniferum
9
9.5
formation of salutaridine
Papaver somniferum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.8
7.8
50% of maximal activity at pH 5.8 and at pH 7.8, formation of (7S)-salutaridinol
Papaver somniferum
8.5
10
50% of maximal activity at pH 5.8 and at pH 7.8, formationof salutaridine
Papaver somniferum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
9
4°C, half-life: 30 days
Papaver somniferum
Other publictions for EC 1.1.1.248
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720707
Wijekoon
Systematic knockdown of morphi ...
Papaver somniferum
Plant J.
69
1052-1063
2012
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
725460
Higashi
Atomic structure of salutaridi ...
Papaver somniferum
J. Biol. Chem.
286
6532-6541
2011
-
-
1
1
15
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
15
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
710745
Higashi
Crystallization and preliminar ...
Papaver somniferum
Acta Crystallogr. Sect. F
66
163-166
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698937
Ziegler
Removal of substrate inhibitio ...
Papaver somniferum
J. Biol. Chem.
284
26758-26767
2009
-
-
1
-
12
-
2
26
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
13
-
-
-
1
1
-
-
-
-
1
1
-
12
-
-
2
1
26
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
700676
Ziegler
Evolution of morphine biosynth ...
Papaver arenarium, Papaver bracteatum, Papaver pilosum, Papaver pyrenaicum, Papaver somniferum
Phytochemistry
70
1696-1707
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700683
Kempe
RNAi suppression of the morphi ...
Papaver somniferum
Phytochemistry
70
579-589
2009
-
-
1
-
-
-
1
-
1
-
-
-
-
3
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689571
Geissler
Molecular modeling and site-di ...
Papaver bracteatum
Plant Physiol.
143
1493-1503
2007
-
-
1
1
13
-
1
6
-
-
2
2
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
5
2
1
-
4
1
1
-
-
-
1
4
1
13
-
-
1
1
6
-
-
2
2
-
-
-
1
-
-
-
-
4
1
1
-
-
5
2
1
-
1
-
-
-
-
-
-
670562
Ziegler
Comparative transcript and alk ...
Papaver somniferum, Papaver somniferum salR
Plant J.
48
177-192
2006
-
-
1
-
-
-
-
4
-
-
3
4
-
6
-
-
1
-
-
-
-
-
8
1
1
1
-
-
2
2
-
4
-
1
-
-
-
1
4
-
-
-
-
-
-
4
-
-
3
4
-
-
-
1
-
-
-
-
8
1
1
1
-
-
2
2
-
1
-
-
-
-
-
-
9554
Gerardy
-
Purification and characterizat ...
no activity in Papaver alpinum, no activity in Papaver atlanticum, no activity in Papaver dubium, no activity in Papaver feddei, no activity in Papaver lateritium, no activity in Papaver oreophilum, no activity in Papaver orientale, no activity in Papaver persicum, no activity in Papaver pilosum, no activity in Papaver rhoeas, no activity in Papaver rupifragum, no activity in Papaver strigosum, no activity in Papaver tauricula, no activity in Papaver triniifolium, Papaver bracteatum, Papaver somniferum
Phytochemistry
34
125-132
1993
-
-
-
-
-
-
-
4
1
-
1
1
-
16
-
-
1
1
-
6
1
1
2
1
1
-
-
-
2
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
1
1
-
-
-
1
-
6
1
1
2
1
1
-
-
-
2
2
1
-
-
-
-
-
-
-