BRENDA - Enzyme Database show
show all sequences of 1.1.1.248

RNAi suppression of the morphine biosynthetic gene salAT and evidence of association of pathway enzymes

Kempe, K.; Higashi, Y.; Frick, S.; Sabarna, K.; Kutchan, T.M.; Phytochemistry 70, 579-589 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloned into the pMyr vector and expressed as fusion protein with a myristylation sequence, which anchors the target protein to the yeast membrane. Fusion proteins (pSOS/SalAT and pMyr/SalR) coexpressed in the Saccharomyces cerevisiae cdc25H strain. SalR amplified from the recombinant plasmid SalR/pQE-30. The 0.9-kb DNA fragment ligated into an NheI/EcoRI-digested pET28a expression vector. SalR/pET28a expression construct transformed into Escherichia coli BL21(DE3)RIL
Papaver somniferum
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
direct influence of a reduction in salAT transcript level by RNAi on the transcription of salR is not evident
Papaver somniferum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Papaver somniferum
5829
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Papaver somniferum
-
-
-
Purification (Commentary)
Commentary
Organism
His-tagged SalR purified with a cobalt affinity resin
Papaver somniferum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Papaver somniferum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
salutaridine + NADPH + H+
interaction between SalR and SalAT in morphine biosynthesis
700683
Papaver somniferum
(7S)-salutaridinol + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Papaver somniferum
Cloned(Commentary) (protein specific)
Commentary
Organism
cloned into the pMyr vector and expressed as fusion protein with a myristylation sequence, which anchors the target protein to the yeast membrane. Fusion proteins (pSOS/SalAT and pMyr/SalR) coexpressed in the Saccharomyces cerevisiae cdc25H strain. SalR amplified from the recombinant plasmid SalR/pQE-30. The 0.9-kb DNA fragment ligated into an NheI/EcoRI-digested pET28a expression vector. SalR/pET28a expression construct transformed into Escherichia coli BL21(DE3)RIL
Papaver somniferum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Papaver somniferum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
direct influence of a reduction in salAT transcript level by RNAi on the transcription of salR is not evident
Papaver somniferum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Papaver somniferum
5829
-
Purification (Commentary) (protein specific)
Commentary
Organism
His-tagged SalR purified with a cobalt affinity resin
Papaver somniferum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Papaver somniferum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
salutaridine + NADPH + H+
interaction between SalR and SalAT in morphine biosynthesis
700683
Papaver somniferum
(7S)-salutaridinol + NADP+
-
-
-
?
Other publictions for EC 1.1.1.248
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720707
Wijekoon
Systematic knockdown of morphi ...
Papaver somniferum
Plant J.
69
1052-1063
2012
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-
1
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1
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1
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2
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1
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2
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1
2
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1
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1
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1
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2
2
-
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725460
Higashi
Atomic structure of salutaridi ...
Papaver somniferum
J. Biol. Chem.
286
6532-6541
2011
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1
1
15
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1
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3
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1
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1
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2
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1
2
1
15
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1
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1
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1
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3
3
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710745
Higashi
Crystallization and preliminar ...
Papaver somniferum
Acta Crystallogr. Sect. F
66
163-166
2010
-
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1
1
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4
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1
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1
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1
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1
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2
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698937
Ziegler
Removal of substrate inhibitio ...
Papaver somniferum
J. Biol. Chem.
284
26758-26767
2009
-
-
1
-
12
-
2
26
-
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1
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1
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1
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13
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1
1
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1
1
-
12
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2
1
26
-
-
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-
-
1
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-
-
-
1
-
-
-
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13
-
-
-
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-
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-
-
700676
Ziegler
Evolution of morphine biosynth ...
Papaver arenarium, Papaver bracteatum, Papaver pilosum, Papaver pyrenaicum, Papaver somniferum
Phytochemistry
70
1696-1707
2009
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1
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8
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5
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5
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1
5
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5
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-
700683
Kempe
RNAi suppression of the morphi ...
Papaver somniferum
Phytochemistry
70
579-589
2009
-
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1
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1
-
1
-
-
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3
-
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
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689571
Geissler
Molecular modeling and site-di ...
Papaver bracteatum
Plant Physiol.
143
1493-1503
2007
-
-
1
1
13
-
1
6
-
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2
2
-
4
-
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1
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4
1
1
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5
2
1
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4
1
1
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1
4
1
13
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1
1
6
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2
2
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1
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4
1
1
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5
2
1
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1
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670562
Ziegler
Comparative transcript and alk ...
Papaver somniferum, Papaver somniferum salR
Plant J.
48
177-192
2006
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1
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4
-
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3
4
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6
-
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1
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8
1
1
1
-
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2
2
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4
-
1
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1
4
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4
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3
4
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1
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8
1
1
1
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2
2
-
1
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-
9554
Gerardy
-
Purification and characterizat ...
no activity in Papaver alpinum, no activity in Papaver atlanticum, no activity in Papaver dubium, no activity in Papaver feddei, no activity in Papaver lateritium, no activity in Papaver oreophilum, no activity in Papaver orientale, no activity in Papaver persicum, no activity in Papaver pilosum, no activity in Papaver rhoeas, no activity in Papaver rupifragum, no activity in Papaver strigosum, no activity in Papaver tauricula, no activity in Papaver triniifolium, Papaver bracteatum, Papaver somniferum
Phytochemistry
34
125-132
1993
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-
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4
1
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1
1
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16
-
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1
1
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6
1
1
2
1
1
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2
2
1
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4
1
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1
1
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1
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6
1
1
2
1
1
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2
2
1
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