BRENDA - Enzyme Database show
show all sequences of 1.1.1.248

Comparative transcript and alkaloid profiling in Papaver species identifies a short chain dehydrogenase/reductase involved in morphine biosynthesis

Ziegler, J.; Voigtlaender, S.; Schmidt, J.; Kramell, R.; Miersch, O.; Ammer, C.; Gesell, A.; Kutchan, T.M.; Plant J. 48, 177-192 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene salR, DNA and amino acid sequence determination and analysis, expression profile, overexpression in Escherichia coli strain SG13009 as His6-tagged enzyme
Papaver somniferum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics, recombinant enzyme
Papaver somniferum
0.031
-
salutaridine
pH 6.0, 30°C, recombinant enzyme, reduction reaction
Papaver somniferum
0.08
-
NADPH
pH 6.0, 30°C, recombinant enzyme, reduction reaction
Papaver somniferum
0.198
-
NADP+
pH 9.5, 30°C, recombinant enzyme, oxidation reaction
Papaver somniferum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34100
-
1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE
Papaver somniferum
40000
-
1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE
Papaver somniferum
47100
50100
recombinant enzyme, gel filtration
Papaver somniferum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Papaver somniferum
alkaloid composition of the organism
?
-
-
-
additional information
Papaver somniferum salR
alkaloid composition of the organism
?
-
-
-
salutaridine + NADPH + H+
Papaver somniferum
a step in the unique biosynthesis of morphine via codeine, overview
(7S)-salutaridinol + NADP+
-
-
r
salutaridine + NADPH + H+
Papaver somniferum salR
a step in the unique biosynthesis of morphine via codeine, overview
(7S)-salutaridinol + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Papaver somniferum
Q071N0
gene salR; salR
-
Papaver somniferum salR
Q071N0
gene salR; salR
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain SG13009
Papaver somniferum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
alkaloid composition of the organism
670562
Papaver somniferum
?
-
-
-
-
additional information
no reduction of 1,2-nordehydroreticuline, 1,2-dehydroreticulinium ion, codeinone, tropinone, or (-)-menthone, no oxidation of (R)-norreticuline, (R)-reticuline, codeine, tropine, or (+)-neomenthol
670562
Papaver somniferum
?
-
-
-
-
additional information
alkaloid composition of the organism
670562
Papaver somniferum salR
?
-
-
-
-
additional information
no reduction of 1,2-nordehydroreticuline, 1,2-dehydroreticulinium ion, codeinone, tropinone, or (-)-menthone, no oxidation of (R)-norreticuline, (R)-reticuline, codeine, tropine, or (+)-neomenthol
670562
Papaver somniferum salR
?
-
-
-
-
salutaridine + NADPH + H+
a step in the unique biosynthesis of morphine via codeine, overview
670562
Papaver somniferum
(7S)-salutaridinol + NADP+
-
-
-
r
salutaridine + NADPH + H+
NADPH-dependent reduction of the 7-keto group
670562
Papaver somniferum
(7S)-salutaridinol + NADP+
no formation of 7-epi-salutaridinol
-
-
r
salutaridine + NADPH + H+
a step in the unique biosynthesis of morphine via codeine, overview
670562
Papaver somniferum salR
(7S)-salutaridinol + NADP+
-
-
-
r
salutaridine + NADPH + H+
NADPH-dependent reduction of the 7-keto group
670562
Papaver somniferum salR
(7S)-salutaridinol + NADP+
no formation of 7-epi-salutaridinol
-
-
r
Subunits
Subunits
Commentary
Organism
monomer
1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE
Papaver somniferum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
35
recombinant enzyme, reduction reaction
Papaver somniferum
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
40
recombinant enzyme, 50% of maximal activity at 20°C and 40°C, reduction reaction
Papaver somniferum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
recombinant enzyme, reduction reaction
Papaver somniferum
9.5
-
oxidation reaction, recombinant enzyme
Papaver somniferum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.7
7.5
sharp increase in activity at pH 4.7, half-maximal activity at pH 7.5, inactive at pH 9.0, recombinant enzyme, reduction reaction
Papaver somniferum
9
10
maximal activity at pH 9.5, sharp decline in activity at pH 10.0, oxidation reaction, recombinant enzyme
Papaver somniferum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
5% of the activity with NADP+, oxidation reaction, recombinant enzyme
Papaver somniferum
NADH
20% of the activity with NADPH, reduction reaction, recombinant enzyme
Papaver somniferum
NADP+
preferred cofactor, oxidation reaction, recombinant enzyme
Papaver somniferum
NADPH
preferred cofactor, reduction reaction, recombinant enzyme
Papaver somniferum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Papaver somniferum
amino acid sequence calculation
-
4.7
Cloned(Commentary) (protein specific)
Commentary
Organism
gene salR, DNA and amino acid sequence determination and analysis, expression profile, overexpression in Escherichia coli strain SG13009 as His6-tagged enzyme
Papaver somniferum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
5% of the activity with NADP+, oxidation reaction, recombinant enzyme
Papaver somniferum
NADH
20% of the activity with NADPH, reduction reaction, recombinant enzyme
Papaver somniferum
NADP+
preferred cofactor, oxidation reaction, recombinant enzyme
Papaver somniferum
NADPH
preferred cofactor, reduction reaction, recombinant enzyme
Papaver somniferum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics, recombinant enzyme
Papaver somniferum
0.031
-
salutaridine
pH 6.0, 30°C, recombinant enzyme, reduction reaction
Papaver somniferum
0.08
-
NADPH
pH 6.0, 30°C, recombinant enzyme, reduction reaction
Papaver somniferum
0.198
-
NADP+
pH 9.5, 30°C, recombinant enzyme, oxidation reaction
Papaver somniferum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34100
-
1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE
Papaver somniferum
40000
-
1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE
Papaver somniferum
47100
50100
recombinant enzyme, gel filtration
Papaver somniferum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Papaver somniferum
alkaloid composition of the organism
?
-
-
-
additional information
Papaver somniferum salR
alkaloid composition of the organism
?
-
-
-
salutaridine + NADPH + H+
Papaver somniferum
a step in the unique biosynthesis of morphine via codeine, overview
(7S)-salutaridinol + NADP+
-
-
r
salutaridine + NADPH + H+
Papaver somniferum salR
a step in the unique biosynthesis of morphine via codeine, overview
(7S)-salutaridinol + NADP+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain SG13009
Papaver somniferum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
alkaloid composition of the organism
670562
Papaver somniferum
?
-
-
-
-
additional information
no reduction of 1,2-nordehydroreticuline, 1,2-dehydroreticulinium ion, codeinone, tropinone, or (-)-menthone, no oxidation of (R)-norreticuline, (R)-reticuline, codeine, tropine, or (+)-neomenthol
670562
Papaver somniferum
?
-
-
-
-
additional information
alkaloid composition of the organism
670562
Papaver somniferum salR
?
-
-
-
-
additional information
no reduction of 1,2-nordehydroreticuline, 1,2-dehydroreticulinium ion, codeinone, tropinone, or (-)-menthone, no oxidation of (R)-norreticuline, (R)-reticuline, codeine, tropine, or (+)-neomenthol
670562
Papaver somniferum salR
?
-
-
-
-
salutaridine + NADPH + H+
a step in the unique biosynthesis of morphine via codeine, overview
670562
Papaver somniferum
(7S)-salutaridinol + NADP+
-
-
-
r
salutaridine + NADPH + H+
NADPH-dependent reduction of the 7-keto group
670562
Papaver somniferum
(7S)-salutaridinol + NADP+
no formation of 7-epi-salutaridinol
-
-
r
salutaridine + NADPH + H+
a step in the unique biosynthesis of morphine via codeine, overview
670562
Papaver somniferum salR
(7S)-salutaridinol + NADP+
-
-
-
r
salutaridine + NADPH + H+
NADPH-dependent reduction of the 7-keto group
670562
Papaver somniferum salR
(7S)-salutaridinol + NADP+
no formation of 7-epi-salutaridinol
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE
Papaver somniferum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
35
recombinant enzyme, reduction reaction
Papaver somniferum
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
40
recombinant enzyme, 50% of maximal activity at 20°C and 40°C, reduction reaction
Papaver somniferum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
recombinant enzyme, reduction reaction
Papaver somniferum
9.5
-
oxidation reaction, recombinant enzyme
Papaver somniferum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.7
7.5
sharp increase in activity at pH 4.7, half-maximal activity at pH 7.5, inactive at pH 9.0, recombinant enzyme, reduction reaction
Papaver somniferum
9
10
maximal activity at pH 9.5, sharp decline in activity at pH 10.0, oxidation reaction, recombinant enzyme
Papaver somniferum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Papaver somniferum
amino acid sequence calculation
-
4.7
Other publictions for EC 1.1.1.248
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720707
Wijekoon
Systematic knockdown of morphi ...
Papaver somniferum
Plant J.
69
1052-1063
2012
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
725460
Higashi
Atomic structure of salutaridi ...
Papaver somniferum
J. Biol. Chem.
286
6532-6541
2011
-
-
1
1
15
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
15
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
710745
Higashi
Crystallization and preliminar ...
Papaver somniferum
Acta Crystallogr. Sect. F
66
163-166
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698937
Ziegler
Removal of substrate inhibitio ...
Papaver somniferum
J. Biol. Chem.
284
26758-26767
2009
-
-
1
-
12
-
2
26
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
13
-
-
-
1
1
-
-
-
-
1
1
-
12
-
-
2
1
26
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
700676
Ziegler
Evolution of morphine biosynth ...
Papaver arenarium, Papaver bracteatum, Papaver pilosum, Papaver pyrenaicum, Papaver somniferum
Phytochemistry
70
1696-1707
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700683
Kempe
RNAi suppression of the morphi ...
Papaver somniferum
Phytochemistry
70
579-589
2009
-
-
1
-
-
-
1
-
1
-
-
-
-
3
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689571
Geissler
Molecular modeling and site-di ...
Papaver bracteatum
Plant Physiol.
143
1493-1503
2007
-
-
1
1
13
-
1
6
-
-
2
2
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
5
2
1
-
4
1
1
-
-
-
1
4
1
13
-
-
1
1
6
-
-
2
2
-
-
-
1
-
-
-
-
4
1
1
-
-
5
2
1
-
1
-
-
-
-
-
-
670562
Ziegler
Comparative transcript and alk ...
Papaver somniferum, Papaver somniferum salR
Plant J.
48
177-192
2006
-
-
1
-
-
-
-
4
-
-
3
4
-
6
-
-
1
-
-
-
-
-
8
1
1
1
-
-
2
2
-
4
-
1
-
-
-
1
4
-
-
-
-
-
-
4
-
-
3
4
-
-
-
1
-
-
-
-
8
1
1
1
-
-
2
2
-
1
-
-
-
-
-
-
9554
Gerardy
-
Purification and characterizat ...
no activity in Papaver alpinum, no activity in Papaver atlanticum, no activity in Papaver dubium, no activity in Papaver feddei, no activity in Papaver lateritium, no activity in Papaver oreophilum, no activity in Papaver orientale, no activity in Papaver persicum, no activity in Papaver pilosum, no activity in Papaver rhoeas, no activity in Papaver rupifragum, no activity in Papaver strigosum, no activity in Papaver tauricula, no activity in Papaver triniifolium, Papaver bracteatum, Papaver somniferum
Phytochemistry
34
125-132
1993
-
-
-
-
-
-
-
4
1
-
1
1
-
16
-
-
1
1
-
6
1
1
2
1
1
-
-
-
2
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
1
1
-
-
-
1
-
6
1
1
2
1
1
-
-
-
2
2
1
-
-
-
-
-
-
-