BRENDA - Enzyme Database show
show all sequences of 1.1.1.247

Purification and properties of codeinone reductase (NADPH) from Papaver somniferum cell cultures and differentiated plants

Lenz, R.; Zenk, M.H.; Eur. J. Biochem. 233, 132-139 (1995)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.009
-
codeinone
isoenzymes from capsule tissue
Papaver somniferum
0.023
-
codeinone
enzyme from cell culture
Papaver somniferum
0.026
-
Hydrocodone
isoenzymes from capsule tissue
Papaver somniferum
0.027
-
Hydrocodone
enzyme from cell culture; morphine, enzyme from cell culture
Papaver somniferum
0.029
-
NADP+
enzyme from cell culture
Papaver somniferum
0.042
-
Hydromorphone
isoenzymes from capsule tissue
Papaver somniferum
0.047
-
NADP+
isoenzymes from capsule tissue
Papaver somniferum
0.05
-
Hydromorphone
enzyme from cell culture
Papaver somniferum
0.063
-
morphine
isoenzymes from capsule tissue
Papaver somniferum
0.076
-
codeine
enzyme from cell culture
Papaver somniferum
0.081
-
NADPH
isoenzymes from capsule tissue
Papaver somniferum
0.168
-
NADPH
enzyme from cell culture
Papaver somniferum
0.178
-
codeine
isoenzymes from capsule tissue
Papaver somniferum
0.422
-
Dihydrocodeine
isoenzymes from capsule tissue
Papaver somniferum
0.424
-
Dihydrocodeine
enzyme from cell culture
Papaver somniferum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Papaver somniferum
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Papaver somniferum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Codeinone + NADPH
Papaver somniferum
biosynthesis of codeine
Codeine + NADP+
-
-
-
Morphinone + NADPH
Papaver somniferum
biosynthesis of morphine
Morphine + NADP+
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Papaver somniferum
-
-
-
Purification (Commentary)
Commentary
Organism
2 isoenzymes from capsule tissue, enzyme from cell culture
Papaver somniferum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Papaver somniferum
-
leaf
-
Papaver somniferum
-
plant capsule
-
Papaver somniferum
-
root
-
Papaver somniferum
-
shoot
-
Papaver somniferum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Papaver somniferum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Codeinone + NADPH
r
9551
Papaver somniferum
Codeine + NADP+
(-)codeine
9551
Papaver somniferum
-
Codeinone + NADPH
(-)codeinone
9551
Papaver somniferum
Codeine + NADP+
(-)codeine
9551
Papaver somniferum
-
Codeinone + NADPH
no reaction with NADH and NAD+
9551
Papaver somniferum
Codeine + NADP+
(-)codeine
9551
Papaver somniferum
-
Codeinone + NADPH
biosynthesis of codeine
9551
Papaver somniferum
Codeine + NADP+
-
-
-
-
Dihydrocodeine + NADP+
-
9551
Papaver somniferum
?
-
-
-
-
Hydrocodone + NADPH
-
9551
Papaver somniferum
?
-
-
-
-
Hydromorphone + NADPH
-
9551
Papaver somniferum
?
-
-
-
-
Morphinone + NADPH
-
9551
Papaver somniferum
Morphine + NADP+
-
9551
Papaver somniferum
-
Morphinone + NADPH
biosynthesis of morphine
9551
Papaver somniferum
Morphine + NADP+
-
-
-
-
Naloxone + NADPH
-
9551
Papaver somniferum
?
-
-
-
-
Naltrexone + NADPH
-
9551
Papaver somniferum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
34000-35000, SDS-PAGE
Papaver somniferum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
reduction of codeinone
Papaver somniferum
46
-
oxidation of codeine
Papaver somniferum
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
29
47
50% of maximal activity at 29C and 47C, reduction of codeinone
Papaver somniferum
34
53
50% of maximal activity at 34C and 53C, oxidation of codeine
Papaver somniferum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
reduction of codeinone
Papaver somniferum
9.1
-
oxidation of codeine
Papaver somniferum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.2
7.6
50% of maximal activity at pH 6.2 and pH 7.6, reduction of codeinone
Papaver somniferum
8.2
9.8
50% of maximal activity at pH 8.2 and pH 9.8, oxidation of codeine
Papaver somniferum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Papaver somniferum
NADPH
-
Papaver somniferum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Papaver somniferum
NADPH
-
Papaver somniferum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.009
-
codeinone
isoenzymes from capsule tissue
Papaver somniferum
0.023
-
codeinone
enzyme from cell culture
Papaver somniferum
0.026
-
Hydrocodone
isoenzymes from capsule tissue
Papaver somniferum
0.027
-
Hydrocodone
enzyme from cell culture; morphine, enzyme from cell culture
Papaver somniferum
0.029
-
NADP+
enzyme from cell culture
Papaver somniferum
0.042
-
Hydromorphone
isoenzymes from capsule tissue
Papaver somniferum
0.047
-
NADP+
isoenzymes from capsule tissue
Papaver somniferum
0.05
-
Hydromorphone
enzyme from cell culture
Papaver somniferum
0.063
-
morphine
isoenzymes from capsule tissue
Papaver somniferum
0.076
-
codeine
enzyme from cell culture
Papaver somniferum
0.081
-
NADPH
isoenzymes from capsule tissue
Papaver somniferum
0.168
-
NADPH
enzyme from cell culture
Papaver somniferum
0.178
-
codeine
isoenzymes from capsule tissue
Papaver somniferum
0.422
-
Dihydrocodeine
isoenzymes from capsule tissue
Papaver somniferum
0.424
-
Dihydrocodeine
enzyme from cell culture
Papaver somniferum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Papaver somniferum
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Papaver somniferum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Codeinone + NADPH
Papaver somniferum
biosynthesis of codeine
Codeine + NADP+
-
-
-
Morphinone + NADPH
Papaver somniferum
biosynthesis of morphine
Morphine + NADP+
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
2 isoenzymes from capsule tissue, enzyme from cell culture
Papaver somniferum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Papaver somniferum
-
leaf
-
Papaver somniferum
-
plant capsule
-
Papaver somniferum
-
root
-
Papaver somniferum
-
shoot
-
Papaver somniferum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Papaver somniferum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Codeinone + NADPH
r
9551
Papaver somniferum
Codeine + NADP+
(-)codeine
9551
Papaver somniferum
-
Codeinone + NADPH
(-)codeinone
9551
Papaver somniferum
Codeine + NADP+
(-)codeine
9551
Papaver somniferum
-
Codeinone + NADPH
no reaction with NADH and NAD+
9551
Papaver somniferum
Codeine + NADP+
(-)codeine
9551
Papaver somniferum
-
Codeinone + NADPH
biosynthesis of codeine
9551
Papaver somniferum
Codeine + NADP+
-
-
-
-
Dihydrocodeine + NADP+
-
9551
Papaver somniferum
?
-
-
-
-
Hydrocodone + NADPH
-
9551
Papaver somniferum
?
-
-
-
-
Hydromorphone + NADPH
-
9551
Papaver somniferum
?
-
-
-
-
Morphinone + NADPH
-
9551
Papaver somniferum
Morphine + NADP+
-
9551
Papaver somniferum
-
Morphinone + NADPH
biosynthesis of morphine
9551
Papaver somniferum
Morphine + NADP+
-
-
-
-
Naloxone + NADPH
-
9551
Papaver somniferum
?
-
-
-
-
Naltrexone + NADPH
-
9551
Papaver somniferum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
34000-35000, SDS-PAGE
Papaver somniferum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
reduction of codeinone
Papaver somniferum
46
-
oxidation of codeine
Papaver somniferum
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
29
47
50% of maximal activity at 29C and 47C, reduction of codeinone
Papaver somniferum
34
53
50% of maximal activity at 34C and 53C, oxidation of codeine
Papaver somniferum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
reduction of codeinone
Papaver somniferum
9.1
-
oxidation of codeine
Papaver somniferum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.2
7.6
50% of maximal activity at pH 6.2 and pH 7.6, reduction of codeinone
Papaver somniferum
8.2
9.8
50% of maximal activity at pH 8.2 and pH 9.8, oxidation of codeine
Papaver somniferum
Other publictions for EC 1.1.1.247
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724646
Sharafi
Metabolic engineering of morph ...
Papaver somniferum
Biotechnol. Lett.
35
445-453
2013
-
1
1
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
1
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689454
Apuya
Enhancement of alkaloid produc ...
Eschscholzia californica, Papaver somniferum
Plant Biotechnol. J.
6
160-175
2008
2
-
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689451
Larkin
Increasing morphinan alkaloid ...
Papaver somniferum
Plant Biotechnol. J.
5
26-37
2007
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670561
Samanani
The role of phloem sieve eleme ...
Papaver somniferum
Plant J.
47
547-563
2006
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670562
Ziegler
Comparative transcript and alk ...
Papaver somniferum
Plant J.
48
177-192
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660256
Alcantara
Sanguinarine biosynthesis is a ...
Papaver somniferum
Plant Physiol.
138
173-183
2005
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657166
Weid
The roles of latex and the vas ...
Papaver somniferum
Proc. Natl. Acad. Sci. USA
101
13957-13962
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
3
-
-
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-
-
-
-
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-
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3
-
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-
-
-
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-
-
-
-
656969
Bird
A tale of three cell types: al ...
Papaver somniferum
Plant Cell
15
2626-2635
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
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1
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1
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-
1
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1
-
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1
-
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-
-
-
-
-
-
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-
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-
-
-
9551
Lenz
Purification and properties of ...
Papaver somniferum
Eur. J. Biochem.
233
132-139
1995
-
-
-
-
-
-
-
15
2
-
1
2
-
2
-
-
1
-
-
5
1
-
11
1
2
2
-
-
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
15
2
-
1
2
-
-
-
1
-
5
1
-
11
1
2
2
-
-
2
2
-
-
-
-
-
-
-
-
9553
Lenz
-
Stereoselective reduction of c ...
Papaver somniferum
Tetrahedron Lett.
36
2449-2452
1995
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
2
-
-
6
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
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6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9552
Gollwitzer
-
The transformation of neopinon ...
Papaver somniferum
Tetrahedron Lett.
34
5703-5706
1993
-
-
-
-
-
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1
-
1
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2
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1
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2
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