BRENDA - Enzyme Database show
show all sequences of 1.1.1.245

Purification and characterization of a cyclohexanol dehydrogenase from Rhodococcus sp. TK6

Tae-Kang, K.; Choi, J.H.; Rhee, I.K.; J. Microbiol. Biotechnol. 12, 39-45 (2002)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Al3+
almost complete inhibition of activity at 10 mM
Rhodococcus sp.
Ca2+
inhibition of activity at 10 mM
Rhodococcus sp.
Co2+
inhibition of activity at 10 mM
Rhodococcus sp.
Cu2+
almost complete inhibition of activity at 10 mM
Rhodococcus sp.
Mn2+
93% inhibition of activity at 10 mM
Rhodococcus sp.
Zn2+
91% inhibition of activity at 10 mM
Rhodococcus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0017
-
Cyclohexanol
isoenzyme II at pH 7.6
Rhodococcus sp.
0.0028
-
trans-Cyclohexane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
0.0135
-
hexane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
0.0137
-
trans-cyclopentane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
0.0142
-
Cyclopentanol
isoenzyme II at pH 7.6
Rhodococcus sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
increase of activity at 10 mM
Rhodococcus sp.
Mg2+
increase of activity at 10 mM
Rhodococcus sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE, isoenzyme II
Rhodococcus sp.
60000
-
gel filtration, isoenzyme II
Rhodococcus sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus sp.
-
strain TK6
-
Rhodococcus sp. TK6
-
strain TK6
-
Purification (Commentary)
Commentary
Organism
9.8fold, isoenzyme II
Rhodococcus sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
127
-
purified enzyme
Rhodococcus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
butanal + NADH
-
-
-
?
1-hexanol + NAD+
relative oxidation activity at 21% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
hexanal + NADH
-
-
-
?
1-pentanol + NAD+
relative activity at 23% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
n-pentanal + NADH
-
-
-
?
1-propanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
propionaldehyde + NADH
-
-
-
?
2-butanol + NAD+
relative activity at 44% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-butanone + NADH
-
-
-
?
2-methyl-1-butanol + NAD+
relative activity at 26% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-methyl-1-butanal + NADH
-
-
-
?
2-methyl-1-propanol + NAD+
relative activity at 17% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
isobutyraldehyde + NADH
-
-
-
?
2-methyl-2-propanol + NAD+
relative activity at 16% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
2-propanol + NAD+
relative activity at 17% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
acetone + NADH
-
-
-
?
cis-cyclopentane-1,2-diol + NAD+
relative activity at 27% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis/trans mixture at 123% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
cyclohexan-1,2-diol + NAD+
relative oxidation activity of trans-isomer at 146% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
cyclohexane-1,2-dione + NADH
-
-
-
?
cyclohexane-1,3-diol + NAD+
relative oxidation activity at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,3-dione + NADH
-
-
-
?
cyclohexane-1,4-diol + NAD+
relative oxidation activity at 22% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,4-dione + NADH
-
-
-
?
cyclohexanol + NAD+
-
286383
Rhodococcus sp.
cyclohexanone + NADH
-
-
-
?
cyclohexanol + NAD+
-
286383
Rhodococcus sp. TK6
cyclohexanone + NADH
-
-
-
?
cyclopentanol + NAD+
relative oxidation activity at 77% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclopentanone + NADH
-
-
-
?
cyclopentanol + NAD+
relative oxidation activity at 77% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
cyclopentanone + NADH
-
-
-
?
ethane-1,2-diol + NAD+
relative activity at 5% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
glyoxal + NADH
-
-
-
?
ethanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
acetaldehyde + NADH
-
-
-
?
ethanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
acetaldehyde + NADH
-
-
-
?
hexane-1,2-diol + NAD+
relative activity at 84% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-oxohexanal + NADH
-
-
-
?
hexane-1,6-diol + NAD+
relative activity at 21% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
hexanedial + NADH
-
-
-
?
methanol + NAD+
relative activity at 2% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
formaldehyde + NADH + H+
-
-
-
?
methanol + NAD+
relative activity at 2% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
formaldehyde + NADH + H+
-
-
-
?
propane-1,2-diol + NAD+
relative activity at 30% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
methylglyoxal + NADH
-
-
-
?
propane-1,3-diol + NAD+
relative activity at 7% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
trans-cyclopentane-1,2-diol + NAD+
relative activity at 114% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 28000, SDS-PAGE, isoenzyme II
Rhodococcus sp.
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
labile
Rhodococcus sp.
40
-
90% activity after incubation for 20 min
Rhodococcus sp.
50
-
less than 20% activity after incubation for 20 min
Rhodococcus sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
8.5
stable
Rhodococcus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
isoenzyme II
Rhodococcus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
isoenzyme II
Rhodococcus sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Al3+
almost complete inhibition of activity at 10 mM
Rhodococcus sp.
Ca2+
inhibition of activity at 10 mM
Rhodococcus sp.
Co2+
inhibition of activity at 10 mM
Rhodococcus sp.
Cu2+
almost complete inhibition of activity at 10 mM
Rhodococcus sp.
Mn2+
93% inhibition of activity at 10 mM
Rhodococcus sp.
Zn2+
91% inhibition of activity at 10 mM
Rhodococcus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0017
-
Cyclohexanol
isoenzyme II at pH 7.6
Rhodococcus sp.
0.0028
-
trans-Cyclohexane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
0.0135
-
hexane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
0.0137
-
trans-cyclopentane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
0.0142
-
Cyclopentanol
isoenzyme II at pH 7.6
Rhodococcus sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
increase of activity at 10 mM
Rhodococcus sp.
Mg2+
increase of activity at 10 mM
Rhodococcus sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE, isoenzyme II
Rhodococcus sp.
60000
-
gel filtration, isoenzyme II
Rhodococcus sp.
Purification (Commentary) (protein specific)
Commentary
Organism
9.8fold, isoenzyme II
Rhodococcus sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
127
-
purified enzyme
Rhodococcus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
butanal + NADH
-
-
-
?
1-hexanol + NAD+
relative oxidation activity at 21% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
hexanal + NADH
-
-
-
?
1-pentanol + NAD+
relative activity at 23% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
n-pentanal + NADH
-
-
-
?
1-propanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
propionaldehyde + NADH
-
-
-
?
2-butanol + NAD+
relative activity at 44% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-butanone + NADH
-
-
-
?
2-methyl-1-butanol + NAD+
relative activity at 26% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-methyl-1-butanal + NADH
-
-
-
?
2-methyl-1-propanol + NAD+
relative activity at 17% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
isobutyraldehyde + NADH
-
-
-
?
2-methyl-2-propanol + NAD+
relative activity at 16% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
2-propanol + NAD+
relative activity at 17% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
acetone + NADH
-
-
-
?
cis-cyclopentane-1,2-diol + NAD+
relative activity at 27% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis/trans mixture at 123% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
cyclohexan-1,2-diol + NAD+
relative oxidation activity of trans-isomer at 146% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
cyclohexane-1,2-dione + NADH
-
-
-
?
cyclohexane-1,3-diol + NAD+
relative oxidation activity at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,3-dione + NADH
-
-
-
?
cyclohexane-1,4-diol + NAD+
relative oxidation activity at 22% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,4-dione + NADH
-
-
-
?
cyclohexanol + NAD+
-
286383
Rhodococcus sp.
cyclohexanone + NADH
-
-
-
?
cyclohexanol + NAD+
-
286383
Rhodococcus sp. TK6
cyclohexanone + NADH
-
-
-
?
cyclopentanol + NAD+
relative oxidation activity at 77% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclopentanone + NADH
-
-
-
?
cyclopentanol + NAD+
relative oxidation activity at 77% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
cyclopentanone + NADH
-
-
-
?
ethane-1,2-diol + NAD+
relative activity at 5% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
glyoxal + NADH
-
-
-
?
ethanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
acetaldehyde + NADH
-
-
-
?
ethanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
acetaldehyde + NADH
-
-
-
?
hexane-1,2-diol + NAD+
relative activity at 84% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-oxohexanal + NADH
-
-
-
?
hexane-1,6-diol + NAD+
relative activity at 21% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
hexanedial + NADH
-
-
-
?
methanol + NAD+
relative activity at 2% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
formaldehyde + NADH + H+
-
-
-
?
methanol + NAD+
relative activity at 2% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
formaldehyde + NADH + H+
-
-
-
?
propane-1,2-diol + NAD+
relative activity at 30% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
methylglyoxal + NADH
-
-
-
?
propane-1,3-diol + NAD+
relative activity at 7% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
trans-cyclopentane-1,2-diol + NAD+
relative activity at 114% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 28000, SDS-PAGE, isoenzyme II
Rhodococcus sp.
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
labile
Rhodococcus sp.
40
-
90% activity after incubation for 20 min
Rhodococcus sp.
50
-
less than 20% activity after incubation for 20 min
Rhodococcus sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
8.5
stable
Rhodococcus sp.
Other publictions for EC 1.1.1.245
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
667502
Giordano
Evidence for co-operativity in ...
Sulfolobus solfataricus
Biochem. J.
388
657-667
2005
-
-
-
-
1
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1
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1
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1
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667621
Secundo
Temperature-induced conformati ...
Sulfolobus solfataricus
Biochemistry
44
11040-11048
2005
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
4
1
-
1
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
4
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
286383
Tae-Kang
-
Purification and characterizat ...
Rhodococcus sp., Rhodococcus sp. TK6
J. Microbiol. Biotechnol.
12
39-45
2002
-
-
-
-
-
-
6
5
-
2
2
-
-
2
-
-
1
-
-
-
1
-
30
1
-
-
3
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
6
-
5
-
2
2
-
-
-
-
1
-
-
1
-
30
1
-
-
3
-
-
-
1
-
-
-
-
-
-
-
12556
Dangel
-
Enzyme reactions involved in a ...
Pseudomonas sp.
Arch. Microbiol.
152
273-279
1989
-
-
-
-
-
-
-
2
-
-
-
2
-
1
-
-
-
-
-
-
3
1
14
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
3
1
14
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
286378
Trower
Isolation and characterization ...
Xanthobacter sp.
Appl. Environ. Microbiol.
49
1282-1289
1985
-
-
-
-
-
-
-
4
-
-
-
1
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286381
Stirling
-
Purification and properties of ...
Nocardia sp.
Curr. Microbiol.
4
37-40
1980
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
1
-
-
-
1
-
25
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
1
-
-
1
-
25
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
286382
Anderson M.A.; Hall
-
Microbial metabolism of alicyc ...
Pseudomonas sp.
J. Gen. Microbiol.
120
89-94
1980
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
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-
-
-
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-
1
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-
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-
-
-
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-
-
-
-
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-
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-
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1
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-
1
-
-
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-
-
-
-
-
-
246371
Donoghue
The metabolism of cyclohexanol ...
Acinetobacter sp., Acinetobacter sp. NCIB
Eur. J. Biochem.
60
1-7
1975
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