BRENDA - Enzyme Database show
show all sequences of 1.1.1.245

Purification and properties of a nicotinamide adenine dinucleotide-linked cyclohexanol dehydrogenase from a Nocardia species

Stirling, L.A.; Perry, J.J.; Curr. Microbiol. 4, 37-40 (1980)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoate
100% inhibition
Nocardia sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
NAD+
-
Nocardia sp.
0.037
-
Cyclohexanol
-
Nocardia sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
145000
-
gel filtration
Nocardia sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nocardia sp.
-
-
-
Purification (Commentary)
Commentary
Organism
126fold, affinity chromatograpy
Nocardia sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
143.5
-
purified enzyme
Nocardia sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NAD+
poor substrate
286381
Nocardia sp.
butanal + NADH
-
-
-
?
1-decanol + NAD+
poor substrate
286381
Nocardia sp.
dodecanal + NADH
-
-
-
?
1-dodecanol + NAD+
poor substrate
286381
Nocardia sp.
dodecanal + NADH
-
-
-
?
1-heptanol + NAD+
poor substrate
286381
Nocardia sp.
heptanal + NADH
-
-
-
?
1-octanol + NAD+
poor substrate
286381
Nocardia sp.
octanal + NADH
-
-
-
?
2,3-butanediol + NAD+
relative activity at 4% the rate of cyclohexanol oxidation
286381
Nocardia sp.
? + NADH
-
-
-
?
2,3-dimethylcyclohexanol + NAD+
relative activity at 78% the rate of cyclohexanol oxidation
286381
Nocardia sp.
2,3-methylcyclohexanone + NADH
-
-
-
?
2,6-dimethylcyclohexanol + NAD+
relative activity at 14% the rate of cyclohexanol oxidation
286381
Nocardia sp.
2,6-methylcyclohexanone + NADH
-
-
-
?
2-methyl-1-pentanol + NAD+
poor substrate
286381
Nocardia sp.
2-methylpentanal + NADH
-
-
-
?
2-methylcyclohexanol + NAD+
relative activity at 51% the rate of cyclohexanol oxidation
286381
Nocardia sp.
2-methylcyclohexanone + NADH
-
-
-
?
2-pentanol + NAD+
relative activity at 43% the rate of cyclohexanol oxidation
286381
Nocardia sp.
2-pentanone + NADH
-
-
-
?
2-propanol + NAD+
relative activity at 19% the rate of cyclohexanol oxidation
286381
Nocardia sp.
acetone + NADH
-
-
-
?
3-cyclohexyl-1-propanol + NAD+
poor substrate
286381
Nocardia sp.
3-cyclohexyl-1-propanal + NADH
-
-
-
?
3-hexanol + NAD+
relative activity at 196% the rate of cyclohexanol oxidation
286381
Nocardia sp.
3-hexanone + NADH
-
-
-
?
3-methylcyclohexanol + NAD+
relative activity at 74% the rate of cyclohexanol oxidation
286381
Nocardia sp.
3-methylcyclohexanone + NADH
-
-
-
?
4-heptanol + NAD+
relative activity at 125% the rate of cyclohexanol oxidation
286381
Nocardia sp.
4-heptanone + NADH
-
-
-
?
4-methyl-1-pentanol + NAD+
poor substrate
286381
Nocardia sp.
4-methylpentanal + NADH
-
-
-
?
4-methylcyclohexanol + NAD+
relative activity at 66% the rate of cyclohexanol oxidation
286381
Nocardia sp.
4-methylcyclohexanone + NADH
-
-
-
?
benzyl alcohol + NAD+
poor substrate
286381
Nocardia sp.
benzaldehyde + NADH
-
-
-
?
cyclohexan-1,2-diol + NAD+
relative oxidation activity at 43% the rate of cyclohexanol oxidation
286381
Nocardia sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
cyclohexane-1,3-diol + NAD+
relative oxidation activity at 96% the rate of cyclohexanol oxidation
286381
Nocardia sp.
cyclohexane-1,3-dione + NADH
-
-
-
?
cyclohexane-1,4-diol + NAD+
relative oxidation activity at 91% the rate of cyclohexanol oxidation
286381
Nocardia sp.
cyclohexane-1,4-dione + NADH
-
-
-
?
cyclohexanol + NAD+
-
286381
Nocardia sp.
cyclohexanone + NADH
reverse reaction at pH 7
286381
Nocardia sp.
?
cyclooctanol + NAD+
relative activity at 500% the rate of cyclohexanol oxidation
286381
Nocardia sp.
cyclooctanone + NADH
-
-
-
?
cyclopentanol + NAD+
relative oxidation activity at 232% the rate of cyclohexanol oxidation
286381
Nocardia sp.
cyclopentanone + NADH
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
1 min 50% loss of activity
Nocardia sp.
55
-
1 min 85% loss of activity
Nocardia sp.
60
-
1 min 95% loss of activity
Nocardia sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.5
-
-
Nocardia sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoate
100% inhibition
Nocardia sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
NAD+
-
Nocardia sp.
0.037
-
Cyclohexanol
-
Nocardia sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
145000
-
gel filtration
Nocardia sp.
Purification (Commentary) (protein specific)
Commentary
Organism
126fold, affinity chromatograpy
Nocardia sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
143.5
-
purified enzyme
Nocardia sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NAD+
poor substrate
286381
Nocardia sp.
butanal + NADH
-
-
-
?
1-decanol + NAD+
poor substrate
286381
Nocardia sp.
dodecanal + NADH
-
-
-
?
1-dodecanol + NAD+
poor substrate
286381
Nocardia sp.
dodecanal + NADH
-
-
-
?
1-heptanol + NAD+
poor substrate
286381
Nocardia sp.
heptanal + NADH
-
-
-
?
1-octanol + NAD+
poor substrate
286381
Nocardia sp.
octanal + NADH
-
-
-
?
2,3-butanediol + NAD+
relative activity at 4% the rate of cyclohexanol oxidation
286381
Nocardia sp.
? + NADH
-
-
-
?
2,3-dimethylcyclohexanol + NAD+
relative activity at 78% the rate of cyclohexanol oxidation
286381
Nocardia sp.
2,3-methylcyclohexanone + NADH
-
-
-
?
2,6-dimethylcyclohexanol + NAD+
relative activity at 14% the rate of cyclohexanol oxidation
286381
Nocardia sp.
2,6-methylcyclohexanone + NADH
-
-
-
?
2-methyl-1-pentanol + NAD+
poor substrate
286381
Nocardia sp.
2-methylpentanal + NADH
-
-
-
?
2-methylcyclohexanol + NAD+
relative activity at 51% the rate of cyclohexanol oxidation
286381
Nocardia sp.
2-methylcyclohexanone + NADH
-
-
-
?
2-pentanol + NAD+
relative activity at 43% the rate of cyclohexanol oxidation
286381
Nocardia sp.
2-pentanone + NADH
-
-
-
?
2-propanol + NAD+
relative activity at 19% the rate of cyclohexanol oxidation
286381
Nocardia sp.
acetone + NADH
-
-
-
?
3-cyclohexyl-1-propanol + NAD+
poor substrate
286381
Nocardia sp.
3-cyclohexyl-1-propanal + NADH
-
-
-
?
3-hexanol + NAD+
relative activity at 196% the rate of cyclohexanol oxidation
286381
Nocardia sp.
3-hexanone + NADH
-
-
-
?
3-methylcyclohexanol + NAD+
relative activity at 74% the rate of cyclohexanol oxidation
286381
Nocardia sp.
3-methylcyclohexanone + NADH
-
-
-
?
4-heptanol + NAD+
relative activity at 125% the rate of cyclohexanol oxidation
286381
Nocardia sp.
4-heptanone + NADH
-
-
-
?
4-methyl-1-pentanol + NAD+
poor substrate
286381
Nocardia sp.
4-methylpentanal + NADH
-
-
-
?
4-methylcyclohexanol + NAD+
relative activity at 66% the rate of cyclohexanol oxidation
286381
Nocardia sp.
4-methylcyclohexanone + NADH
-
-
-
?
benzyl alcohol + NAD+
poor substrate
286381
Nocardia sp.
benzaldehyde + NADH
-
-
-
?
cyclohexan-1,2-diol + NAD+
relative oxidation activity at 43% the rate of cyclohexanol oxidation
286381
Nocardia sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
cyclohexane-1,3-diol + NAD+
relative oxidation activity at 96% the rate of cyclohexanol oxidation
286381
Nocardia sp.
cyclohexane-1,3-dione + NADH
-
-
-
?
cyclohexane-1,4-diol + NAD+
relative oxidation activity at 91% the rate of cyclohexanol oxidation
286381
Nocardia sp.
cyclohexane-1,4-dione + NADH
-
-
-
?
cyclohexanol + NAD+
-
286381
Nocardia sp.
cyclohexanone + NADH
reverse reaction at pH 7
286381
Nocardia sp.
?
cyclooctanol + NAD+
relative activity at 500% the rate of cyclohexanol oxidation
286381
Nocardia sp.
cyclooctanone + NADH
-
-
-
?
cyclopentanol + NAD+
relative oxidation activity at 232% the rate of cyclohexanol oxidation
286381
Nocardia sp.
cyclopentanone + NADH
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
1 min 50% loss of activity
Nocardia sp.
55
-
1 min 85% loss of activity
Nocardia sp.
60
-
1 min 95% loss of activity
Nocardia sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.5
-
-
Nocardia sp.
Other publictions for EC 1.1.1.245
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
667502
Giordano
Evidence for co-operativity in ...
Sulfolobus solfataricus
Biochem. J.
388
657-667
2005
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667621
Secundo
Temperature-induced conformati ...
Sulfolobus solfataricus
Biochemistry
44
11040-11048
2005
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1
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1
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1
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1
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4
1
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1
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286383
Tae-Kang
-
Purification and characterizat ...
Rhodococcus sp., Rhodococcus sp. TK6
J. Microbiol. Biotechnol.
12
39-45
2002
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6
5
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2
2
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2
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1
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1
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30
1
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3
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1
1
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1
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6
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5
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2
2
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1
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1
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30
1
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3
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1
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12556
Dangel
-
Enzyme reactions involved in a ...
Pseudomonas sp.
Arch. Microbiol.
152
273-279
1989
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2
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2
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1
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3
1
14
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1
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1
2
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3
1
14
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1
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1
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286378
Trower
Isolation and characterization ...
Xanthobacter sp.
Appl. Environ. Microbiol.
49
1282-1289
1985
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4
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1
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6
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1
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286381
Stirling
-
Purification and properties of ...
Nocardia sp.
Curr. Microbiol.
4
37-40
1980
-
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1
2
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1
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1
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25
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25
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3
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1
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286382
Anderson M.A.; Hall
-
Microbial metabolism of alicyc ...
Pseudomonas sp.
J. Gen. Microbiol.
120
89-94
1980
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246371
Donoghue
The metabolism of cyclohexanol ...
Acinetobacter sp., Acinetobacter sp. NCIB
Eur. J. Biochem.
60
1-7
1975
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