BRENDA - Enzyme Database show
show all sequences of 1.1.1.245

Isolation and characterization of a cyclohexane-metabolizing Xanthobacter sp.

Trower, M.K.; Buckland, R.M.; Higgins, R.; Griffin, M.; Appl. Environ. Microbiol. 49, 1282-1289 (1985)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0013
-
Cyclohexanol
pH 10.3
Xanthobacter sp.
0.41
-
Cyclohexane-1,2-diol
pH 10.3
Xanthobacter sp.
0.5
-
Cyclohexane-1,3-diol
pH 10.3
Xanthobacter sp.
0.59
-
Cyclohexane-1,4-diol
pH 10.3
Xanthobacter sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cyclohexanol + NAD+
Xanthobacter sp.
metabolism of cyclohexane
cyclohexanone + NADH
-
Xanthobacter sp.
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Xanthobacter sp.
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.87
-
-
Xanthobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cyclohexan-1,2-diol + NAD+
-
286378
Xanthobacter sp.
cyclohexane-1,2-dione + NADH
-
286378
Xanthobacter sp.
?
cyclohexane-1,3-diol + NAD+
-
286378
Xanthobacter sp.
cyclohexane-1,3-dione + NADH
-
286378
Xanthobacter sp.
?
cyclohexane-1,4-diol + NAD+
-
286378
Xanthobacter sp.
cyclohexane-1,4-dione + NADH
-
286378
Xanthobacter sp.
?
cyclohexanol + NAD+
preferred substrate
286378
Xanthobacter sp.
cyclohexanone + NADH
-
286378
Xanthobacter sp.
?
cyclohexanol + NAD+
metabolism of cyclohexane
286378
Xanthobacter sp.
cyclohexanone + NADH
-
286378
Xanthobacter sp.
r
additional information
cyclohexane-1,2-dione is not oxidized with NAD+
286378
Xanthobacter sp.
additional information
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.1
10.5
oxidation of alcohol
Xanthobacter sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Xanthobacter sp.
NADP+
weaker activity than with NAD+
Xanthobacter sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Xanthobacter sp.
NADP+
weaker activity than with NAD+
Xanthobacter sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0013
-
Cyclohexanol
pH 10.3
Xanthobacter sp.
0.41
-
Cyclohexane-1,2-diol
pH 10.3
Xanthobacter sp.
0.5
-
Cyclohexane-1,3-diol
pH 10.3
Xanthobacter sp.
0.59
-
Cyclohexane-1,4-diol
pH 10.3
Xanthobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cyclohexanol + NAD+
Xanthobacter sp.
metabolism of cyclohexane
cyclohexanone + NADH
-
Xanthobacter sp.
r
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.87
-
-
Xanthobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cyclohexan-1,2-diol + NAD+
-
286378
Xanthobacter sp.
cyclohexane-1,2-dione + NADH
-
286378
Xanthobacter sp.
?
cyclohexane-1,3-diol + NAD+
-
286378
Xanthobacter sp.
cyclohexane-1,3-dione + NADH
-
286378
Xanthobacter sp.
?
cyclohexane-1,4-diol + NAD+
-
286378
Xanthobacter sp.
cyclohexane-1,4-dione + NADH
-
286378
Xanthobacter sp.
?
cyclohexanol + NAD+
preferred substrate
286378
Xanthobacter sp.
cyclohexanone + NADH
-
286378
Xanthobacter sp.
?
cyclohexanol + NAD+
metabolism of cyclohexane
286378
Xanthobacter sp.
cyclohexanone + NADH
-
286378
Xanthobacter sp.
r
additional information
cyclohexane-1,2-dione is not oxidized with NAD+
286378
Xanthobacter sp.
additional information
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.1
10.5
oxidation of alcohol
Xanthobacter sp.
Other publictions for EC 1.1.1.245
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
667502
Giordano
Evidence for co-operativity in ...
Sulfolobus solfataricus
Biochem. J.
388
657-667
2005
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1
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1
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1
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1
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1
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1
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1
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1
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1
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667621
Secundo
Temperature-induced conformati ...
Sulfolobus solfataricus
Biochemistry
44
11040-11048
2005
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
1
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4
1
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1
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1
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1
1
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2
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1
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4
1
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1
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-
-
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-
286383
Tae-Kang
-
Purification and characterizat ...
Rhodococcus sp., Rhodococcus sp. TK6
J. Microbiol. Biotechnol.
12
39-45
2002
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-
-
-
-
-
6
5
-
2
2
-
-
2
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-
1
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1
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30
1
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3
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1
1
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1
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6
-
5
-
2
2
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1
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-
1
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30
1
-
-
3
-
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-
1
-
-
-
-
-
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-
12556
Dangel
-
Enzyme reactions involved in a ...
Pseudomonas sp.
Arch. Microbiol.
152
273-279
1989
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-
-
-
-
-
-
2
-
-
-
2
-
1
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3
1
14
-
-
-
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-
1
-
1
2
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-
-
-
2
-
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-
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-
2
-
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-
2
-
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3
1
14
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
286378
Trower
Isolation and characterization ...
Xanthobacter sp.
Appl. Environ. Microbiol.
49
1282-1289
1985
-
-
-
-
-
-
-
4
-
-
-
1
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
-
-
2
-
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-
2
-
-
-
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-
-
4
-
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-
1
-
-
-
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-
1
-
6
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
286381
Stirling
-
Purification and properties of ...
Nocardia sp.
Curr. Microbiol.
4
37-40
1980
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
1
-
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1
-
25
-
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3
-
1
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-
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1
-
2
-
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1
-
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-
-
1
-
-
1
-
25
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
286382
Anderson M.A.; Hall
-
Microbial metabolism of alicyc ...
Pseudomonas sp.
J. Gen. Microbiol.
120
89-94
1980
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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246371
Donoghue
The metabolism of cyclohexanol ...
Acinetobacter sp., Acinetobacter sp. NCIB
Eur. J. Biochem.
60
1-7
1975
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2
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6
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1
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4
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1
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1
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2
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1
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4
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