BRENDA - Enzyme Database show
show all sequences of 1.1.1.245

Enzyme reactions involved in anaerobic cyclohexanol metabolism by a denitrifying Pseudomonas species

Dangel, W.; Tschech, A.; Fuchs, G.; Arch. Microbiol. 152, 273-279 (1989)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NAD+
-
Pseudomonas sp.
0.05
-
Cyclohexanol
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cyclohexanol + NAD+
Pseudomonas sp.
inducible
cyclohexanone + NADH
-
-
?
cyclohexanol + NAD+
Pseudomonas sp.
metabolism of cyclohexane
cyclohexanone + NADH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.8
-
oxidation of cyclohexanol at pH 7.8
Pseudomonas sp.
1.9
-
reduction of cyclohexanone at pH 7.8
Pseudomonas sp.
2.5
-
pH 10
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-20C, stable for several days
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-cycloheptanediol + NAD+
relative oxidation activity at 25% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
cycloheptane-1,2-dione + NADH
-
-
-
?
1-heptanol + NAD+
relative oxidation activity at 32% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
heptanal + NADH
-
-
-
?
1-hexanol + NAD+
relative oxidation activity at 7% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
hexanal + NADH
-
-
-
?
2-cyclohexanol + NAD+
relative oxidation activity at 142% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
2-cyclohexenone + NADH
slow reduction
12556
Pseudomonas sp.
r
2-hydroxycyclohexanone + NADH
relative activity at 50% the rate of cyclohexanone reduction
12556
Pseudomonas sp.
? + NAD+
-
-
-
-
cycloheptanone + NADH
relative activity at 10% the rate of cyclohexanone reduction
12556
Pseudomonas sp.
cycloheptanol + NAD+
-
-
-
-
cyclohexan-1,2-diol + NAD+
relative oxidation activity at 15% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
cyclohexane-1,2-dione + NADH
slow reduction
12556
Pseudomonas sp.
r
cyclohexane-1,3-diol + NAD+
relative oxidation activity at 14% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
cyclohexane-1,3-dione + NADH
slow reduction
12556
Pseudomonas sp.
r
cyclohexane-1,4-diol + NAD+
relative oxidation activity at 36% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
cyclohexane-1,4-dione + NADH
relative reduction activity at 240% the rate of cyclohexanone reduction
12556
Pseudomonas sp.
r
cyclohexanol + NAD+
-
12556
Pseudomonas sp.
cyclohexanone + NADH
-
12556
Pseudomonas sp.
r
cyclohexanol + NAD+
inducible
12556
Pseudomonas sp.
cyclohexanone + NADH
-
-
-
?
cyclohexanol + NAD+
metabolism of cyclohexane
12556
Pseudomonas sp.
cyclohexanone + NADH
-
-
-
?
cyclopentanol + NAD+
-
12556
Pseudomonas sp.
cyclopentanone + NADH
relative reduction activity at 4% the rate of cyclohexanone reduction
12556
Pseudomonas sp.
r
additional information
2-hydroxycyclohexanone, 1,3,5-trihydroxycyclohexane, menthol, ethanol, 1-propanol, 1-butanol and 1-pentanol are not oxidized with NAD+
12556
Pseudomonas sp.
additional information
2-cyclopentenone, 1,3-cyclohexanedione, 1,3-cyclopentanedione and 5-oxocapronate are not reduced with NADH
12556
Pseudomonas sp.
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
10.5
-
rapid inactivation above pH 10.5
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
NADP+
less than 5% activity than with NAD+
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
NADP+
less than 5% activity than with NAD+
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NAD+
-
Pseudomonas sp.
0.05
-
Cyclohexanol
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cyclohexanol + NAD+
Pseudomonas sp.
inducible
cyclohexanone + NADH
-
-
?
cyclohexanol + NAD+
Pseudomonas sp.
metabolism of cyclohexane
cyclohexanone + NADH
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.8
-
oxidation of cyclohexanol at pH 7.8
Pseudomonas sp.
1.9
-
reduction of cyclohexanone at pH 7.8
Pseudomonas sp.
2.5
-
pH 10
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20C, stable for several days
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-cycloheptanediol + NAD+
relative oxidation activity at 25% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
cycloheptane-1,2-dione + NADH
-
-
-
?
1-heptanol + NAD+
relative oxidation activity at 32% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
heptanal + NADH
-
-
-
?
1-hexanol + NAD+
relative oxidation activity at 7% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
hexanal + NADH
-
-
-
?
2-cyclohexanol + NAD+
relative oxidation activity at 142% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
2-cyclohexenone + NADH
slow reduction
12556
Pseudomonas sp.
r
2-hydroxycyclohexanone + NADH
relative activity at 50% the rate of cyclohexanone reduction
12556
Pseudomonas sp.
? + NAD+
-
-
-
-
cycloheptanone + NADH
relative activity at 10% the rate of cyclohexanone reduction
12556
Pseudomonas sp.
cycloheptanol + NAD+
-
-
-
-
cyclohexan-1,2-diol + NAD+
relative oxidation activity at 15% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
cyclohexane-1,2-dione + NADH
slow reduction
12556
Pseudomonas sp.
r
cyclohexane-1,3-diol + NAD+
relative oxidation activity at 14% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
cyclohexane-1,3-dione + NADH
slow reduction
12556
Pseudomonas sp.
r
cyclohexane-1,4-diol + NAD+
relative oxidation activity at 36% the rate of cyclohexanol oxidation
12556
Pseudomonas sp.
cyclohexane-1,4-dione + NADH
relative reduction activity at 240% the rate of cyclohexanone reduction
12556
Pseudomonas sp.
r
cyclohexanol + NAD+
-
12556
Pseudomonas sp.
cyclohexanone + NADH
-
12556
Pseudomonas sp.
r
cyclohexanol + NAD+
inducible
12556
Pseudomonas sp.
cyclohexanone + NADH
-
-
-
?
cyclohexanol + NAD+
metabolism of cyclohexane
12556
Pseudomonas sp.
cyclohexanone + NADH
-
-
-
?
cyclopentanol + NAD+
-
12556
Pseudomonas sp.
cyclopentanone + NADH
relative reduction activity at 4% the rate of cyclohexanone reduction
12556
Pseudomonas sp.
r
additional information
2-hydroxycyclohexanone, 1,3,5-trihydroxycyclohexane, menthol, ethanol, 1-propanol, 1-butanol and 1-pentanol are not oxidized with NAD+
12556
Pseudomonas sp.
additional information
2-cyclopentenone, 1,3-cyclohexanedione, 1,3-cyclopentanedione and 5-oxocapronate are not reduced with NADH
12556
Pseudomonas sp.
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
10.5
-
rapid inactivation above pH 10.5
Pseudomonas sp.
Other publictions for EC 1.1.1.245
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
667502
Giordano
Evidence for co-operativity in ...
Sulfolobus solfataricus
Biochem. J.
388
657-667
2005
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667621
Secundo
Temperature-induced conformati ...
Sulfolobus solfataricus
Biochemistry
44
11040-11048
2005
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
4
1
-
1
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
4
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
286383
Tae-Kang
-
Purification and characterizat ...
Rhodococcus sp., Rhodococcus sp. TK6
J. Microbiol. Biotechnol.
12
39-45
2002
-
-
-
-
-
-
6
5
-
2
2
-
-
2
-
-
1
-
-
-
1
-
30
1
-
-
3
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
6
-
5
-
2
2
-
-
-
-
1
-
-
1
-
30
1
-
-
3
-
-
-
1
-
-
-
-
-
-
-
12556
Dangel
-
Enzyme reactions involved in a ...
Pseudomonas sp.
Arch. Microbiol.
152
273-279
1989
-
-
-
-
-
-
-
2
-
-
-
2
-
1
-
-
-
-
-
-
3
1
14
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
3
1
14
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
286378
Trower
Isolation and characterization ...
Xanthobacter sp.
Appl. Environ. Microbiol.
49
1282-1289
1985
-
-
-
-
-
-
-
4
-
-
-
1
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286381
Stirling
-
Purification and properties of ...
Nocardia sp.
Curr. Microbiol.
4
37-40
1980
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
1
-
-
-
1
-
25
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
1
-
-
1
-
25
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
286382
Anderson M.A.; Hall
-
Microbial metabolism of alicyc ...
Pseudomonas sp.
J. Gen. Microbiol.
120
89-94
1980
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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1
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1
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-
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-
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-
1
-
-
-
-
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-
1
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-
-
-
-
-
-
246371
Donoghue
The metabolism of cyclohexanol ...
Acinetobacter sp., Acinetobacter sp. NCIB
Eur. J. Biochem.
60
1-7
1975
-
-
-
-
-
-
-
-
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2
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6
-
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-
1
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4
-
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1
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1
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2
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1
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4
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