BRENDA - Enzyme Database show
show all sequences of 1.1.1.244

Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties

Krog, A.; Heggeset, T.M.; Mueller, J.E.; Kupper, C.E.; Schneider, O.; Vorholt, J.A.; Ellingsen, T.E.; Brautaset, T.; PLoS ONE 8, e59188 (2013)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
activator protein ACT
all three isozymes are activated by ACT, which is encoded by gene act, overview
Bacillus methanolicus
NudF protein
can be catalytically stimulated by the Bacillus subtilis NudF protein in vitro
Bacillus methanolicus
Cloned(Commentary)
Commentary
Organism
three isozymes, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain ER2566
Bacillus methanolicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
formaldehyde
pH 6.7, 45C, isozyme Mdh3
Bacillus methanolicus
1.1
-
formaldehyde
pH 6.7, 45C, isozyme Mdh1
Bacillus methanolicus
3
-
formaldehyde
pH 6.7, 45C, isozyme Mdh1
Bacillus methanolicus
4.5
-
formaldehyde
pH 6.7, 45C, isozyme Mdh2
Bacillus methanolicus
7
-
formaldehyde
pH 6.7, 45C, isozyme Mdh2
Bacillus methanolicus
7.1
-
formaldehyde
pH 6.7, 45C, isozyme Mdh3
Bacillus methanolicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + NAD+
Bacillus methanolicus
-
formaldehyde + NADH + H+
-
-
r
methanol + NAD+
Bacillus methanolicus PB1
-
formaldehyde + NADH + H+
-
-
r
methanol + NAD+
Bacillus methanolicus MGA3
-
formaldehyde + NADH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus methanolicus
-
ATCC53907, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid; NCIMB13113, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid
-
Bacillus methanolicus MGA3
-
ATCC53907, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid
-
Bacillus methanolicus PB1
-
NCIMB13113, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,3-propandiol + NAD+
very low activity
726329
Bacillus methanolicus
? + NADH + H+
-
-
-
?
1,3-propandiol + NAD+
low activity
726329
Bacillus methanolicus
? + NADH + H+
-
-
-
?
butanol + NAD+
-
726329
Bacillus methanolicus
butanal + NADH + H+
-
-
-
?
ethanol + NAD+
-
726329
Bacillus methanolicus
acetaldehyde + NADH + H+
-
-
-
r
formaldehyde + NADH + H+
Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus
methanol + NAD+
-
-
-
r
formaldehyde + NADH + H+
Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus PB1
methanol + NAD+
-
-
-
r
formaldehyde + NADH + H+
Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus MGA3
methanol + NAD+
-
-
-
r
isopropanol + NAD+
low activity
726329
Bacillus methanolicus
isopropanal + NADH + H+
-
-
-
?
methanol + NAD+
-
726329
Bacillus methanolicus
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
-
726329
Bacillus methanolicus PB1
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus PB1
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus PB1
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
-
726329
Bacillus methanolicus MGA3
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus MGA3
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus MGA3
formaldehyde + NADH + H+
-
-
-
r
additional information
all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
726329
Bacillus methanolicus
?
-
-
-
-
additional information
all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
726329
Bacillus methanolicus PB1
?
-
-
-
-
additional information
all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
726329
Bacillus methanolicus MGA3
?
-
-
-
-
propanol + NAD+
-
726329
Bacillus methanolicus
propanal + NADH + H+
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
activity optimum at about 45C for all isozymes
Bacillus methanolicus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
-
20 min, the isozymes are all stable up to; 20 min, the isozymes are stable up to, except for isozyme Mdh1, which shows about 35-40% loss of activity
Bacillus methanolicus
60
-
20 min, isozyme Mdh1 is inactivated, isozyme Mdh2 loses about 80% of activity, while isozyme Mdh3 retains over 80% of its activity; 20 min, isozyme Mdh1 loses 90% activity, isozyme Mdh2 loses 80% of activity, while isozyme Mdh3 retains 60% of its activity
Bacillus methanolicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.7
-
formaldehyde reduction assay at
Bacillus methanolicus
9.5
-
alcohol oxidation reaction assay at
Bacillus methanolicus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Bacillus methanolicus
NADH
-
Bacillus methanolicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
activator protein ACT
all three isozymes are activated by ACT, which is encoded by gene act, overview
Bacillus methanolicus
NudF protein
can be catalytically stimulated by the Bacillus subtilis NudF protein in vitro
Bacillus methanolicus
Cloned(Commentary) (protein specific)
Commentary
Organism
three isozymes, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain ER2566
Bacillus methanolicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Bacillus methanolicus
NADH
-
Bacillus methanolicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
formaldehyde
pH 6.7, 45C, isozyme Mdh3
Bacillus methanolicus
1.1
-
formaldehyde
pH 6.7, 45C, isozyme Mdh1
Bacillus methanolicus
3
-
formaldehyde
pH 6.7, 45C, isozyme Mdh1
Bacillus methanolicus
4.5
-
formaldehyde
pH 6.7, 45C, isozyme Mdh2
Bacillus methanolicus
7
-
formaldehyde
pH 6.7, 45C, isozyme Mdh2
Bacillus methanolicus
7.1
-
formaldehyde
pH 6.7, 45C, isozyme Mdh3
Bacillus methanolicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + NAD+
Bacillus methanolicus
-
formaldehyde + NADH + H+
-
-
r
methanol + NAD+
Bacillus methanolicus PB1
-
formaldehyde + NADH + H+
-
-
r
methanol + NAD+
Bacillus methanolicus MGA3
-
formaldehyde + NADH + H+
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,3-propandiol + NAD+
very low activity
726329
Bacillus methanolicus
? + NADH + H+
-
-
-
?
1,3-propandiol + NAD+
low activity
726329
Bacillus methanolicus
? + NADH + H+
-
-
-
?
butanol + NAD+
-
726329
Bacillus methanolicus
butanal + NADH + H+
-
-
-
?
ethanol + NAD+
-
726329
Bacillus methanolicus
acetaldehyde + NADH + H+
-
-
-
r
formaldehyde + NADH + H+
Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus
methanol + NAD+
-
-
-
r
formaldehyde + NADH + H+
Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus PB1
methanol + NAD+
-
-
-
r
formaldehyde + NADH + H+
Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus MGA3
methanol + NAD+
-
-
-
r
isopropanol + NAD+
low activity
726329
Bacillus methanolicus
isopropanal + NADH + H+
-
-
-
?
methanol + NAD+
-
726329
Bacillus methanolicus
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
-
726329
Bacillus methanolicus PB1
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus PB1
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus PB1
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
-
726329
Bacillus methanolicus MGA3
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus MGA3
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
726329
Bacillus methanolicus MGA3
formaldehyde + NADH + H+
-
-
-
r
additional information
all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
726329
Bacillus methanolicus
?
-
-
-
-
additional information
all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
726329
Bacillus methanolicus PB1
?
-
-
-
-
additional information
all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
726329
Bacillus methanolicus MGA3
?
-
-
-
-
propanol + NAD+
-
726329
Bacillus methanolicus
propanal + NADH + H+
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
activity optimum at about 45C for all isozymes
Bacillus methanolicus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
-
20 min, the isozymes are all stable up to; 20 min, the isozymes are stable up to, except for isozyme Mdh1, which shows about 35-40% loss of activity
Bacillus methanolicus
60
-
20 min, isozyme Mdh1 is inactivated, isozyme Mdh2 loses about 80% of activity, while isozyme Mdh3 retains over 80% of its activity; 20 min, isozyme Mdh1 loses 90% activity, isozyme Mdh2 loses 80% of activity, while isozyme Mdh3 retains 60% of its activity
Bacillus methanolicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.7
-
formaldehyde reduction assay at
Bacillus methanolicus
9.5
-
alcohol oxidation reaction assay at
Bacillus methanolicus
General Information
General Information
Commentary
Organism
evolution
strain MGA3 contains three isozymes that belong to the type III Fe-NAD+-dependent alcohol dehydrogenases, but show a distinct substrate specificity and major differences with respect to transcriptional regulation of the paralogous genes; strain PB1 contains three isozymes that belong to the type III Fe-NAD+-dependent alcohol dehydrogenases, but show a distinct substrate specificity and major differences with respect to transcriptional regulation of the paralogous genes
Bacillus methanolicus
physiological function
biological significance of Mdh for methanol oxidation during methylotrophic growth and biological role of the enzyme as part of a formaldehyde detoxification system in the methanol consuming cells
Bacillus methanolicus
General Information (protein specific)
General Information
Commentary
Organism
evolution
strain MGA3 contains three isozymes that belong to the type III Fe-NAD+-dependent alcohol dehydrogenases, but show a distinct substrate specificity and major differences with respect to transcriptional regulation of the paralogous genes; strain PB1 contains three isozymes that belong to the type III Fe-NAD+-dependent alcohol dehydrogenases, but show a distinct substrate specificity and major differences with respect to transcriptional regulation of the paralogous genes
Bacillus methanolicus
physiological function
biological significance of Mdh for methanol oxidation during methylotrophic growth and biological role of the enzyme as part of a formaldehyde detoxification system in the methanol consuming cells
Bacillus methanolicus
Other publictions for EC 1.1.1.244
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726329
Krog
Methylotrophic Bacillus methan ...
Bacillus methanolicus, Bacillus methanolicus MGA3, Bacillus methanolicus PB1
PLoS ONE
8
e59188
2013
2
-
1
-
-
-
-
6
1
-
-
3
-
10
-
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-
-
-
-
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21
-
1
-
2
-
2
-
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2
-
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2
-
1
2
-
-
-
-
-
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6
1
-
-
3
-
-
-
-
-
-
-
-
21
-
1
-
2
-
2
-
-
-
-
2
2
-
-
-
699292
Suzuki
MxaF gene, a gene encoding alp ...
Acidomonas methanolica, no activity in Acetobacter lovaniensis, no activity in Acetobacter malorum, no activity in Acetobacter malorum DSM14337T, no activity in Acetobacter pomorum, no activity in Acetobacter pomorum DSM11825T
J. Gen. Appl. Microbiol.
55
101-110
2009
-
-
-
-
-
-
-
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9
-
-
-
-
-
-
-
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1
-
-
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-
-
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-
-
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-
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-
-
-
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-
-
-
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695748
Jewell
Methylotrophy in freshwater Be ...
Beggiatoa alba
Appl. Environ. Microbiol.
74
5575-5578
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
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1
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697644
Neufeld
Marine methylotrophs revealed ...
Methylophaga spp.
Environ. Microbiol.
10
1526-1535
2008
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687193
Wang
Methylobacterium salsuginis sp ...
Methylobacterium lusitanum, Methylobacterium lusitanum MP2, Methylobacterium oryzae, Methylobacterium oryzae MP1, Methylobacterium salsuginis
Int. J. Syst. Evol. Microbiol.
57
1699-1703
2007
-
-
3
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
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-
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-
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3
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667705
Myronova
Three-dimensional structure de ...
Methylococcus capsulatus
Biochemistry
45
11905-11914
2006
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668045
Kim
-
Purification and characterizat ...
Methylomicrobium sp., Methylomicrobium sp. HG-1
Biotechnol. Bioprocess Eng.
11
134-139
2006
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
-
-
-
4
1
1
-
1
-
1
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
4
1
1
-
1
-
1
-
-
1
-
-
-
-
-
-
654389
Brantner
Intracellular localization of ...
Methylomicrobium album, Methylomicrobium album BG8
Arch. Microbiol.
178
59-64
2002
-
-
-
-
-
-
-
-
2
-
2
2
-
6
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
2
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656074
Kloosterman
Molecular, biochemical, and fu ...
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Identification of a magnesium- ...
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Environmental regulation of al ...
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