BRENDA - Enzyme Database show
show all sequences of 1.1.1.244

Identification of a magnesium-dependent NAD(P)(H)-binding domain in the nicotinoprotein methanol dehydrogenase from Bacillus methanolicus

Hektor, H.J.; Kloosterman, H.; Dijkhuizen, L.; J. Biol. Chem. 277, 46966-46973 (2002)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
strongly stimulated by the ACT protein
Bacillus methanolicus
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli DH5alpha
Bacillus methanolicus
Engineering
Amino acid exchange
Commentary
Organism
D100N
strongly reduced NAD+-binding, no activity
Bacillus methanolicus
D88N
only minor effects on activity
Bacillus methanolicus
G13A
only minor effects on activity
Bacillus methanolicus
G15A
only minor effects on activity
Bacillus methanolicus
G95A
impaired cofactor binding, low acitivity
Bacillus methanolicus
K103R
strongly reduced NAD+-binding, no activity
Bacillus methanolicus
S97G
impaired cofactor binding, much higher acitivity than the wild type enzyme, no activation with ACT protein
Bacillus methanolicus
S97T
impaired cofactor binding, much higher acitivity than the wild type enzyme, no activation with ACT protein
Bacillus methanolicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NAD+
native enzyme, pH 9.5, 50C
Bacillus methanolicus
0.04
-
NAD+
recombinant enzyme without added Mg2+, pH 9.5, 50C
Bacillus methanolicus
0.2
-
NAD+
S97T mutant enzyme without added Mg2+, pH 9.5, 50C
Bacillus methanolicus
2.5
-
NAD+
S97G mutant enzyme without added Mg2+, pH 9.5, 50C
Bacillus methanolicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
bound to enzyme, essential for binding of NAD+
Bacillus methanolicus
Zn2+
bound to enzyme
Bacillus methanolicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
10 * 43000, SDS-PAGE
Bacillus methanolicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + NAD+
Bacillus methanolicus
-
formaldehyde + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus methanolicus
-
-
-
Purification (Commentary)
Commentary
Organism
wild type and mutant enzymes
Bacillus methanolicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methanol + NAD+
-
656101
Bacillus methanolicus
formaldehyde + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
decamer
10 * 43000, SDS-PAGE
Bacillus methanolicus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
tightly but not covalentely bound to enzyme, a second NAD+ is required for the regeneration of bound NADH
Bacillus methanolicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
strongly stimulated by the ACT protein
Bacillus methanolicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli DH5alpha
Bacillus methanolicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
tightly but not covalentely bound to enzyme, a second NAD+ is required for the regeneration of bound NADH
Bacillus methanolicus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D100N
strongly reduced NAD+-binding, no activity
Bacillus methanolicus
D88N
only minor effects on activity
Bacillus methanolicus
G13A
only minor effects on activity
Bacillus methanolicus
G15A
only minor effects on activity
Bacillus methanolicus
G95A
impaired cofactor binding, low acitivity
Bacillus methanolicus
K103R
strongly reduced NAD+-binding, no activity
Bacillus methanolicus
S97G
impaired cofactor binding, much higher acitivity than the wild type enzyme, no activation with ACT protein
Bacillus methanolicus
S97T
impaired cofactor binding, much higher acitivity than the wild type enzyme, no activation with ACT protein
Bacillus methanolicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NAD+
native enzyme, pH 9.5, 50C
Bacillus methanolicus
0.04
-
NAD+
recombinant enzyme without added Mg2+, pH 9.5, 50C
Bacillus methanolicus
0.2
-
NAD+
S97T mutant enzyme without added Mg2+, pH 9.5, 50C
Bacillus methanolicus
2.5
-
NAD+
S97G mutant enzyme without added Mg2+, pH 9.5, 50C
Bacillus methanolicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
bound to enzyme, essential for binding of NAD+
Bacillus methanolicus
Zn2+
bound to enzyme
Bacillus methanolicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
10 * 43000, SDS-PAGE
Bacillus methanolicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + NAD+
Bacillus methanolicus
-
formaldehyde + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
wild type and mutant enzymes
Bacillus methanolicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methanol + NAD+
-
656101
Bacillus methanolicus
formaldehyde + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
decamer
10 * 43000, SDS-PAGE
Bacillus methanolicus
Other publictions for EC 1.1.1.244
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726329
Krog
Methylotrophic Bacillus methan ...
Bacillus methanolicus, Bacillus methanolicus MGA3, Bacillus methanolicus PB1
PLoS ONE
8
e59188
2013
2
-
1
-
-
-
-
6
1
-
-
3
-
10
-
-
-
-
-
-
-
-
21
-
1
-
2
-
2
-
-
2
-
-
-
2
-
1
2
-
-
-
-
-
-
6
1
-
-
3
-
-
-
-
-
-
-
-
21
-
1
-
2
-
2
-
-
-
-
2
2
-
-
-
699292
Suzuki
MxaF gene, a gene encoding alp ...
Acidomonas methanolica, no activity in Acetobacter lovaniensis, no activity in Acetobacter malorum, no activity in Acetobacter malorum DSM14337T, no activity in Acetobacter pomorum, no activity in Acetobacter pomorum DSM11825T
J. Gen. Appl. Microbiol.
55
101-110
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
1
-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695748
Jewell
Methylotrophy in freshwater Be ...
Beggiatoa alba
Appl. Environ. Microbiol.
74
5575-5578
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
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1
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-
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-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697644
Neufeld
Marine methylotrophs revealed ...
Methylophaga spp.
Environ. Microbiol.
10
1526-1535
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687193
Wang
Methylobacterium salsuginis sp ...
Methylobacterium lusitanum, Methylobacterium lusitanum MP2, Methylobacterium oryzae, Methylobacterium oryzae MP1, Methylobacterium salsuginis
Int. J. Syst. Evol. Microbiol.
57
1699-1703
2007
-
-
3
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667705
Myronova
Three-dimensional structure de ...
Methylococcus capsulatus
Biochemistry
45
11905-11914
2006
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668045
Kim
-
Purification and characterizat ...
Methylomicrobium sp., Methylomicrobium sp. HG-1
Biotechnol. Bioprocess Eng.
11
134-139
2006
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
-
-
-
4
1
1
-
1
-
1
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
4
1
1
-
1
-
1
-
-
1
-
-
-
-
-
-
654389
Brantner
Intracellular localization of ...
Methylomicrobium album, Methylomicrobium album BG8
Arch. Microbiol.
178
59-64
2002
-
-
-
-
-
-
-
-
2
-
2
2
-
6
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
2
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656074
Kloosterman
Molecular, biochemical, and fu ...
Bacillus methanolicus, Bacillus methanolicus C1
J. Biol. Chem.
277
34785-34792
2002
1
-
1
-
-
-
-
2
1
2
1
2
-
2
-
-
1
1
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
2
1
2
1
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656101
Hektor
Identification of a magnesium- ...
Bacillus methanolicus
J. Biol. Chem.
277
46966-46973
2002
1
-
1
-
8
-
-
4
-
2
1
1
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
8
-
-
-
-
4
-
2
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286377
Arfman
Properties of an NAD(H)-contai ...
Bacillus methanolicus
Eur. J. Biochem.
244
426-433
1997
1
-
-
-
-
-
-
-
-
-
-
-
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3
-
-
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1
-
-
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-
-
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-
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-
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1
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1
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1
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286376
Arfman
Environmental regulation of al ...
Bacillus methanolicus
Arch. Microbiol.
157
272-278
1992
1
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
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-
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1
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1
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1
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-
286374
Vonck
Electron microscopic analysis ...
Bacillus methanolicus
J. Biol. Chem.
266
3949-3954
1991
-
-
-
-
-
1
4
-
1
2
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1
-
7
-
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1
-
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-
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1
2
1
-
-
-
-
-
-
-
1
-
-
-
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-
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1
-
-
1
-
4
-
-
1
2
-
1
-
-
-
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286375
Arfman
Purification and characterizat ...
Bacillus methanolicus
J. Biol. Chem.
266
3955-3960
1991
1
-
-
-
-
-
-
1
1
1
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1
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3
-
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1
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1
2
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1
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3
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1
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1
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1
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1
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1
1
1
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1
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1
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1
2
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
286373
Arfman
Methanol dehydrogenase from th ...
Bacillus methanolicus
Methods Enzymol.
188
223-226
1990
-
-
-
-
-
1
-
-
1
-
3
1
-
2
-
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1
-
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1
1
5
1
1
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1
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1
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1
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1
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1
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3
1
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1
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1
1
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286372
Arfman
Methanol metabolism in thermot ...
Bacillus methanolicus
Arch. Microbiol.
152
280-288
1989
-
-
-
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1
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2
1
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1
2
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3
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1
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1
1
6
1
1
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2
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2
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-
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2
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1
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2
1
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1
2
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1
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1
1
6
1
1
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2
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