BRENDA - Enzyme Database show
show all sequences of 1.1.1.244

Molecular, biochemical, and functional characterization of a Nudix hydrolase protein that stimulates the activity of a nicotinoprotein alcohol dehydrogenase

Kloosterman, H.; Vrijbloed, J.W.; Dijkhuizen, L.; J. Biol. Chem. 277, 34785-34792 (2002)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
strongly stimulated by the ACT protein
Bacillus methanolicus
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli, full activity only when grown in Mg2+ containing medium
Bacillus methanolicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NAD+
native enzyme, pH 9.5, 50°C
Bacillus methanolicus
0.04
-
NAD+
recombinant enzyme, pH 9.5, 50°C
Bacillus methanolicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Bacillus methanolicus
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
bound to enzyme, essential for binding of NAD+
Bacillus methanolicus
Zn2+
bound to enzyme
Bacillus methanolicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
10 * 43000
Bacillus methanolicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + NAD+
Bacillus methanolicus
-
formaldehyde + NADH + H+
-
-
?
methanol + NAD+
Bacillus methanolicus C1
-
formaldehyde + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus methanolicus
-
strain C1
-
Bacillus methanolicus C1
-
strain C1
-
Purification (Commentary)
Commentary
Organism
native and recombinant proteins
Bacillus methanolicus
Reaction
Reaction
Commentary
Organism
methanol + NAD+ = formaldehyde + NADH + H+
ping-pong mechanism in the absence of ACT protein
Bacillus methanolicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methanol + NAD+
-
656074
Bacillus methanolicus
formaldehyde + NADH + H+
-
-
-
?
methanol + NAD+
-
656074
Bacillus methanolicus C1
formaldehyde + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
decamer
10 * 43000
Bacillus methanolicus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
tightly but not covalentely bound to enzyme
Bacillus methanolicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
strongly stimulated by the ACT protein
Bacillus methanolicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli, full activity only when grown in Mg2+ containing medium
Bacillus methanolicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
tightly but not covalentely bound to enzyme
Bacillus methanolicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NAD+
native enzyme, pH 9.5, 50°C
Bacillus methanolicus
0.04
-
NAD+
recombinant enzyme, pH 9.5, 50°C
Bacillus methanolicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Bacillus methanolicus
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
bound to enzyme, essential for binding of NAD+
Bacillus methanolicus
Zn2+
bound to enzyme
Bacillus methanolicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
10 * 43000
Bacillus methanolicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + NAD+
Bacillus methanolicus
-
formaldehyde + NADH + H+
-
-
?
methanol + NAD+
Bacillus methanolicus C1
-
formaldehyde + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native and recombinant proteins
Bacillus methanolicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methanol + NAD+
-
656074
Bacillus methanolicus
formaldehyde + NADH + H+
-
-
-
?
methanol + NAD+
-
656074
Bacillus methanolicus C1
formaldehyde + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
decamer
10 * 43000
Bacillus methanolicus
Other publictions for EC 1.1.1.244
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726329
Krog
Methylotrophic Bacillus methan ...
Bacillus methanolicus, Bacillus methanolicus MGA3, Bacillus methanolicus PB1
PLoS ONE
8
e59188
2013
2
-
1
-
-
-
-
6
1
-
-
3
-
10
-
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-
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-
-
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21
-
1
-
2
-
2
-
-
2
-
-
-
2
-
1
2
-
-
-
-
-
-
6
1
-
-
3
-
-
-
-
-
-
-
-
21
-
1
-
2
-
2
-
-
-
-
2
2
-
-
-
699292
Suzuki
MxaF gene, a gene encoding alp ...
Acidomonas methanolica, no activity in Acetobacter lovaniensis, no activity in Acetobacter malorum, no activity in Acetobacter malorum DSM14337T, no activity in Acetobacter pomorum, no activity in Acetobacter pomorum DSM11825T
J. Gen. Appl. Microbiol.
55
101-110
2009
-
-
-
-
-
-
-
-
-
-
-
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9
-
-
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695748
Jewell
Methylotrophy in freshwater Be ...
Beggiatoa alba
Appl. Environ. Microbiol.
74
5575-5578
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
3
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1
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697644
Neufeld
Marine methylotrophs revealed ...
Methylophaga spp.
Environ. Microbiol.
10
1526-1535
2008
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
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1
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-
-
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1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
687193
Wang
Methylobacterium salsuginis sp ...
Methylobacterium lusitanum, Methylobacterium lusitanum MP2, Methylobacterium oryzae, Methylobacterium oryzae MP1, Methylobacterium salsuginis
Int. J. Syst. Evol. Microbiol.
57
1699-1703
2007
-
-
3
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667705
Myronova
Three-dimensional structure de ...
Methylococcus capsulatus
Biochemistry
45
11905-11914
2006
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
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1
-
-
-
-
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1
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-
-
-
-
-
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1
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-
-
-
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1
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-
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-
-
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1
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1
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-
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1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
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-
668045
Kim
-
Purification and characterizat ...
Methylomicrobium sp., Methylomicrobium sp. HG-1
Biotechnol. Bioprocess Eng.
11
134-139
2006
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
1
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-
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4
1
1
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1
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1
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1
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1
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1
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-
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-
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2
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-
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1
-
-
-
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4
1
1
-
1
-
1
-
-
1
-
-
-
-
-
-
654389
Brantner
Intracellular localization of ...
Methylomicrobium album, Methylomicrobium album BG8
Arch. Microbiol.
178
59-64
2002
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-
-
-
-
-
-
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2
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2
2
-
6
-
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4
1
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1
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1
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2
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2
2
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4
1
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-
-
-
-
-
656074
Kloosterman
Molecular, biochemical, and fu ...
Bacillus methanolicus, Bacillus methanolicus C1
J. Biol. Chem.
277
34785-34792
2002
1
-
1
-
-
-
-
2
1
2
1
2
-
2
-
-
1
1
-
-
-
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2
1
-
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-
-
-
1
-
-
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1
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1
1
-
-
-
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-
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2
1
2
1
2
-
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1
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-
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2
1
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-
-
-
-
-
-
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-
-
-
-
-
-
656101
Hektor
Identification of a magnesium- ...
Bacillus methanolicus
J. Biol. Chem.
277
46966-46973
2002
1
-
1
-
8
-
-
4
-
2
1
1
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
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1
-
-
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1
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1
1
-
8
-
-
-
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4
-
2
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286377
Arfman
Properties of an NAD(H)-contai ...
Bacillus methanolicus
Eur. J. Biochem.
244
426-433
1997
1
-
-
-
-
-
-
-
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3
-
-
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1
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1
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1
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1
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286376
Arfman
Environmental regulation of al ...
Bacillus methanolicus
Arch. Microbiol.
157
272-278
1992
1
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-
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-
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-
-
-
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1
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1
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1
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1
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286374
Vonck
Electron microscopic analysis ...
Bacillus methanolicus
J. Biol. Chem.
266
3949-3954
1991
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1
4
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1
2
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1
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7
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1
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1
2
1
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1
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1
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1
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4
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1
2
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1
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1
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1
2
1
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286375
Arfman
Purification and characterizat ...
Bacillus methanolicus
J. Biol. Chem.
266
3955-3960
1991
1
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-
-
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-
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1
1
1
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1
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3
-
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1
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1
2
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1
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3
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1
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1
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1
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1
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1
1
1
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1
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1
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1
2
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1
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3
-
1
-
-
-
-
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-
-
-
-
286373
Arfman
Methanol dehydrogenase from th ...
Bacillus methanolicus
Methods Enzymol.
188
223-226
1990
-
-
-
-
-
1
-
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1
-
3
1
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2
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1
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1
1
5
1
1
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1
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1
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1
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1
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1
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3
1
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1
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1
1
5
1
1
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1
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286372
Arfman
Methanol metabolism in thermot ...
Bacillus methanolicus
Arch. Microbiol.
152
280-288
1989
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1
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2
1
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1
2
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3
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1
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1
1
6
1
1
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2
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2
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2
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1
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2
1
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1
2
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1
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1
1
6
1
1
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2
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