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Literature summary for 1.1.1.244 extracted from

  • Arfman, N.; Van Beeumen, J.; De Vries, G.E.; Harder, W.; Dijkhuizen, L.
    Purification and characterization of an activator protein for methanol dehydrogenase from thermotolerant Bacillus sp. (1991), J. Biol. Chem., 266, 3955-3960.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Activator protein activation in presence of Mg2+, in vitro: 3 mol soluble activator protein per mol enzyme, in vivo estimated as 1 mol per 17.5 mol enzyme, MW 50000 with two subunits of MW 27000 each, in presence of activator protein and Mg2+ enzyme possesses a high affinity active site for alcohol and NAD+, up to 40fold increase of activity, primarily of Vmax, slight decrease of Km-value for methanol, enzyme/activator-interaction is dilution-sensitive, no stimulation of formaldehyde reduction Bacillus methanolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information biphasic kinetics with all alcoholic substrates and NAD+ in the presence of activator protein Bacillus methanolicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Bacillus methanolicus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, 1-2 mol per mol subunit, atomic absorption spectroscopy, activation by activator protein depends on Mg2+, Zn2+, Mn2+, Ca2+ cannot replace Mg2+ Bacillus methanolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + NAD+ Bacillus methanolicus involved in initial methanol oxidation formaldehyde + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bacillus methanolicus
-
thermotolerant methylotrophic strain C1
-

Purification (Commentary)

Purification (Comment) Organism
purification of activator protein Bacillus methanolicus

Storage Stability

Storage Stability Organism
4-20°C, complete dissociation after 48 h Bacillus methanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + NAD+ no activity with 2-propanol, 2,3-butandiol, mannitol and glycerol Bacillus methanolicus formaldehyde + NADH + H+
-
?
methanol + NAD+ involved in initial methanol oxidation Bacillus methanolicus formaldehyde + NADH + H+
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
57 59
-
Bacillus methanolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
t1/2: above 2 min Bacillus methanolicus
60
-
t1/2: 6 min Bacillus methanolicus
70
-
t1/2: 2 min Bacillus methanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
methanol oxidation Bacillus methanolicus

Cofactor

Cofactor Comment Organism Structure
NAD+ required, specific for Bacillus methanolicus