General Stability | Organism |
---|---|
dilution inactivates methanol dehydrogenase activity, not reductase activity, sucrose, 20% w/v, stabilizes formaldehyde reductase not methanol dehydrogenase activity, metal ions, such as Fe2+ and Zn2+, do not stabilize methanol dehydrogenase activity, glycerol does not stabilize methanol dehydrogenase activity, dithiothreitol stabilizes reductase, not methanol dehydrogenase activity, storage leads to dissociation | Bacillus methanolicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | reduction of aldehyde | Bacillus methanolicus | |
EDTA | reduction of aldehyde | Bacillus methanolicus | |
iodoacetate | weak inhibition of aldehyde reduction | Bacillus methanolicus | |
N-ethylmaleimide | weak inhibition of aldehyde reduction | Bacillus methanolicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bacillus methanolicus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bacillus methanolicus | |
Zn2+ | required, 1 mol per mol subunit, atomic absorption spectroscopy | Bacillus methanolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | Bacillus methanolicus | involved in initial methanol oxidation | formaldehyde + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | - |
thermotolerant methylotrophic strain C1 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus methanolicus |
Storage Stability | Organism |
---|---|
4-20°C, enzyme dissociates upon storage | Bacillus methanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | - |
Bacillus methanolicus | formaldehyde + NADH + H+ | - |
? | |
methanol + NAD+ | involved in initial methanol oxidation | Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
decamer | decameric structure detected by electron microscopic analysis | Bacillus methanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required, specific for | Bacillus methanolicus |