BRENDA - Enzyme Database show
show all sequences of 1.1.1.244

Methanol metabolism in thermotolerant methylotrophic Bacillus strains involving a novel catabolic NAD-dependent methanol dehydrogenase as a key enzyme

Arfman, N.; Watling, E.M.; Clement, W.; Van Oosterwijk, R.J.; De Vries, G.E.; Harder, W.; Attwood, M.M.; Dijkhuizen, L.; Arch. Microbiol. 152, 280-288 (1989)

Data extracted from this reference:

General Stability
General Stability
Organism
dilution inactivates methanol dehydrogenase activity, not reductase activity, sucrose, 20% w/v, stabilizes formaldehyde reductase not methanol dehydrogenase activity, metal ions, such as Fe2+ and Zn2+, do not stabilize methanol dehydrogenase activity, glycerol does not stabilize methanol dehydrogenase activity, dithiothreitol stabilizes reductase, not methanol dehydrogenase activity, storage leads to dissociation
Bacillus methanolicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
3.8 mM and 166 mM, methanol, due to two active sites biphasic kinetics for methanol, ethanol or NAD+ in cell-free extracts, not for formaldehyde
Bacillus methanolicus
2
-
formaldehyde
-
Bacillus methanolicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Bacillus methanolicus
5737
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
10 * 43000, SDS-PAGE
Bacillus methanolicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ethanol + NAD+
Bacillus methanolicus
-
ethanal + NADH
-
-
-
methanol + NAD+
Bacillus methanolicus
involved in initial methanol oxidation
formaldehyde + NADH + H+
-
Bacillus methanolicus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus methanolicus
-
thermotolerant methylotrophic strain C1
-
Purification (Commentary)
Commentary
Organism
Q-Sepharose, Phenyl-Superose, dehydrogenase activity is almost completely lost after purification, 34% reductase activity are recovered
Bacillus methanolicus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
19.6
-
formaldehyde reductase activity
Bacillus methanolicus
Storage Stability
Storage Stability
Organism
-80 to 4C, in the presence of DTT at least 4 days stable
Bacillus methanolicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethanol + NAD+
-
286372
Bacillus methanolicus
ethanal + NADH
-
-
-
-
ethanol + NAD+
best substrate
286372
Bacillus methanolicus
ethanal + NADH
-
-
-
r
methanol + NAD+
33% of activity with ethanol
286372
Bacillus methanolicus
formaldehyde + NADH + H+
-
286372
Bacillus methanolicus
r
methanol + NAD+
involved in initial methanol oxidation
286372
Bacillus methanolicus
formaldehyde + NADH + H+
-
286372
Bacillus methanolicus
r
n-butanol + NAD+
87% of activity with ethanol
286372
Bacillus methanolicus
n-butanal + NADH
-
286372
Bacillus methanolicus
r
n-propanol + NAD+
71% of activity with ethanol
286372
Bacillus methanolicus
n-propanal + NADH
-
286372
Bacillus methanolicus
r
Subunits
Subunits
Commentary
Organism
decamer
10 * 43000, SDS-PAGE
Bacillus methanolicus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Bacillus methanolicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.7
-
formaldehyde reduction
Bacillus methanolicus
9.5
-
methanol oxidation
Bacillus methanolicus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no pyrroloquinoline quinone enzyme
Bacillus methanolicus
NAD+
required, specific for
Bacillus methanolicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no pyrroloquinoline quinone enzyme
Bacillus methanolicus
NAD+
required, specific for
Bacillus methanolicus
General Stability (protein specific)
General Stability
Organism
dilution inactivates methanol dehydrogenase activity, not reductase activity, sucrose, 20% w/v, stabilizes formaldehyde reductase not methanol dehydrogenase activity, metal ions, such as Fe2+ and Zn2+, do not stabilize methanol dehydrogenase activity, glycerol does not stabilize methanol dehydrogenase activity, dithiothreitol stabilizes reductase, not methanol dehydrogenase activity, storage leads to dissociation
Bacillus methanolicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
3.8 mM and 166 mM, methanol, due to two active sites biphasic kinetics for methanol, ethanol or NAD+ in cell-free extracts, not for formaldehyde
Bacillus methanolicus
2
-
formaldehyde
-
Bacillus methanolicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Bacillus methanolicus
5737
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
10 * 43000, SDS-PAGE
Bacillus methanolicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ethanol + NAD+
Bacillus methanolicus
-
ethanal + NADH
-
-
-
methanol + NAD+
Bacillus methanolicus
involved in initial methanol oxidation
formaldehyde + NADH + H+
-
Bacillus methanolicus
r
Purification (Commentary) (protein specific)
Commentary
Organism
Q-Sepharose, Phenyl-Superose, dehydrogenase activity is almost completely lost after purification, 34% reductase activity are recovered
Bacillus methanolicus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
19.6
-
formaldehyde reductase activity
Bacillus methanolicus
Storage Stability (protein specific)
Storage Stability
Organism
-80 to 4C, in the presence of DTT at least 4 days stable
Bacillus methanolicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethanol + NAD+
-
286372
Bacillus methanolicus
ethanal + NADH
-
-
-
-
ethanol + NAD+
best substrate
286372
Bacillus methanolicus
ethanal + NADH
-
-
-
r
methanol + NAD+
33% of activity with ethanol
286372
Bacillus methanolicus
formaldehyde + NADH + H+
-
286372
Bacillus methanolicus
r
methanol + NAD+
involved in initial methanol oxidation
286372
Bacillus methanolicus
formaldehyde + NADH + H+
-
286372
Bacillus methanolicus
r
n-butanol + NAD+
87% of activity with ethanol
286372
Bacillus methanolicus
n-butanal + NADH
-
286372
Bacillus methanolicus
r
n-propanol + NAD+
71% of activity with ethanol
286372
Bacillus methanolicus
n-propanal + NADH
-
286372
Bacillus methanolicus
r
Subunits (protein specific)
Subunits
Commentary
Organism
decamer
10 * 43000, SDS-PAGE
Bacillus methanolicus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Bacillus methanolicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.7
-
formaldehyde reduction
Bacillus methanolicus
9.5
-
methanol oxidation
Bacillus methanolicus
Other publictions for EC 1.1.1.244
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726329
Krog
Methylotrophic Bacillus methan ...
Bacillus methanolicus, Bacillus methanolicus MGA3, Bacillus methanolicus PB1
PLoS ONE
8
e59188
2013
2
-
1
-
-
-
-
6
1
-
-
3
-
10
-
-
-
-
-
-
-
-
21
-
1
-
2
-
2
-
-
2
-
-
-
2
-
1
2
-
-
-
-
-
-
6
1
-
-
3
-
-
-
-
-
-
-
-
21
-
1
-
2
-
2
-
-
-
-
2
2
-
-
-
699292
Suzuki
MxaF gene, a gene encoding alp ...
Acidomonas methanolica, no activity in Acetobacter lovaniensis, no activity in Acetobacter malorum, no activity in Acetobacter malorum DSM14337T, no activity in Acetobacter pomorum, no activity in Acetobacter pomorum DSM11825T
J. Gen. Appl. Microbiol.
55
101-110
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695748
Jewell
Methylotrophy in freshwater Be ...
Beggiatoa alba
Appl. Environ. Microbiol.
74
5575-5578
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
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1
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697644
Neufeld
Marine methylotrophs revealed ...
Methylophaga spp.
Environ. Microbiol.
10
1526-1535
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
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1
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-
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687193
Wang
Methylobacterium salsuginis sp ...
Methylobacterium lusitanum, Methylobacterium lusitanum MP2, Methylobacterium oryzae, Methylobacterium oryzae MP1, Methylobacterium salsuginis
Int. J. Syst. Evol. Microbiol.
57
1699-1703
2007
-
-
3
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
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3
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-
-
-
-
-
-
-
-
-
-
-
667705
Myronova
Three-dimensional structure de ...
Methylococcus capsulatus
Biochemistry
45
11905-11914
2006
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
1
-
-
-
-
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1
-
-
-
-
-
-
-
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1
-
-
-
-
-
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1
-
-
-
-
-
-
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1
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1
-
-
-
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668045
Kim
-
Purification and characterizat ...
Methylomicrobium sp., Methylomicrobium sp. HG-1
Biotechnol. Bioprocess Eng.
11
134-139
2006
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
-
-
-
4
1
1
-
1
-
1
-
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1
-
1
-
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-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
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1
-
-
-
-
4
1
1
-
1
-
1
-
-
1
-
-
-
-
-
-
654389
Brantner
Intracellular localization of ...
Methylomicrobium album, Methylomicrobium album BG8
Arch. Microbiol.
178
59-64
2002
-
-
-
-
-
-
-
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2
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2
2
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6
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4
1
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1
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1
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2
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2
2
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4
1
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656074
Kloosterman
Molecular, biochemical, and fu ...
Bacillus methanolicus, Bacillus methanolicus C1
J. Biol. Chem.
277
34785-34792
2002
1
-
1
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-
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2
1
2
1
2
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2
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1
1
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2
1
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-
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1
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1
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1
1
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2
1
2
1
2
-
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1
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2
1
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656101
Hektor
Identification of a magnesium- ...
Bacillus methanolicus
J. Biol. Chem.
277
46966-46973
2002
1
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1
-
8
-
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4
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2
1
1
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2
-
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1
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-
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1
1
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-
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1
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1
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1
1
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8
-
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4
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2
1
1
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1
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1
1
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286377
Arfman
Properties of an NAD(H)-contai ...
Bacillus methanolicus
Eur. J. Biochem.
244
426-433
1997
1
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-
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3
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1
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1
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286376
Arfman
Environmental regulation of al ...
Bacillus methanolicus
Arch. Microbiol.
157
272-278
1992
1
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1
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286374
Vonck
Electron microscopic analysis ...
Bacillus methanolicus
J. Biol. Chem.
266
3949-3954
1991
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1
4
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1
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7
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1
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1
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1
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1
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286375
Arfman
Purification and characterizat ...
Bacillus methanolicus
J. Biol. Chem.
266
3955-3960
1991
1
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1
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1
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3
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1
2
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1
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3
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1
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286373
Arfman
Methanol dehydrogenase from th ...
Bacillus methanolicus
Methods Enzymol.
188
223-226
1990
-
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1
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1
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3
1
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1
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1
5
1
1
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1
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1
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1
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1
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1
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1
1
5
1
1
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1
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286372
Arfman
Methanol metabolism in thermot ...
Bacillus methanolicus
Arch. Microbiol.
152
280-288
1989
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1
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2
1
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1
2
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3
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1
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1
1
6
1
1
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2
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2
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-
-
2
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1
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2
1
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1
2
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1
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1
1
6
1
1
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2
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