General Stability | Organism |
---|---|
dilution inactivates methanol dehydrogenase activity, not reductase activity, sucrose, 20% w/v, stabilizes formaldehyde reductase not methanol dehydrogenase activity, metal ions, such as Fe2+ and Zn2+, do not stabilize methanol dehydrogenase activity, glycerol does not stabilize methanol dehydrogenase activity, dithiothreitol stabilizes reductase, not methanol dehydrogenase activity, storage leads to dissociation | Bacillus methanolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 3.8 mM and 166 mM, methanol, due to two active sites biphasic kinetics for methanol, ethanol or NAD+ in cell-free extracts, not for formaldehyde | Bacillus methanolicus | |
2 | - |
formaldehyde | - |
Bacillus methanolicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bacillus methanolicus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
10 * 43000, SDS-PAGE | Bacillus methanolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + NAD+ | Bacillus methanolicus | - |
ethanal + NADH | - |
? | |
methanol + NAD+ | Bacillus methanolicus | involved in initial methanol oxidation | formaldehyde + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | - |
thermotolerant methylotrophic strain C1 | - |
Purification (Comment) | Organism |
---|---|
Q-Sepharose, Phenyl-Superose, dehydrogenase activity is almost completely lost after purification, 34% reductase activity are recovered | Bacillus methanolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.6 | - |
formaldehyde reductase activity | Bacillus methanolicus |
Storage Stability | Organism |
---|---|
-80 to 4°C, in the presence of DTT at least 4 days stable | Bacillus methanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + NAD+ | - |
Bacillus methanolicus | ethanal + NADH | - |
? | |
ethanol + NAD+ | best substrate | Bacillus methanolicus | ethanal + NADH | - |
r | |
methanol + NAD+ | 33% of activity with ethanol | Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | involved in initial methanol oxidation | Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r | |
n-butanol + NAD+ | 87% of activity with ethanol | Bacillus methanolicus | n-butanal + NADH | - |
r | |
n-propanol + NAD+ | 71% of activity with ethanol | Bacillus methanolicus | n-propanal + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 43000, SDS-PAGE | Bacillus methanolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus methanolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
formaldehyde reduction | Bacillus methanolicus |
9.5 | - |
methanol oxidation | Bacillus methanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no pyrroloquinoline quinone enzyme | Bacillus methanolicus | |
NAD+ | required, specific for | Bacillus methanolicus |