Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.241 extracted from

  • Williams, D.R.; Trudgill, P.W.; Taylor, D.G.
    Metabolism of 1,8-cineole by a Rhodococcus species: Ring cleavage reactions (1989), J. Gen. Microbiol., 135, 1957-1967.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-endo-hydroxycineole + NAD(P)+ Rhodococcus sp. involved in 1,8-cineole metabolism 6-oxocineole + NAD(P)H
-
r
6-endo-hydroxycineole + NAD(P)+ Rhodococcus sp. C1 involved in 1,8-cineole metabolism 6-oxocineole + NAD(P)H
-
r

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp. C1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.62
-
6-endo-hydroxycineole Rhodococcus sp.
1.08
-
6-oxocineole Rhodococcus sp.

Storage Stability

Storage Stability Organism
-20°C, after dialysis against 1 mM KH2PO4/Na2HPO4 buffer, pH 7.1 Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-endo-hydroxycineole + NAD(P)+ 10% of activity with NADPH over NADH in reverse reaction Rhodococcus sp. 6-oxocineole + NAD(P)H
-
r
6-endo-hydroxycineole + NAD(P)+ involved in 1,8-cineole metabolism Rhodococcus sp. 6-oxocineole + NAD(P)H
-
r
6-endo-hydroxycineole + NAD(P)+ 10% of activity with NADPH over NADH in reverse reaction Rhodococcus sp. C1 6-oxocineole + NAD(P)H
-
r
6-endo-hydroxycineole + NAD(P)+ involved in 1,8-cineole metabolism Rhodococcus sp. C1 6-oxocineole + NAD(P)H
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
NADH oxidation Rhodococcus sp.
10.5
-
NAD+ reduction Rhodococcus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ NADH in reverse reaction Rhodococcus sp.
NADP+ NADPH in reverse reaction, 10% of activity over NADH Rhodococcus sp.