BRENDA - Enzyme Database show
show all sequences of 1.1.1.241

Metabolism of 1,8-cineole by a Rhodococcus species: Ring cleavage reactions

Williams, D.R.; Trudgill, P.W.; Taylor, D.G.; J. Gen. Microbiol. 135, 1957-1967 (1989)
No PubMed abstract available

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-endo-hydroxycineole + NAD(P)+
Rhodococcus sp.
involved in 1,8-cineole metabolism
6-oxocineole + NAD(P)H
-
Rhodococcus sp.
r
6-endo-hydroxycineole + NAD(P)+
Rhodococcus sp. C1
involved in 1,8-cineole metabolism
6-oxocineole + NAD(P)H
-
Rhodococcus sp. C1
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus sp.
-
strain C1
-
Rhodococcus sp. C1
-
strain C1
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.62
-
6-endo-hydroxycineole
Rhodococcus sp.
1.08
-
6-oxocineole
Rhodococcus sp.
Storage Stability
Storage Stability
Organism
-20°C, after dialysis against 1 mM KH2PO4/Na2HPO4 buffer, pH 7.1
Rhodococcus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-endo-hydroxycineole + NAD(P)+
10% of activity with NADPH over NADH in reverse reaction
286367
Rhodococcus sp.
6-oxocineole + NAD(P)H
-
286367
Rhodococcus sp.
r
6-endo-hydroxycineole + NAD(P)+
involved in 1,8-cineole metabolism
286367
Rhodococcus sp.
6-oxocineole + NAD(P)H
-
286367
Rhodococcus sp.
r
6-endo-hydroxycineole + NAD(P)+
10% of activity with NADPH over NADH in reverse reaction
286367
Rhodococcus sp. C1
6-oxocineole + NAD(P)H
-
286367
Rhodococcus sp. C1
r
6-endo-hydroxycineole + NAD(P)+
involved in 1,8-cineole metabolism
286367
Rhodococcus sp. C1
6-oxocineole + NAD(P)H
-
286367
Rhodococcus sp. C1
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
NADH oxidation
Rhodococcus sp.
10.5
-
NAD+ reduction
Rhodococcus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
NADH in reverse reaction
Rhodococcus sp.
NADP+
NADPH in reverse reaction, 10% of activity over NADH
Rhodococcus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
NADH in reverse reaction
Rhodococcus sp.
NADP+
NADPH in reverse reaction, 10% of activity over NADH
Rhodococcus sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-endo-hydroxycineole + NAD(P)+
Rhodococcus sp.
involved in 1,8-cineole metabolism
6-oxocineole + NAD(P)H
-
Rhodococcus sp.
r
6-endo-hydroxycineole + NAD(P)+
Rhodococcus sp. C1
involved in 1,8-cineole metabolism
6-oxocineole + NAD(P)H
-
Rhodococcus sp. C1
r
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.62
-
6-endo-hydroxycineole
Rhodococcus sp.
1.08
-
6-oxocineole
Rhodococcus sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, after dialysis against 1 mM KH2PO4/Na2HPO4 buffer, pH 7.1
Rhodococcus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-endo-hydroxycineole + NAD(P)+
10% of activity with NADPH over NADH in reverse reaction
286367
Rhodococcus sp.
6-oxocineole + NAD(P)H
-
286367
Rhodococcus sp.
r
6-endo-hydroxycineole + NAD(P)+
involved in 1,8-cineole metabolism
286367
Rhodococcus sp.
6-oxocineole + NAD(P)H
-
286367
Rhodococcus sp.
r
6-endo-hydroxycineole + NAD(P)+
10% of activity with NADPH over NADH in reverse reaction
286367
Rhodococcus sp. C1
6-oxocineole + NAD(P)H
-
286367
Rhodococcus sp. C1
r
6-endo-hydroxycineole + NAD(P)+
involved in 1,8-cineole metabolism
286367
Rhodococcus sp. C1
6-oxocineole + NAD(P)H
-
286367
Rhodococcus sp. C1
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
NADH oxidation
Rhodococcus sp.
10.5
-
NAD+ reduction
Rhodococcus sp.
Other publictions for EC 1.1.1.241
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286367
Williams
-
Metabolism of 1,8-cineole by a ...
Rhodococcus sp., Rhodococcus sp. C1
J. Gen. Microbiol.
135
1957-1967
1989
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