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show all sequences of 1.1.1.240

Purification and characterization of N-acetyl-D-hexosamine dehydrogenase from Pseudomonas sp. No. 53

Horiuchi, T.; Kurokawa, T.; Agric. Biol. Chem. 53, 1919-1925 (1989)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
stabilized against heating by 3 mM NAD+
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
NaN3
weak
Pseudomonas sp.
SDS
-
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
NAD+
with N-acetyl-D-glucosamine as substrate
Pseudomonas sp.
0.8
-
N-acetyl-D-galactosamine
-
Pseudomonas sp.
5.3
-
N-acetyl-D-glucosamine
-
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Hg2+
inhibitory
Pseudomonas sp.
additional information
not effected by Ca2+, Mg2+, Cd2+, Ni2+, Zn2+, Cu2+, Mn2+, Co2+
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
SDS-PAGE
Pseudomonas sp.
124000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-acetyl-D-glucosamine + NAD+
Pseudomonas sp.
-
N-acetyl-D-glucosaminate + NADH
-
-
?
N-acetyl-D-glucosamine + NAD+
Pseudomonas sp. No. 53
-
N-acetyl-D-glucosaminate + NADH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
No. 53
-
Pseudomonas sp. No. 53
-
No. 53
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.265
-
crude extract
Pseudomonas sp.
239
-
purified enzyme
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-20°C, several months, without much loss of activity
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-D-galactosamine + NAD+
-
286366
Pseudomonas sp.
N-acetyl-D-galactoseaminate + NADH
-
-
-
?
N-acetyl-D-galactosamine + NAD+
-
286366
Pseudomonas sp. No. 53
N-acetyl-D-galactoseaminate + NADH
-
-
-
?
N-acetyl-D-glucosamine + NAD+
-
286366
Pseudomonas sp.
N-acetyl-D-glucosaminate + NADH
-
-
-
?
N-acetyl-D-glucosamine + NAD+
-
286366
Pseudomonas sp. No. 53
N-acetyl-D-glucosaminate + NADH
-
-
-
?
N-acetyl-D-mannosamine + NAD+ + H2O
very poor substrate
286366
Pseudomonas sp.
N-acetyl-D-mannosaminate + NADH + H+
-
-
-
?
N-acetyl-D-mannosamine + NAD+ + H2O
very poor substrate
286366
Pseudomonas sp. No. 53
N-acetyl-D-mannosaminate + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 30000, SDS-PAGE, gel filtration
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min, unstable below pH 5 and above pH 12
Pseudomonas sp.
55
-
10 min, stable
Pseudomonas sp.
65
-
10 min, 50% loss of activity
Pseudomonas sp.
70
-
10 min, complete loss of activity
Pseudomonas sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
655
-
N-acetyl-D-glucosamine
-
Pseudomonas sp.
932
-
N-acetyl-D-galactosamine
-
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8
10.5
most stable
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
strictly specific for NAD+ as hydrogen acceptor
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
strictly specific for NAD+ as hydrogen acceptor
Pseudomonas sp.
General Stability (protein specific)
General Stability
Organism
stabilized against heating by 3 mM NAD+
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NaN3
weak
Pseudomonas sp.
SDS
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
NAD+
with N-acetyl-D-glucosamine as substrate
Pseudomonas sp.
0.8
-
N-acetyl-D-galactosamine
-
Pseudomonas sp.
5.3
-
N-acetyl-D-glucosamine
-
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Hg2+
inhibitory
Pseudomonas sp.
additional information
not effected by Ca2+, Mg2+, Cd2+, Ni2+, Zn2+, Cu2+, Mn2+, Co2+
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
SDS-PAGE
Pseudomonas sp.
124000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-acetyl-D-glucosamine + NAD+
Pseudomonas sp.
-
N-acetyl-D-glucosaminate + NADH
-
-
?
N-acetyl-D-glucosamine + NAD+
Pseudomonas sp. No. 53
-
N-acetyl-D-glucosaminate + NADH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.265
-
crude extract
Pseudomonas sp.
239
-
purified enzyme
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, several months, without much loss of activity
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-D-galactosamine + NAD+
-
286366
Pseudomonas sp.
N-acetyl-D-galactoseaminate + NADH
-
-
-
?
N-acetyl-D-galactosamine + NAD+
-
286366
Pseudomonas sp. No. 53
N-acetyl-D-galactoseaminate + NADH
-
-
-
?
N-acetyl-D-glucosamine + NAD+
-
286366
Pseudomonas sp.
N-acetyl-D-glucosaminate + NADH
-
-
-
?
N-acetyl-D-glucosamine + NAD+
-
286366
Pseudomonas sp. No. 53
N-acetyl-D-glucosaminate + NADH
-
-
-
?
N-acetyl-D-mannosamine + NAD+ + H2O
very poor substrate
286366
Pseudomonas sp.
N-acetyl-D-mannosaminate + NADH + H+
-
-
-
?
N-acetyl-D-mannosamine + NAD+ + H2O
very poor substrate
286366
Pseudomonas sp. No. 53
N-acetyl-D-mannosaminate + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 30000, SDS-PAGE, gel filtration
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min, unstable below pH 5 and above pH 12
Pseudomonas sp.
55
-
10 min, stable
Pseudomonas sp.
65
-
10 min, 50% loss of activity
Pseudomonas sp.
70
-
10 min, complete loss of activity
Pseudomonas sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
655
-
N-acetyl-D-glucosamine
-
Pseudomonas sp.
932
-
N-acetyl-D-galactosamine
-
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8
10.5
most stable
Pseudomonas sp.
Other publictions for EC 1.1.1.240
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286366
Horiuchi
-
Purification and characterizat ...
Pseudomonas sp., Pseudomonas sp. No. 53
Agric. Biol. Chem.
53
1919-1925
1989
-
-
-
-
-
1
2
3
-
2
2
2
-
2
-
-
1
-
-
-
2
1
6
1
-
-
4
2
1
-
1
1
-
-
-
-
-
-
1
-
-
1
-
2
-
3
-
2
2
2
-
-
-
1
-
-
2
1
6
1
-
-
4
2
1
-
1
-
-
-
-
-
-
-