Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.24 extracted from

  • Höppner, A.; Schomburg, D.; Niefind, K.
    Enzyme-substrate complexes of the quinate/shikimate dehydrogenase from Corynebacterium glutamicum enable new insights in substrate and cofactor binding, specificity, and discrimination (2013), Biol. Chem., 394, 1505-1516.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Corynebacterium glutamicum

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion sitting-drop method at 20°C. Atomic resolution crystal structures of the enzyme in different functional states: with bound NAD+ (binary complex) and as ternary complexes with NADH plus either shikimate or quinate Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
NAD+ 30°C, pH 7.5 Corynebacterium glutamicum
0.28
-
NAD+ 30°C, pH 9.0-9.5, cosubstrate: quinate Corynebacterium glutamicum
0.46
-
NAD+ 30°C, pH 10.0-10.5, cosubstrate: shikimate Corynebacterium glutamicum
0.87
-
NAD+ 30°C, pH 7.5 Corynebacterium glutamicum
1.6
-
L-quinate 30°C, pH 7.5 Corynebacterium glutamicum
2.4
-
L-quinate 30°C, pH 9.0-9.5 Corynebacterium glutamicum
10.2
-
shikimate 30°C, pH 7.5 Corynebacterium glutamicum
53.9
-
shikimate 30°C, pH 10.0-10.5 Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-quinate + NAD+ Corynebacterium glutamicum the enzyme is involved in the catabolic quinate metabolism required for the degradation of lignin 3-dehydroquinate + NADH + H+
-
r
L-quinate + NAD+ Corynebacterium glutamicum ATCC 13032 the enzyme is involved in the catabolic quinate metabolism required for the degradation of lignin 3-dehydroquinate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum Q9X5C9
-
-
Corynebacterium glutamicum ATCC 13032 Q9X5C9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-quinate + NAD+ the enzyme is involved in the catabolic quinate metabolism required for the degradation of lignin Corynebacterium glutamicum 3-dehydroquinate + NADH + H+
-
r
L-quinate + NAD+ the enzyme also shows activity with shikimate. Clear substrate preference of the enzyme for quinate compared with shikimate both at the pH optimum and in a physiological pH range. The enzyme is strictly NAD(H) dependent Corynebacterium glutamicum 3-dehydroquinate + NADH + H+
-
r
L-quinate + NAD+ the enzyme is involved in the catabolic quinate metabolism required for the degradation of lignin Corynebacterium glutamicum ATCC 13032 3-dehydroquinate + NADH + H+
-
r
L-quinate + NAD+ the enzyme also shows activity with shikimate. Clear substrate preference of the enzyme for quinate compared with shikimate both at the pH optimum and in a physiological pH range. The enzyme is strictly NAD(H) dependent Corynebacterium glutamicum ATCC 13032 3-dehydroquinate + NADH + H+
-
r
shikimate + NAD+ the enzyme also shows high activity with quinate. Clear substrate preference of the enzyme for quinate compared with shikimate both at the pH optimum and in a physiological pH range. The enzyme is strictly NAD(H) dependent Corynebacterium glutamicum 3-dehydroshikimate + NADH + H+
-
r
shikimate + NAD+ the enzyme also shows high activity with quinate. Clear substrate preference of the enzyme for quinate compared with shikimate both at the pH optimum and in a physiological pH range. The enzyme is strictly NAD(H) dependent Corynebacterium glutamicum ATCC 13032 3-dehydroshikimate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
cgl0424
-
Corynebacterium glutamicum
CglQSDH
-
Corynebacterium glutamicum
quinate/shikimate dehydrogenase the enzyme also shows activity with shikimate Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30.1
-
shikimate 30°C, pH 7.5 Corynebacterium glutamicum
43.7
-
NAD+ 30°C, pH 7.5 Corynebacterium glutamicum
50.8
-
L-quinate 30°C, pH 7.5 Corynebacterium glutamicum
61.1
-
NAD+ 30°C, pH 7.5 Corynebacterium glutamicum
104.9
-
L-quinate 30°C, pH 9.0-9.5 Corynebacterium glutamicum
205.8
-
NAD+ 30°C, pH 10.0-10.5, cosubstrate: shikimate Corynebacterium glutamicum
214.1
-
shikimate 30°C, pH 10.0-10.5 Corynebacterium glutamicum
223.1
-
NAD+ 30°C, pH 9.0-9.5, cosubstrate: quinate Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5 substrate: L-quinate Corynebacterium glutamicum
10 10.5 substrate: shikimate Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NAD+ strictly dependent on Corynebacterium glutamicum
NADH strictly dependent on Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1
-
shikimate 30°C, pH 7.5 Corynebacterium glutamicum
4.2
-
shikimate 30°C, pH 10.0-10.5 Corynebacterium glutamicum
33.3
-
L-quinate 30°C, pH 7.5 Corynebacterium glutamicum
44.05
-
L-quinate 30°C, pH 9.0-9.5 Corynebacterium glutamicum
71.6
-
NAD+ 30°C, pH 7.5 Corynebacterium glutamicum
329.1
-
NAD+ 30°C, pH 7.5 Corynebacterium glutamicum
464.7
-
NAD+ 30°C, pH 10.0-10.5, cosubstrate: shikimate Corynebacterium glutamicum
826.6
-
NAD+ 30°C, pH 9.0-9.5, cosubstrate: quinate Corynebacterium glutamicum