Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.239 extracted from

  • Carre, J.L.; Quemener, E.; Amet, Y.; Simon, B.; Berthou, F.; Mangin, P.; Floch, H.H.; Abalain, J.H.
    Characterization and solubization of testosterone 17beta-hydroxysteroid dehydrogenase in human hyperplastic prostate (1993), J. Steroid Biochem. Mol. Biol., 46, 265-267.
    View publication on PubMed
Search for more literature for 1.1.1.239

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 testosterone solubilized enzyme Homo sapiens
0.1
-
 NAD+ solubilized enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
testosterone + NAD+ Homo sapiens
-
 androst-4-ene-3,17-dione + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
prostate gland
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl, pH 9.0, 20% glycerol, 100 mM KCl, 5 mg/ml N-octyl glucoside, 24 h, 20% loss of activity, -20°C, 1 week, no loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
testosterone + NAD+
-
 Homo sapiens androst-4-ene-3,17-dione + NADH + H+
-
 ?
testosterone + NADP+ NADP is 3fold less efficient as cofactor Homo sapiens androst-4-ene-3,17-dione + NADPH + H+
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens
NADP+ NADP+ is 3fold less efficient as cofactor Homo sapiens