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show all sequences of 1.1.1.238

Characterization of NADP-dependent 12beta-hydroxysteroid dehydrogenase from Clostridium paraputrificum

Edenharder, R.; Pfutzner, A.; Biochim. Biophys. Acta 962, 362-370 (1988)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
potential application in quantification of 12beta-hydroxyl groups in di- and trisubstituted bile acids
Clostridium paraputrificum
General Stability
General Stability
Organism
most stable in presence of reducing agents e.g. dithiothreitol
Clostridium paraputrificum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0837
-
7,12-Dioxo-5beta-cholanoate
-
Clostridium paraputrificum
0.0854
-
NADPH
-
Clostridium paraputrificum
0.0895
-
3,12-Dioxo-5beta-cholanoate
-
Clostridium paraputrificum
0.138
-
7alpha-Hydroxy-3,12-dioxo-5beta-cholanoate
-
Clostridium paraputrificum
0.141
-
3alpha-Hydroxy-12-oxo-5beta-cholanoate
-
Clostridium paraputrificum
0.142
-
3,7,12-trioxotaureate
-
Clostridium paraputrificum
0.144
-
3alpha-Hydroxy-7,12-dioxo-5beta-cholanoate
-
Clostridium paraputrificum
0.171
-
3,7,12-Trioxo-5beta-cholanoate
-
Clostridium paraputrificum
0.274
-
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate
-
Clostridium paraputrificum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Clostridium paraputrificum
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
126000
-
gel filtration
Clostridium paraputrificum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium paraputrificum
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2
4.4
depending on substrate
Clostridium paraputrificum
2.2
6.4
depending on inducer
Clostridium paraputrificum
Storage Stability
Storage Stability
Organism
-20C, lyophilized powder, at least 4 weeks
Clostridium paraputrificum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,12-dioxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
12beta-hydroxy-3-oxo-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
3,7,12-trioxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
12beta-hydroxy-3,7-dioxo-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
3,7,12-trioxotaureate + NADPH
-
286353
Clostridium paraputrificum
12beta-hydroxy-3,7-dioxotaureate + NADP+
-
286353
Clostridium paraputrificum
?
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
3alpha,7alpha,12beta-trihydroxy-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
3alpha-hydroxy-12-oxo-5beta-cholanoate + NADPH
reverse reaction 10% the rate of forward reaction
286353
Clostridium paraputrificum
3alpha,12beta-dihydroxy-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
r
3alpha-hydroxy-7,12-dioxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
3alpha,12beta-dihydroxy-7-oxo-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
7,12-dioxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
12beta-hydroxy-7-oxo-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
7alpha-hydroxy-3,12-dioxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
7alpha,12beta-dihydroxy-3-oxo-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
at least 6 h
Clostridium paraputrificum
37
-
45 min, complete inactivation
Clostridium paraputrificum
50
-
10 min, 95% inactivation, 15 min complete inactivation
Clostridium paraputrificum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
oxidation of 12beta-hydroxy bile acids
Clostridium paraputrificum
10
-
reduction of 12-oxo bile acids
Clostridium paraputrificum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
11
about half maximum activity
Clostridium paraputrificum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8.5
-
most stable
Clostridium paraputrificum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Clostridium paraputrificum
NADPH
specific for, no activity with NADH
Clostridium paraputrificum
Application (protein specific)
Application
Commentary
Organism
analysis
potential application in quantification of 12beta-hydroxyl groups in di- and trisubstituted bile acids
Clostridium paraputrificum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Clostridium paraputrificum
NADPH
specific for, no activity with NADH
Clostridium paraputrificum
General Stability (protein specific)
General Stability
Organism
most stable in presence of reducing agents e.g. dithiothreitol
Clostridium paraputrificum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0837
-
7,12-Dioxo-5beta-cholanoate
-
Clostridium paraputrificum
0.0854
-
NADPH
-
Clostridium paraputrificum
0.0895
-
3,12-Dioxo-5beta-cholanoate
-
Clostridium paraputrificum
0.138
-
7alpha-Hydroxy-3,12-dioxo-5beta-cholanoate
-
Clostridium paraputrificum
0.141
-
3alpha-Hydroxy-12-oxo-5beta-cholanoate
-
Clostridium paraputrificum
0.142
-
3,7,12-trioxotaureate
-
Clostridium paraputrificum
0.144
-
3alpha-Hydroxy-7,12-dioxo-5beta-cholanoate
-
Clostridium paraputrificum
0.171
-
3,7,12-Trioxo-5beta-cholanoate
-
Clostridium paraputrificum
0.274
-
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate
-
Clostridium paraputrificum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Clostridium paraputrificum
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
126000
-
gel filtration
Clostridium paraputrificum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2
4.4
depending on substrate
Clostridium paraputrificum
2.2
6.4
depending on inducer
Clostridium paraputrificum
Storage Stability (protein specific)
Storage Stability
Organism
-20C, lyophilized powder, at least 4 weeks
Clostridium paraputrificum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,12-dioxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
12beta-hydroxy-3-oxo-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
3,7,12-trioxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
12beta-hydroxy-3,7-dioxo-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
3,7,12-trioxotaureate + NADPH
-
286353
Clostridium paraputrificum
12beta-hydroxy-3,7-dioxotaureate + NADP+
-
286353
Clostridium paraputrificum
?
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
3alpha,7alpha,12beta-trihydroxy-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
3alpha-hydroxy-12-oxo-5beta-cholanoate + NADPH
reverse reaction 10% the rate of forward reaction
286353
Clostridium paraputrificum
3alpha,12beta-dihydroxy-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
r
3alpha-hydroxy-7,12-dioxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
3alpha,12beta-dihydroxy-7-oxo-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
7,12-dioxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
12beta-hydroxy-7-oxo-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
7alpha-hydroxy-3,12-dioxo-5beta-cholanoate + NADPH
-
286353
Clostridium paraputrificum
7alpha,12beta-dihydroxy-3-oxo-5beta-cholanoate + NADP+
-
286353
Clostridium paraputrificum
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
at least 6 h
Clostridium paraputrificum
37
-
45 min, complete inactivation
Clostridium paraputrificum
50
-
10 min, 95% inactivation, 15 min complete inactivation
Clostridium paraputrificum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
oxidation of 12beta-hydroxy bile acids
Clostridium paraputrificum
10
-
reduction of 12-oxo bile acids
Clostridium paraputrificum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
11
about half maximum activity
Clostridium paraputrificum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8.5
-
most stable
Clostridium paraputrificum
Other publictions for EC 1.1.1.238
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286353
Edenharder
Characterization of NADP-depen ...
Clostridium paraputrificum
Biochim. Biophys. Acta
962
362-370
1988
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1
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1
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9
1
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1
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1
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2
1
8
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3
-
2
1
1
2
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1
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2
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1
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9
1
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1
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2
1
8
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3
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2
1
1
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