BRENDA - Enzyme Database show
show all sequences of 1.1.1.237

Novel fungal phenylpyruvate reductase belongs to D-isomer-specific 2-hydroxyacid dehydrogenase family

Fujii, T.; Shimizu, M.; Doi, Y.; Fujita, T.; Ito, T.; Miura, D.; Wariishi, H.; Takaya, N.; Biochim. Biophys. Acta 1814, 1669-1676 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Wickerhamia fluorescens
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
1 mM, less than 10% residual activity
Wickerhamia fluorescens
Fe2+
1 mM, less than 10% residual activity
Wickerhamia fluorescens
Hg2+
1 mM, less than 10% residual activity
Wickerhamia fluorescens
WO42-
1 mM, less than 10% residual activity
Wickerhamia fluorescens
Zn2+
1 mM, less than 10% residual activity
Wickerhamia fluorescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
pH 6.5, 25C
Wickerhamia fluorescens
0.1
-
NADH
pH 6.5, 25C
Wickerhamia fluorescens
0.4
-
phenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
0.64
-
hydroxyphenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
3.5
-
Hydroxypyruvate
pH 6.5, 25C
Wickerhamia fluorescens
18.9
-
glyoxylate
pH 6.5, 25C
Wickerhamia fluorescens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE, 2 * 40300, calculated
Wickerhamia fluorescens
40300
-
2 * 40000, SDS-PAGE, 2 * 40300, calculated
Wickerhamia fluorescens
75000
-
gel filtration
Wickerhamia fluorescens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Wickerhamia fluorescens
F1T2J9
-
-
Wickerhamia fluorescens TK1
F1T2J9
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
727
-
pH 6.5, 25C
Wickerhamia fluorescens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxyphenylpyruvate + NADPH + H+
-
724428
Wickerhamia fluorescens
D-(4-hydroxyphenyl)lactate + NADP+
-
-
-
?
4-hydroxyphenylpyruvate + NADPH + H+
-
724428
Wickerhamia fluorescens TK1
D-(4-hydroxyphenyl)lactate + NADP+
-
-
-
?
glyoxylate + NADPH + H+
-
724428
Wickerhamia fluorescens
glycolate + NADP+
-
-
-
?
glyoxylate + NADPH + H+
-
724428
Wickerhamia fluorescens TK1
glycolate + NADP+
-
-
-
?
hydroxypyruvate + NADH + H+
-
724428
Wickerhamia fluorescens
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
-
724428
Wickerhamia fluorescens TK1
D-glycerate + NAD+
-
-
-
?
additional information
no substrates: pyruvate, oxaloacetate or benzoylformate
724428
Wickerhamia fluorescens
?
-
-
-
-
additional information
no substrates: pyruvate, oxaloacetate or benzoylformate
724428
Wickerhamia fluorescens TK1
?
-
-
-
-
phenylpyruvate + NADPH + H+
more than 99.9% D-isomer, L-isomer below limits of detection
724428
Wickerhamia fluorescens
D-phenyllactate + NADP+
-
-
-
?
phenylpyruvate + NADPH + H+
more than 99.9% D-isomer, L-isomer below limits of detection
724428
Wickerhamia fluorescens TK1
D-phenyllactate + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE, 2 * 40300, calculated
Wickerhamia fluorescens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9.1
-
Hydroxypyruvate
pH 6.5, 25C
Wickerhamia fluorescens
19
-
glyoxylate
pH 6.5, 25C
Wickerhamia fluorescens
31
-
NADH
pH 6.5, 25C
Wickerhamia fluorescens
73
-
hydroxyphenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
121
-
NADPH
pH 6.5, 25C
Wickerhamia fluorescens
150
-
phenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Wickerhamia fluorescens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Wickerhamia fluorescens
NADH
NADPH is preferred over NADH
Wickerhamia fluorescens
NADP+
-
Wickerhamia fluorescens
NADPH
preferred over NADH
Wickerhamia fluorescens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Wickerhamia fluorescens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Wickerhamia fluorescens
NADH
NADPH is preferred over NADH
Wickerhamia fluorescens
NADP+
-
Wickerhamia fluorescens
NADPH
preferred over NADH
Wickerhamia fluorescens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
1 mM, less than 10% residual activity
Wickerhamia fluorescens
Fe2+
1 mM, less than 10% residual activity
Wickerhamia fluorescens
Hg2+
1 mM, less than 10% residual activity
Wickerhamia fluorescens
WO42-
1 mM, less than 10% residual activity
Wickerhamia fluorescens
Zn2+
1 mM, less than 10% residual activity
Wickerhamia fluorescens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
pH 6.5, 25C
Wickerhamia fluorescens
0.1
-
NADH
pH 6.5, 25C
Wickerhamia fluorescens
0.4
-
phenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
0.64
-
hydroxyphenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
3.5
-
Hydroxypyruvate
pH 6.5, 25C
Wickerhamia fluorescens
18.9
-
glyoxylate
pH 6.5, 25C
Wickerhamia fluorescens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE, 2 * 40300, calculated
Wickerhamia fluorescens
40300
-
2 * 40000, SDS-PAGE, 2 * 40300, calculated
Wickerhamia fluorescens
75000
-
gel filtration
Wickerhamia fluorescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
727
-
pH 6.5, 25C
Wickerhamia fluorescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxyphenylpyruvate + NADPH + H+
-
724428
Wickerhamia fluorescens
D-(4-hydroxyphenyl)lactate + NADP+
-
-
-
?
4-hydroxyphenylpyruvate + NADPH + H+
-
724428
Wickerhamia fluorescens TK1
D-(4-hydroxyphenyl)lactate + NADP+
-
-
-
?
glyoxylate + NADPH + H+
-
724428
Wickerhamia fluorescens
glycolate + NADP+
-
-
-
?
glyoxylate + NADPH + H+
-
724428
Wickerhamia fluorescens TK1
glycolate + NADP+
-
-
-
?
hydroxypyruvate + NADH + H+
-
724428
Wickerhamia fluorescens
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
-
724428
Wickerhamia fluorescens TK1
D-glycerate + NAD+
-
-
-
?
additional information
no substrates: pyruvate, oxaloacetate or benzoylformate
724428
Wickerhamia fluorescens
?
-
-
-
-
additional information
no substrates: pyruvate, oxaloacetate or benzoylformate
724428
Wickerhamia fluorescens TK1
?
-
-
-
-
phenylpyruvate + NADPH + H+
more than 99.9% D-isomer, L-isomer below limits of detection
724428
Wickerhamia fluorescens
D-phenyllactate + NADP+
-
-
-
?
phenylpyruvate + NADPH + H+
more than 99.9% D-isomer, L-isomer below limits of detection
724428
Wickerhamia fluorescens TK1
D-phenyllactate + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE, 2 * 40300, calculated
Wickerhamia fluorescens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9.1
-
Hydroxypyruvate
pH 6.5, 25C
Wickerhamia fluorescens
19
-
glyoxylate
pH 6.5, 25C
Wickerhamia fluorescens
31
-
NADH
pH 6.5, 25C
Wickerhamia fluorescens
73
-
hydroxyphenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
121
-
NADPH
pH 6.5, 25C
Wickerhamia fluorescens
150
-
phenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Wickerhamia fluorescens
Expression
Organism
Commentary
Expression
Wickerhamia fluorescens
presence of phenylalanine results in up to 40fold increase in transcripts
up
General Information
General Information
Commentary
Organism
physiological function
Wickerhamia fluorescens efficiently converts phenylalanine and phenylpyruvate to D-phenyllactate. These compounds up-regulate the transcription of enzyme gene pprA
Wickerhamia fluorescens
General Information (protein specific)
General Information
Commentary
Organism
physiological function
Wickerhamia fluorescens efficiently converts phenylalanine and phenylpyruvate to D-phenyllactate. These compounds up-regulate the transcription of enzyme gene pprA
Wickerhamia fluorescens
Expression (protein specific)
Organism
Commentary
Expression
Wickerhamia fluorescens
presence of phenylalanine results in up to 40fold increase in transcripts
up
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1
-
glyoxylate
pH 6.5, 25C
Wickerhamia fluorescens
2.6
-
Hydroxypyruvate
pH 6.5, 25C
Wickerhamia fluorescens
110
-
hydroxyphenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
310
-
NADH
pH 6.5, 25C
Wickerhamia fluorescens
380
-
phenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
12000
-
NADPH
pH 6.5, 25C
Wickerhamia fluorescens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1
-
glyoxylate
pH 6.5, 25C
Wickerhamia fluorescens
2.6
-
Hydroxypyruvate
pH 6.5, 25C
Wickerhamia fluorescens
110
-
hydroxyphenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
310
-
NADH
pH 6.5, 25C
Wickerhamia fluorescens
380
-
phenylpyruvate
pH 6.5, 25C
Wickerhamia fluorescens
12000
-
NADPH
pH 6.5, 25C
Wickerhamia fluorescens
Other publictions for EC 1.1.1.237
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724428
Fujii
Novel fungal phenylpyruvate re ...
Wickerhamia fluorescens, Wickerhamia fluorescens TK1
Biochim. Biophys. Acta
1814
1669-1676
2011
-
-
1
-
-
-
5
6
-
-
3
-
-
2
-
-
-
-
-
-
1
-
10
1
-
-
-
6
1
-
-
4
-
-
-
-
-
1
4
-
-
-
-
5
-
6
-
-
3
-
-
-
-
-
-
-
1
-
10
1
-
-
-
6
1
-
-
-
1
1
1
1
6
6
723825
Janiak
Structure and substrate dockin ...
Solenostemon scutellarioides
Acta Crystallogr. Sect. D
66
593-603
2010
-
-
1
1
-
-
-
-
-
-
1
1
-
3
-
-
1
-
-
1
-
-
5
2
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
1
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
5
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
700368
Qian
Influence of growth regulators ...
Solenostemon scutellarioides
Nat. Prod. Res.
23
127-137
2009
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700648
Xiao
Methyl jasmonate dramatically ...
Salvia miltiorrhiza
Physiol. Plant.
137
1-9
2009
1
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657023
Kim
Purification, cloning and func ...
Solenostemon scutellarioides
Plant Mol. Biol.
54
311-323
2004
-
-
1
-
-
-
-
-
1
-
3
1
-
5
-
-
1
-
-
1
1
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
3
1
-
-
-
1
-
1
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286351
Petersen
-
The biosynthesis of rosmarinic ...
Solenostemon scutellarioides
Plant Cell Tissue Organ Cult.
38
171 - 179
1994
-
-
-
-
-
-
4
4
-
-
-
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
4
-
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286352
Petersen
-
Proposed biosynthetic pathway ...
Solenostemon scutellarioides
Planta
189
10-14
1993
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286350
Husler
-
Hydroxyphenylpyruvate reductas ...
Solenostemon scutellarioides
Z. Naturforsch. C
46
371-376
1991
-
-
-
-
-
-
5
5
-
-
-
1
-
1
-
-
-
-
-
-
-
2
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
5
-
-
-
1
-
-
-
-
-
-
-
2
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286349
Petersen
-
Two new enzymes of rosmarinic ...
Solenostemon scutellarioides
Z. Naturforsch. C
43
501-504
1988
2
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
3
-
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-
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
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-
-