BRENDA - Enzyme Database show
show all sequences of 1.1.1.236

Two tropinone reducing enzymes from Datura stramonium transformed root cultures

Portsteffen, A.; Draeger, B.; Nahrstedt, A.; Phytochemistry 31, 1135-1138 (1992)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
activity rapidly lost during purification
Datura stramonium
activity rapidly lost during purification
Atropa belladonna
activity rapidly lost during purification
Hyoscyamus niger
Inhibitors
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoic acid
-
Hyoscyamus niger
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0061
-
NADPH
-
Hyoscyamus niger
0.0076
-
NADH
-
Hyoscyamus niger
0.017
-
NADPH
-
Datura stramonium
0.0211
-
NADPH
-
Hyoscyamus niger
0.023
-
NADPH
-
Datura stramonium
0.034
-
tropinone
-
Hyoscyamus niger
0.0351
-
tropinone
-
Hyoscyamus niger
0.22
-
tropinone
-
Datura stramonium
0.251
-
NADP+
-
Hyoscyamus niger
0.265
-
N-propylyl-4-piperidone
-
Hyoscyamus niger
0.534
-
4-ethylcyclohexanone
-
Hyoscyamus niger
0.77
-
N-Methyl-4-piperidone
-
Hyoscyamus niger
0.83
-
tropinone
-
Datura stramonium
2.03
-
4-methylcyclohexanone
-
Hyoscyamus niger
7.58
-
3-methylcyclohexanone
-
Hyoscyamus niger
21.6
-
4-Piperidone
-
Hyoscyamus niger
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
55000
TRII preparation is not yet pure and shows numerous bands
Datura stramonium
29000
-
3 * 29000, SDS-PAGE; 4 * 29000, SDS-PAGE
Hyoscyamus niger
69000
-
minor peak, gel filtration
Hyoscyamus niger
103000
-
major peak, gel filtration
Hyoscyamus niger
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tropinone + NADPH + H+
Datura inoxia
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Datura stramonium
-
pseudotropine + NADP+
-
-
-
tropinone + NADPH + H+
Atropa belladonna
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Hyoscyamus muticus
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Hyoscyamus pusillus
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Hyoscyamus canariensis
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Hyoscyamus bohemicus
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Physochlaina orientalis
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Physalis philadelphica
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Physalis edulis
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Calystegia sepium
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Physalis alkekengi
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Hyoscyamus niger
reverse reaction very inefficient, about 1.3% of the forward activity
pseudotropine + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Atropa belladonna
-
-
-
Calystegia sepium
-
-
-
Datura inoxia
-
-
-
Datura stramonium
-
-
-
Hyoscyamus bohemicus
-
-
-
Hyoscyamus canariensis
-
-
-
Hyoscyamus muticus
-
-
-
Hyoscyamus niger
-
-
-
Hyoscyamus pusillus
-
-
-
no activity in Brassica campestris
-
-
-
no activity in Browallia americana
-
-
-
no activity in Nicotiana tabacum
-
tobacco
-
Physalis alkekengi
-
-
-
Physalis edulis
-
-
-
Physalis philadelphica
-
-
-
Physochlaina orientalis
-
-
-
Purification (Commentary)
Commentary
Organism
-
Datura stramonium
-
Hyoscyamus niger
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Atropa belladonna
-
leaf
-
Atropa belladonna
-
root
-
Calystegia sepium
-
root
-
Datura inoxia
-
root
-
Physalis alkekengi
-
root
-
Physalis edulis
-
root
-
Physalis philadelphica
-
root
hairy roots
Datura stramonium
-
root
-
Atropa belladonna
-
root
-
Hyoscyamus niger
-
stem
-
Atropa belladonna
-
stem
-
Datura stramonium
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.79
-
-
Datura stramonium
Storage Stability
Storage Stability
Organism
-20°C, stable for at least 3 months
Datura stramonium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methyl-cyclohexanone + NADPH
-
348025
Hyoscyamus niger
2-methylcyclohexanol + NADP+
-
-
-
?
3-methyl-cyclohexanone + NADPH
-
348025
Hyoscyamus niger
3-methylcyclohexanol + NADP+
-
-
-
?
4-ethyl-cyclohexanone + NADPH
-
348025
Datura stramonium
4-ethylcyclohexanol + NADP+
-
-
-
?
4-ethyl-cyclohexanone + NADPH
-
348025
Hyoscyamus niger
4-ethylcyclohexanol + NADP+
-
-
-
?
4-methyl-cyclohexanone + NADPH
-
348025
Datura stramonium
4-methylcyclohexanol + NADP+
-
-
-
?
4-methyl-cyclohexanone + NADPH
-
348025
Hyoscyamus niger
4-methylcyclohexanol + NADP+
-
-
-
?
4-piperidone + NADPH
-
348025
Hyoscyamus niger
piperidine-4-ol + NADP+
-
-
-
?
6-hydroxytropinone + NADPH
-
348025
Datura stramonium
? + NADP+
-
-
-
?
6-hydroxytropinone + NADPH
-
348025
Hyoscyamus niger
? + NADP+
-
-
-
?
N-methyl-4-piperidone + NADPH
-
348025
Datura stramonium
N-methylpiperidin-4-ol + NADP+
-
-
-
?
N-methyl-4-piperidone + NADPH
-
348025
Hyoscyamus niger
N-methylpiperidin-4-ol + NADP+
-
-
-
?
N-propyl-4-piperidone + NADPH
-
348025
Datura stramonium
N-propylpiperidin-4-ol + NADP+
-
-
-
?
N-propyl-4-piperidone + NADPH
-
348025
Hyoscyamus niger
N-propylpiperidin-4-ol + NADP+
-
-
-
?
tetrahydrothiopyran-4-one + NADPH
-
348025
Datura stramonium
tetrahydrothiopyran-4-ol + NADP+
-
-
-
?
tetrahydrothiopyran-4-one + NADPH
-
348025
Hyoscyamus niger
tetrahydrothiopyran-4-ol + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Datura inoxia
pseudotropine + NADP+
-
-
-
-
tropinone + NADPH + H+
-
348025
Datura inoxia
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Datura stramonium
pseudotropine + NADP+
-
-
-
-
tropinone + NADPH + H+
-
348025
Atropa belladonna
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Hyoscyamus niger
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Hyoscyamus muticus
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Hyoscyamus pusillus
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Hyoscyamus canariensis
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Hyoscyamus bohemicus
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Physochlaina orientalis
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Physalis philadelphica
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Physalis edulis
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Calystegia sepium
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Physalis alkekengi
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
reverse reaction very inefficient, about 1.3% of the forward activity
348025
Hyoscyamus niger
pseudotropine + NADP+
-
-
-
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 29000, SDS-PAGE
Hyoscyamus niger
trimer
-
Atropa belladonna
trimer
-
Datura stramonium
trimer
3 * 29000, SDS-PAGE
Hyoscyamus niger
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3
6.5
-
Hyoscyamus niger
5.8
-
forward reaction
Hyoscyamus niger
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
5
reverse oxidative activities
Hyoscyamus niger
4.5
8.5
tolerant to pH changes over a wide range
Datura stramonium
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.8
8.5
more acidic buffers inactivate the enzyme highly, 8% of the maximum activity at pH 4.5, more alkaline buffers inhibit the activity less strongly, 61% of the maximum activity at pH 8.5
Hyoscyamus niger
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
activity exclusively with NADPH, not active with NADH
Datura stramonium
NADPH
-
Hyoscyamus niger
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
activity exclusively with NADPH, not active with NADH
Datura stramonium
NADPH
-
Hyoscyamus niger
General Stability (protein specific)
General Stability
Organism
activity rapidly lost during purification
Datura stramonium
activity rapidly lost during purification
Atropa belladonna
activity rapidly lost during purification
Hyoscyamus niger
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoic acid
-
Hyoscyamus niger
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0061
-
NADPH
-
Hyoscyamus niger
0.0076
-
NADH
-
Hyoscyamus niger
0.017
-
NADPH
-
Datura stramonium
0.0211
-
NADPH
-
Hyoscyamus niger
0.023
-
NADPH
-
Datura stramonium
0.034
-
tropinone
-
Hyoscyamus niger
0.0351
-
tropinone
-
Hyoscyamus niger
0.22
-
tropinone
-
Datura stramonium
0.251
-
NADP+
-
Hyoscyamus niger
0.265
-
N-propylyl-4-piperidone
-
Hyoscyamus niger
0.534
-
4-ethylcyclohexanone
-
Hyoscyamus niger
0.77
-
N-Methyl-4-piperidone
-
Hyoscyamus niger
0.83
-
tropinone
-
Datura stramonium
2.03
-
4-methylcyclohexanone
-
Hyoscyamus niger
7.58
-
3-methylcyclohexanone
-
Hyoscyamus niger
21.6
-
4-Piperidone
-
Hyoscyamus niger
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
55000
TRII preparation is not yet pure and shows numerous bands
Datura stramonium
29000
-
3 * 29000, SDS-PAGE; 4 * 29000, SDS-PAGE
Hyoscyamus niger
69000
-
minor peak, gel filtration
Hyoscyamus niger
103000
-
major peak, gel filtration
Hyoscyamus niger
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tropinone + NADPH + H+
Datura inoxia
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Datura stramonium
-
pseudotropine + NADP+
-
-
-
tropinone + NADPH + H+
Atropa belladonna
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Hyoscyamus muticus
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Hyoscyamus pusillus
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Hyoscyamus canariensis
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Hyoscyamus bohemicus
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Physochlaina orientalis
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Physalis philadelphica
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Physalis edulis
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Calystegia sepium
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Physalis alkekengi
-
pseudotropine + NADP+
-
-
?
tropinone + NADPH + H+
Hyoscyamus niger
reverse reaction very inefficient, about 1.3% of the forward activity
pseudotropine + NADP+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Datura stramonium
-
Hyoscyamus niger
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Atropa belladonna
-
leaf
-
Atropa belladonna
-
root
-
Calystegia sepium
-
root
-
Datura inoxia
-
root
-
Physalis alkekengi
-
root
-
Physalis edulis
-
root
-
Physalis philadelphica
-
root
hairy roots
Datura stramonium
-
root
-
Atropa belladonna
-
root
-
Hyoscyamus niger
-
stem
-
Atropa belladonna
-
stem
-
Datura stramonium
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.79
-
-
Datura stramonium
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for at least 3 months
Datura stramonium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methyl-cyclohexanone + NADPH
-
348025
Hyoscyamus niger
2-methylcyclohexanol + NADP+
-
-
-
?
3-methyl-cyclohexanone + NADPH
-
348025
Hyoscyamus niger
3-methylcyclohexanol + NADP+
-
-
-
?
4-ethyl-cyclohexanone + NADPH
-
348025
Datura stramonium
4-ethylcyclohexanol + NADP+
-
-
-
?
4-ethyl-cyclohexanone + NADPH
-
348025
Hyoscyamus niger
4-ethylcyclohexanol + NADP+
-
-
-
?
4-methyl-cyclohexanone + NADPH
-
348025
Datura stramonium
4-methylcyclohexanol + NADP+
-
-
-
?
4-methyl-cyclohexanone + NADPH
-
348025
Hyoscyamus niger
4-methylcyclohexanol + NADP+
-
-
-
?
4-piperidone + NADPH
-
348025
Hyoscyamus niger
piperidine-4-ol + NADP+
-
-
-
?
6-hydroxytropinone + NADPH
-
348025
Datura stramonium
? + NADP+
-
-
-
?
6-hydroxytropinone + NADPH
-
348025
Hyoscyamus niger
? + NADP+
-
-
-
?
N-methyl-4-piperidone + NADPH
-
348025
Datura stramonium
N-methylpiperidin-4-ol + NADP+
-
-
-
?
N-methyl-4-piperidone + NADPH
-
348025
Hyoscyamus niger
N-methylpiperidin-4-ol + NADP+
-
-
-
?
N-propyl-4-piperidone + NADPH
-
348025
Datura stramonium
N-propylpiperidin-4-ol + NADP+
-
-
-
?
N-propyl-4-piperidone + NADPH
-
348025
Hyoscyamus niger
N-propylpiperidin-4-ol + NADP+
-
-
-
?
tetrahydrothiopyran-4-one + NADPH
-
348025
Datura stramonium
tetrahydrothiopyran-4-ol + NADP+
-
-
-
?
tetrahydrothiopyran-4-one + NADPH
-
348025
Hyoscyamus niger
tetrahydrothiopyran-4-ol + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Datura inoxia
pseudotropine + NADP+
-
-
-
-
tropinone + NADPH + H+
-
348025
Datura inoxia
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Datura stramonium
pseudotropine + NADP+
-
-
-
-
tropinone + NADPH + H+
-
348025
Atropa belladonna
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Hyoscyamus niger
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Hyoscyamus muticus
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Hyoscyamus pusillus
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Hyoscyamus canariensis
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Hyoscyamus bohemicus
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Physochlaina orientalis
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Physalis philadelphica
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Physalis edulis
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Calystegia sepium
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
-
348025
Physalis alkekengi
pseudotropine + NADP+
-
-
-
?
tropinone + NADPH + H+
reverse reaction very inefficient, about 1.3% of the forward activity
348025
Hyoscyamus niger
pseudotropine + NADP+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 29000, SDS-PAGE
Hyoscyamus niger
trimer
-
Atropa belladonna
trimer
-
Datura stramonium
trimer
3 * 29000, SDS-PAGE
Hyoscyamus niger
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3
6.5
-
Hyoscyamus niger
5.8
-
forward reaction
Hyoscyamus niger
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
5
reverse oxidative activities
Hyoscyamus niger
4.5
8.5
tolerant to pH changes over a wide range
Datura stramonium
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.8
8.5
more acidic buffers inactivate the enzyme highly, 8% of the maximum activity at pH 4.5, more alkaline buffers inhibit the activity less strongly, 61% of the maximum activity at pH 8.5
Hyoscyamus niger
Other publictions for EC 1.1.1.236
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726209
Dehghan
An atypical pattern of accumul ...
Hyoscyamus muticus, Hyoscyamus senecionis
Plant Physiol. Biochem.
70C
188-194
2013
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
696874
Kai
Molecular cloning, characteriz ...
Anisodus acutangulus
Biotechnol. Appl. Biochem.
54
:177-186
2009
1
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
689548
Brock
The functional divergence of s ...
Cochlearia officinalis
Plant J.
54
388-401
2008
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689953
Freydank
Protein structure modeling ind ...
Solanum dulcamara
Proteins
72
173-183
2008
-
-
1
-
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
3
2
1
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
-
-
3
2
1
-
-
-
1
1
-
-
-
-
-
-
-
-
670518
Draeger
Tropinone reductases, enzymes ...
Atropa belladonna, Datura stramonium, Hyoscyamus niger, Solanum tuberosum
Phytochemistry
67
327-337
2006
-
-
4
-
-
-
-
18
-
-
4
-
-
4
-
-
-
-
-
4
4
-
14
-
-
-
-
1
4
-
-
4
-
-
-
-
-
4
4
-
-
-
-
-
-
18
-
-
4
-
-
-
-
-
-
4
4
-
14
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
670636
Kaiser
Immunolocalisation of two trop ...
Solanum tuberosum
Planta
225
127-137
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
4
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
656413
Richter
Overexpression of tropinone re ...
Datura stramonium
J. Exp. Bot.
56
645-652
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654707
Yamashita
Capturing enzyme structure pri ...
Datura stramonium
Biochemistry
42
5566-5573
2003
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
348033
Keiner
Molecular cloning, expression ...
Atropa belladonna, Datura stramonium, Hyoscyamus niger, Solanum tuberosum
Plant Mol. Biol.
48
299-308
2002
-
-
2
1
-
-
-
4
-
-
-
4
-
7
-
-
2
-
-
17
-
-
8
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
3
1
-
-
-
-
-
4
-
-
-
4
-
-
-
2
-
17
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347972
Nakajima
Site-directed mutagenesis of p ...
Arabidopsis sp., Datura stramonium, Hyoscyamus niger
J. Biol. Chem.
274
16563-16568
1999
-
-
3
1
-
-
-
19
-
-
-
2
-
3
-
-
2
-
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
19
-
-
-
2
-
-
-
2
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347973
Boswell
Specificities of the enzymes o ...
Brugmansia x candida, Datura stramonium, Datura stramonium D15/5, Hyoscyamus niger
Phytochemistry
52
871-878
1999
-
-
-
-
-
-
-
6
-
-
-
3
-
9
-
-
2
-
-
3
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
3
-
-
-
2
-
3
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348030
Nakajima
Two tropinone reductases, that ...
Atropa belladonna, Datura stramonium, Hyoscyamus niger, no activity in Nicotiana tabacum
Plant Cell Physiol.
40
1099-1107
1999
-
-
2
1
-
-
-
-
-
-
1
3
-
4
-
-
2
-
-
4
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
1
3
-
-
-
2
-
4
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348032
Yamashita
Structure of tropinone reducta ...
Datura stramonium
Biochemistry
38
7630-7637
1999
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348028
Nakajima
Crystal structures of two trop ...
Datura stramonium, Hyoscyamus niger
Proc. Natl. Acad. Sci. USA
95
4876-4881
1998
-
-
1
1
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348027
Portsteffen
The reduction of tropinone in ...
Atropa belladonna, Datura stramonium
Phytochemistry
37
391-400
1994
-
-
1
-
-
-
-
8
-
-
5
2
-
3
-
-
2
-
-
2
1
-
8
2
-
-
-
-
2
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
8
-
-
5
2
-
-
-
2
-
2
1
-
8
2
-
-
-
-
2
-
1
-
-
-
-
-
-
-
348026
Nakajima
Two tropinone reductases with ...
Atropa belladonna, Datura stramonium, Hyoscyamus niger, no activity in Nicotiana tabacum
Proc. Natl. Acad. Sci. USA
90
9591-9595
1993
-
-
2
-
-
-
-
-
-
-
-
3
-
8
-
-
1
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348023
Draeger
Levels of tropinone reductase ...
Atropa belladonna, Datura inoxia, Datura stramonium, Hyoscyamus niger, Physalis peruviana
Planta
188
581-586
1992
-
-
-
-
-
-
-
1
-
-
-
5
-
6
-
-
2
-
-
4
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
5
-
-
-
2
-
4
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348024
Hashimoto
Two tropinone reductases with ...
Hyoscyamus niger
Plant Physiol.
100
836-845
1992
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348025
Portsteffen
-
Two tropinone reducing enzymes ...
Atropa belladonna, Calystegia sepium, Datura inoxia, Datura stramonium, Hyoscyamus bohemicus, Hyoscyamus canariensis, Hyoscyamus muticus, Hyoscyamus niger, Hyoscyamus pusillus, no activity in Brassica campestris, no activity in Browallia americana, no activity in Nicotiana tabacum, Physalis alkekengi, Physalis edulis, Physalis philadelphica, Physochlaina orientalis
Phytochemistry
31
1135-1138
1992
-
-
-
-
-
3
1
16
-
-
4
13
-
16
-
-
2
-
-
12
1
1
30
4
-
-
-
-
2
2
1
2
-
-
-
-
-
-
2
-
-
3
-
1
-
16
-
-
4
13
-
-
-
2
-
12
1
1
30
4
-
-
-
-
2
2
1
-
-
-
-
-
-
-
348022
Couladis
-
Enzymes catalysing the reducti ...
Atropa belladonna, Datura inoxia, Datura stramonium, Hyoscyamus niger
Phytochemistry
30
801-805
1991
-
-
-
-
-
2
-
-
-
-
-
4
-
4
-
-
2
-
-
4
1
-
14
-
1
-
-
-
4
-
-
6
-
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
-
4
-
-
-
2
-
4
1
-
14
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
348021
Draeger
-
Purification and characterizat ...
Datura stramonium, Hyoscyamus niger
Agric. Biol. Chem.
52
2663-2667
1988
-
-
-
-
-
-
-
4
-
-
2
2
-
2
-
-
1
-
-
2
1
2
15
2
1
-
1
-
1
-
1
4
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
2
2
-
-
-
1
-
2
1
2
15
2
1
-
1
-
1
-
1
-
-
-
-
-
-
-