BRENDA - Enzyme Database show
show all sequences of 1.1.1.235

8-Ketodeoxycoformycin and 8-ketocoformycin as intermediates in the biosynthesis of 2-deoxycoformycin and coformycin

Hanvey, J.C.; Hawkins, E.S.; Baker, D.C.; Suhadolnik, R.J.; Biochemistry 27, 5790-5795 (1988)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
8-Ketocoformycin
-
Streptomyces antibioticus
0.25
-
8-Ketodeoxycoformycin
-
Streptomyces antibioticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
8-ketocoformycin + NADPH
Streptomyces antibioticus
production of the antibiotic coformycin
coformycin + NADP+
-
Streptomyces antibioticus
r
8-ketodeoxycoformycin + NADPH
Streptomyces antibioticus
production of the antibiotic 2'-deoxycoformycin
2'-deoxycoformycin + NADP+
-
Streptomyces antibioticus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces antibioticus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Streptomyces antibioticus
Reaction
Reaction
Commentary
Organism
coformycin + NADP+ = 8-oxocoformycin + NADPH + H+
possible mechanism
Streptomyces antibioticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
8-ketocoformycin + NADPH
stereospecifically reduces the 8-keto group of the ketonucleoside substrate to a hydroxyl group with the R configuration at C-8
286348
Streptomyces antibioticus
coformycin + NADP+
-
286348
Streptomyces antibioticus
r
8-ketocoformycin + NADPH
production of the antibiotic coformycin
286348
Streptomyces antibioticus
coformycin + NADP+
-
286348
Streptomyces antibioticus
r
8-ketodeoxycoformycin + NADPH
stereospecifically reduces the 8-keto group of the ketonucleoside substrate to a hydroxyl group with the R configuration at C-8
286348
Streptomyces antibioticus
2'-deoxycoformycin + NADP+
-
286348
Streptomyces antibioticus
r
8-ketodeoxycoformycin + NADPH
production of the antibiotic 2'-deoxycoformycin
286348
Streptomyces antibioticus
2'-deoxycoformycin + NADP+
-
286348
Streptomyces antibioticus
r
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Streptomyces antibioticus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
55
-
no activity
Streptomyces antibioticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Streptomyces antibioticus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADPH is 25-fold higher active than NADH
Streptomyces antibioticus
NADPH
B-specific with respect to NADPH, 25-fold higher activity than with NADH
Streptomyces antibioticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADPH is 25-fold higher active than NADH
Streptomyces antibioticus
NADPH
B-specific with respect to NADPH, 25-fold higher activity than with NADH
Streptomyces antibioticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
8-Ketocoformycin
-
Streptomyces antibioticus
0.25
-
8-Ketodeoxycoformycin
-
Streptomyces antibioticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
8-ketocoformycin + NADPH
Streptomyces antibioticus
production of the antibiotic coformycin
coformycin + NADP+
-
Streptomyces antibioticus
r
8-ketodeoxycoformycin + NADPH
Streptomyces antibioticus
production of the antibiotic 2'-deoxycoformycin
2'-deoxycoformycin + NADP+
-
Streptomyces antibioticus
r
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Streptomyces antibioticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
8-ketocoformycin + NADPH
stereospecifically reduces the 8-keto group of the ketonucleoside substrate to a hydroxyl group with the R configuration at C-8
286348
Streptomyces antibioticus
coformycin + NADP+
-
286348
Streptomyces antibioticus
r
8-ketocoformycin + NADPH
production of the antibiotic coformycin
286348
Streptomyces antibioticus
coformycin + NADP+
-
286348
Streptomyces antibioticus
r
8-ketodeoxycoformycin + NADPH
stereospecifically reduces the 8-keto group of the ketonucleoside substrate to a hydroxyl group with the R configuration at C-8
286348
Streptomyces antibioticus
2'-deoxycoformycin + NADP+
-
286348
Streptomyces antibioticus
r
8-ketodeoxycoformycin + NADPH
production of the antibiotic 2'-deoxycoformycin
286348
Streptomyces antibioticus
2'-deoxycoformycin + NADP+
-
286348
Streptomyces antibioticus
r
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Streptomyces antibioticus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
55
-
no activity
Streptomyces antibioticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Streptomyces antibioticus
Other publictions for EC 1.1.1.235
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286348
Hanvey
8-Ketodeoxycoformycin and 8-ke ...
Streptomyces antibioticus
Biochemistry
27
5790-5795
1988
-
-
-
-
-
-
-
2
-
-
-
2
-
1
-
-
1
1
-
-
-
-
4
-
1
1
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
-
4
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-