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show all sequences of 1.1.1.234

Molecular characterization and expression analysis of dihydroflavonol 4-reductase (DFR) gene in Saussurea medusa

Li, H.; Qiu, J.; Chen, F.; Lv, X.; Fu, C.; Zhao, D.; Hua, X.; Zhao, Q.; Mol. Biol. Rep. 39, 2991-2999 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene SmDFR, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, functional expression in Saccharomyces cerevisiae strain INV Sc1
Saussurea medusa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Saussurea medusa
the enzyme also catalyzes the reduction of dihydroflavonols to leucoanthocyanins, SmDFR can readily reduce dihydroquercetin and dihydrokampferol, as a dihydroflavonol 4-reductase, DFR, EC 1.1.1.219, but it cannot reduce dihydromyricetin
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saussurea medusa
A5Z0G1
gene SmDFR
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
callus
a red callus line
Saussurea medusa
-
flower
-
Saussurea medusa
-
leaf
-
Saussurea medusa
-
additional information
expression level of SmDFR is higher in flowers compared with both leaves and roots
Saussurea medusa
-
root
-
Saussurea medusa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-eriodictyol + NADPH + H+
-
725929
Saussurea medusa
luteoforol + NADP+
-
-
-
?
additional information
the enzyme also catalyzes the reduction of dihydroflavonols to leucoanthocyanins, SmDFR can readily reduce dihydroquercetin and dihydrokampferol, as a dihydroflavonol 4-reductase, DFR, EC 1.1.1.219, but it cannot reduce dihydromyricetin
725929
Saussurea medusa
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Saussurea medusa
Cloned(Commentary) (protein specific)
Commentary
Organism
gene SmDFR, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, functional expression in Saccharomyces cerevisiae strain INV Sc1
Saussurea medusa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Saussurea medusa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Saussurea medusa
the enzyme also catalyzes the reduction of dihydroflavonols to leucoanthocyanins, SmDFR can readily reduce dihydroquercetin and dihydrokampferol, as a dihydroflavonol 4-reductase, DFR, EC 1.1.1.219, but it cannot reduce dihydromyricetin
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
callus
a red callus line
Saussurea medusa
-
flower
-
Saussurea medusa
-
leaf
-
Saussurea medusa
-
additional information
expression level of SmDFR is higher in flowers compared with both leaves and roots
Saussurea medusa
-
root
-
Saussurea medusa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-eriodictyol + NADPH + H+
-
725929
Saussurea medusa
luteoforol + NADP+
-
-
-
?
additional information
the enzyme also catalyzes the reduction of dihydroflavonols to leucoanthocyanins, SmDFR can readily reduce dihydroquercetin and dihydrokampferol, as a dihydroflavonol 4-reductase, DFR, EC 1.1.1.219, but it cannot reduce dihydromyricetin
725929
Saussurea medusa
?
-
-
-
-
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the plant DFR superfamily, phylogenetic analysis
Saussurea medusa
metabolism
the DFR gene is a key gene late in the flavonoid biosynthesis pathway, overview. The enzyme posesses flavanone 4-reductase activity and also is a key enzyme in the biosynthesis of anthocyanidins, proanthocyanidins, and other flavonoids
Saussurea medusa
physiological function
the enzyme is of importance in plant development
Saussurea medusa
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the plant DFR superfamily, phylogenetic analysis
Saussurea medusa
metabolism
the DFR gene is a key gene late in the flavonoid biosynthesis pathway, overview. The enzyme posesses flavanone 4-reductase activity and also is a key enzyme in the biosynthesis of anthocyanidins, proanthocyanidins, and other flavonoids
Saussurea medusa
physiological function
the enzyme is of importance in plant development
Saussurea medusa
Other publictions for EC 1.1.1.234
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725929
Li
Molecular characterization and ...
Saussurea medusa
Mol. Biol. Rep.
39
2991-2999
2012
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3
3
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654357
Fischer
Molecular cloning, substrate s ...
Malus domestica, Pyrus communis
Arch. Biochem. Biophys.
412
223-230
2003
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4
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657102
Halbwirth
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Biochemical formation of antho ...
Zea mays
Plant Sci.
164
489-495
2003
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286346
Stich
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Biosynthesis of 3-deoxyanthocy ...
Sinningia cardinalis
Biochemistry
27
785-789
1988
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438999
Stich
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Studies on columnidin biosynth ...
Columnea hybrida, Columnea hybrida Heklua
Z. Naturforsch. C
43
311-314
1988
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