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Literature summary for 1.1.1.234 extracted from

  • Li, H.; Qiu, J.; Chen, F.; Lv, X.; Fu, C.; Zhao, D.; Hua, X.; Zhao, Q.
    Molecular characterization and expression analysis of dihydroflavonol 4-reductase (DFR) gene in Saussurea medusa (2012), Mol. Biol. Rep., 39, 2991-2999.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene SmDFR, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, functional expression in Saccharomyces cerevisiae strain INV Sc1 Saussurea medusa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saussurea medusa the enzyme also catalyzes the reduction of dihydroflavonols to leucoanthocyanins, SmDFR can readily reduce dihydroquercetin and dihydrokampferol, as a dihydroflavonol 4-reductase, DFR, EC 1.1.1.219, but it cannot reduce dihydromyricetin ?
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 ?

Organism

Organism UniProt Comment Textmining
Saussurea medusa A5Z0G1 gene SmDFR
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Source Tissue

Source Tissue Comment Organism Textmining
callus a red callus line Saussurea medusa
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flower
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 Saussurea medusa
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leaf
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 Saussurea medusa
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additional information expression level of SmDFR is higher in flowers compared with both leaves and roots Saussurea medusa
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root
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 Saussurea medusa
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-eriodictyol + NADPH + H+
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 Saussurea medusa luteoforol + NADP+
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 ?
additional information the enzyme also catalyzes the reduction of dihydroflavonols to leucoanthocyanins, SmDFR can readily reduce dihydroquercetin and dihydrokampferol, as a dihydroflavonol 4-reductase, DFR, EC 1.1.1.219, but it cannot reduce dihydromyricetin Saussurea medusa ?
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 ?

Cofactor

Cofactor Comment Organism Structure
NADPH
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 Saussurea medusa

General Information

General Information Comment Organism
evolution the enzyme belongs to the plant DFR superfamily, phylogenetic analysis Saussurea medusa
metabolism the DFR gene is a key gene late in the flavonoid biosynthesis pathway, overview. The enzyme posesses flavanone 4-reductase activity and also is a key enzyme in the biosynthesis of anthocyanidins, proanthocyanidins, and other flavonoids Saussurea medusa
physiological function the enzyme is of importance in plant development Saussurea medusa