BRENDA - Enzyme Database show
show all sequences of 1.1.1.234

Biosynthesis of 3-deoxyanthocyanins with flower extracts from Sinningia cardinalis

Stich, K.; Forkmann, G.; Biochemistry 27, 785-789 (1988)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
freezing fresh flowers in liquid nitrogen and storing them at -80░C enhances extractable activity compared to fresh flowers
Sinningia cardinalis
Inhibitors
Inhibitors
Commentary
Organism
Structure
glycerol
10% v/v, complete irreversible inactivation
Sinningia cardinalis
additional information
no inhibition by KCN, EDTA, diethyl dithiocarbamate, phenanthroline or N-ethylmaleimide, 2 mM each
Sinningia cardinalis
naringenin
above 0.1 mM
Sinningia cardinalis
p-chloromercuribenzoate
0.5 mM
Sinningia cardinalis
Sucrose
20% w/v, complete irreversible inactivation
Sinningia cardinalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
0.023
(2S)-naringenin
-
Sinningia cardinalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S)-flavanone + NADPH
Sinningia cardinalis
involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol
(2S)-flavan-4-ol + NADP+
-
Sinningia cardinalis
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sinningia cardinalis
-
syn. Rechsteineria cardinalis, strain ' Feuerschein'
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Sinningia cardinalis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.015
-
-
Sinningia cardinalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-eriodictyol + NADPH
-
286346
Sinningia cardinalis
luteoforol + NADP+
-
286346
Sinningia cardinalis
?
(2S)-flavanone + NADPH
involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol
286346
Sinningia cardinalis
(2S)-flavan-4-ol + NADP+
-
286346
Sinningia cardinalis
?
(2S)-naringenin + NADPH + H+
-
286346
Sinningia cardinalis
apiforol + NADP+
-
286346
Sinningia cardinalis
?
5,7,3',4',5'-pentahydroxyflavanone + NADPH
-
286346
Sinningia cardinalis
5,7,3',4',5'-pentahydroxyflavan-4-ol + NADP+
-
286346
Sinningia cardinalis
?
additional information
as dihydroxyflavonols, such as dihydrokaempferol, dihydroquercetin and dihydromyricetin are substrates for the Sinningia enzyme preparation, too, the enzyme might be identical with dihydroflavonol-4-reductase
286346
Sinningia cardinalis
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Sinningia cardinalis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.7
6.9
about half-maximal activity at pH 4.7 and 6.9
Sinningia cardinalis
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactors are FAD, FMN or tetrahydropterin, with or without NADPH
Sinningia cardinalis
NADH
77% as active as NADPH
Sinningia cardinalis
NADPH
required
Sinningia cardinalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactors are FAD, FMN or tetrahydropterin, with or without NADPH
Sinningia cardinalis
NADH
77% as active as NADPH
Sinningia cardinalis
NADPH
required
Sinningia cardinalis
General Stability (protein specific)
General Stability
Organism
freezing fresh flowers in liquid nitrogen and storing them at -80░C enhances extractable activity compared to fresh flowers
Sinningia cardinalis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
glycerol
10% v/v, complete irreversible inactivation
Sinningia cardinalis
additional information
no inhibition by KCN, EDTA, diethyl dithiocarbamate, phenanthroline or N-ethylmaleimide, 2 mM each
Sinningia cardinalis
naringenin
above 0.1 mM
Sinningia cardinalis
p-chloromercuribenzoate
0.5 mM
Sinningia cardinalis
Sucrose
20% w/v, complete irreversible inactivation
Sinningia cardinalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
0.023
(2S)-naringenin
-
Sinningia cardinalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S)-flavanone + NADPH
Sinningia cardinalis
involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol
(2S)-flavan-4-ol + NADP+
-
Sinningia cardinalis
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Sinningia cardinalis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.015
-
-
Sinningia cardinalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-eriodictyol + NADPH
-
286346
Sinningia cardinalis
luteoforol + NADP+
-
286346
Sinningia cardinalis
?
(2S)-flavanone + NADPH
involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol
286346
Sinningia cardinalis
(2S)-flavan-4-ol + NADP+
-
286346
Sinningia cardinalis
?
(2S)-naringenin + NADPH + H+
-
286346
Sinningia cardinalis
apiforol + NADP+
-
286346
Sinningia cardinalis
?
5,7,3',4',5'-pentahydroxyflavanone + NADPH
-
286346
Sinningia cardinalis
5,7,3',4',5'-pentahydroxyflavan-4-ol + NADP+
-
286346
Sinningia cardinalis
?
additional information
as dihydroxyflavonols, such as dihydrokaempferol, dihydroquercetin and dihydromyricetin are substrates for the Sinningia enzyme preparation, too, the enzyme might be identical with dihydroflavonol-4-reductase
286346
Sinningia cardinalis
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Sinningia cardinalis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.7
6.9
about half-maximal activity at pH 4.7 and 6.9
Sinningia cardinalis
Other publictions for EC 1.1.1.234
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725929
Li
Molecular characterization and ...
Saussurea medusa
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2991-2999
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-
1
-
-
-
-
-
-
-
-
1
-
6
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
654357
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Malus domestica, Pyrus communis
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412
223-230
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-
-
1
-
-
-
-
-
-
-
-
6
-
7
-
-
-
2
-
2
-
-
10
-
4
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
2
-
-
10
-
4
-
-
-
2
-
-
-
-
-
-
-
-
-
657102
Halbwirth
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Biochemical formation of antho ...
Zea mays
Plant Sci.
164
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2003
-
-
1
-
-
-
2
-
-
-
-
3
-
1
-
-
-
1
-
1
-
-
7
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
1
-
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286346
Stich
-
Biosynthesis of 3-deoxyanthocy ...
Sinningia cardinalis
Biochemistry
27
785-789
1988
-
-
-
-
-
1
5
1
-
-
-
1
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
1
-
5
-
1
-
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
438999
Stich
-
Studies on columnidin biosynth ...
Columnea hybrida, Columnea hybrida Heklua
Z. Naturforsch. C
43
311-314
1988
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
1
-
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-