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show all sequences of 1.1.1.233

Cloning, sequencing, and expression of the N-acyl-D-mannosamine dehydrogenase gene from Flavobacterium sp. strain 141-8 in Escherichia coli

Yamamoto-Otake, H.; Koyama, Y.; Horiuchi, T.; Nakano, E.; Appl. Environ. Microbiol. 57, 1418-1422 (1991)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
NAD+
value unchanged by cloning and expression in E. coli JM109
Flavobacterium sp.
1
-
N-acetyl-D-mannosamine
value unchanged by cloning and expression in E. coli JM109
Flavobacterium sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-acetyl-D-mannosamine + NAD+
Flavobacterium sp.
-
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Flavobacterium sp.
-
-
-
Purification (Commentary)
Commentary
Organism
purified from Escherichia coli clone
Flavobacterium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
545
-
purified enzyme
Flavobacterium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-D-mannosamine + NAD+
-
389506
Flavobacterium sp.
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
-
N-acetyl-D-mannosamine + NAD+
-
389506
Flavobacterium sp.
N-acetyl-D-mannosaminolactone + NADH + H+
-
389506
Flavobacterium sp.
-
N-glycolyl-D-mannosamine + NAD+
oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation
389506
Flavobacterium sp.
N-glycolyl-D-mannosaminolactone + NADH
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
recombinant enzyme
Flavobacterium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
NAD+
value unchanged by cloning and expression in E. coli JM109
Flavobacterium sp.
1
-
N-acetyl-D-mannosamine
value unchanged by cloning and expression in E. coli JM109
Flavobacterium sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-acetyl-D-mannosamine + NAD+
Flavobacterium sp.
-
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
purified from Escherichia coli clone
Flavobacterium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
545
-
purified enzyme
Flavobacterium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-D-mannosamine + NAD+
-
389506
Flavobacterium sp.
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
-
N-acetyl-D-mannosamine + NAD+
-
389506
Flavobacterium sp.
N-acetyl-D-mannosaminolactone + NADH + H+
-
389506
Flavobacterium sp.
-
N-glycolyl-D-mannosamine + NAD+
oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation
389506
Flavobacterium sp.
N-glycolyl-D-mannosaminolactone + NADH
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
recombinant enzyme
Flavobacterium sp.
Other publictions for EC 1.1.1.233
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
389506
Yamamoto-Otake
Cloning, sequencing, and expre ...
Flavobacterium sp.
Appl. Environ. Microbiol.
57
1418-1422
1991
-
-
-
-
-
-
-
2
-
-
-
1
-
5
-
-
1
-
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389505
Horiuchi
Purification and properties of ...
Flavobacterium sp. 141-8, Flavobacterium sp.
J. Biochem.
104
466-471
1988
-
1
1
-
-
1
9
3
-
-
3
2
-
3
-
-
1
-
-
-
2
1
6
1
-
-
4
-
1
1
3
2
-
-
-
-
1
1
2
-
-
1
-
9
-
3
-
-
3
2
-
-
-
1
-
-
2
1
6
1
-
-
4
-
1
1
3
-
-
-
-
-
-
-