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show all sequences of 1.1.1.233

Purification and properties of N-acyl-D-mannosamine dehydrogenase from Flavobacterium sp. 141-8

Horiuchi, T.; Kurokawa, T.; J. Biochem. 104, 466-471 (1988)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
quantitative determination of N-acetylneuraminic acid
Flavobacterium sp.
Cloned(Commentary)
Commentary
Organism
cloned and expressed under control of a lac-promoter in Escherichia coli JM109, the Escherichia coli transformants JM109(pNAM307) and JM109(pNAM308) show up to 200-fold higher activity than Flavobacterium sp.
Flavobacterium sp.
General Stability
General Stability
Organism
inactivated by frequent freezing and thawing
Flavobacterium sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
AMP
slight inhibition
Flavobacterium sp.
Cd2+
slight inhibition
Flavobacterium sp.
Cu2+
slight inhibition
Flavobacterium sp.
Hg2+
strong inhibitor
Flavobacterium sp.
Mn2+
slight inhibition
Flavobacterium sp.
additional information
no inhibition with metal-chelating or SH-group-blocking reagents, e.g. EDTA, 2,2'-bipyridyl, 8-hydroxyquinoline, PCMB
Flavobacterium sp.
Ni2+
slight inhibition
Flavobacterium sp.
SDS
strong inhibitor
Flavobacterium sp.
Zn2+
slight inhibition
Flavobacterium sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
NAD+
-
Flavobacterium sp.
1
-
N-acetyl-D-mannosamine
-
Flavobacterium sp.
13.3
-
N-Glycolyl-D-mannosamine
-
Flavobacterium sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27470
-
calculated from amino acid sequence
Flavobacterium sp.
29000
-
SDS-PAGE
Flavobacterium sp.
120000
-
gel filtration
Flavobacterium sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-acetyl-D-mannosamine + NAD+
Flavobacterium sp.
reaction in N-acetyl-D-mannosamine catabolism
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
N-acetyl-D-mannosamine + NAD+
Flavobacterium sp. 141-8
reaction in N-acetyl-D-mannosamine catabolism
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Flavobacterium sp.
-
141-8
-
Flavobacterium sp. 141-8
-
141-8
-
Purification (Commentary)
Commentary
Organism
-
Flavobacterium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.13
-
crude extract
Flavobacterium sp.
340
-
purified enzyme
Flavobacterium sp.
Storage Stability
Storage Stability
Organism
-20°C, stable for at least several months
Flavobacterium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-D-mannosamine + NAD+
-
389505
Flavobacterium sp.
N-acetyl-D-mannosaminolactone + NADH + H+
-
389505
Flavobacterium sp.
ir
N-acetyl-D-mannosamine + NAD+
reaction in N-acetyl-D-mannosamine catabolism
389505
Flavobacterium sp.
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
-
N-acetyl-D-mannosamine + NAD+
-
389505
Flavobacterium sp. 141-8
N-acetyl-D-mannosaminolactone + NADH + H+
-
389505
Flavobacterium sp. 141-8
ir
N-acetyl-D-mannosamine + NAD+
reaction in N-acetyl-D-mannosamine catabolism
389505
Flavobacterium sp. 141-8
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
-
N-glycolyl-D-mannosamine + NAD+
oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation
389505
Flavobacterium sp.
N-glycolyl-D-mannosaminolactone + NADH
-
-
-
-
N-glycolyl-D-mannosamine + NAD+
oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation
389505
Flavobacterium sp. 141-8
N-glycolyl-D-mannosaminolactone + NADH
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 29000
Flavobacterium sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
inactivation at pH 5.0 after 16 h, gradually reversed to about 80% of original activity by shifting the pH back to 8.2 and keeping it at room temperature for 36 h
Flavobacterium sp.
42
-
and below, stable for at least 10 min
Flavobacterium sp.
50
-
t1/2: 10 min
Flavobacterium sp.
60
-
complete inactivation after 10 min
Flavobacterium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9.5
-
Flavobacterium sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
11.5
-
Flavobacterium sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
-
inactivation at 25°C after 16 h, gradually reversed to about 80% of original activity by shifting the pH back to 8.2 and keeping it at room temperature for 36 h
Flavobacterium sp.
7
-
and below, more than 70% loss of activity
Flavobacterium sp.
8.5
9.5
more stable than at pH 8.2 at 45°C, 10 min
Flavobacterium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADP+, ferricyanide, 2,6-dichlorophenolindophenol, phenazine methosulfate
Flavobacterium sp.
NAD+
specific for
Flavobacterium sp.
Application (protein specific)
Application
Commentary
Organism
analysis
quantitative determination of N-acetylneuraminic acid
Flavobacterium sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
cloned and expressed under control of a lac-promoter in Escherichia coli JM109, the Escherichia coli transformants JM109(pNAM307) and JM109(pNAM308) show up to 200-fold higher activity than Flavobacterium sp.
Flavobacterium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADP+, ferricyanide, 2,6-dichlorophenolindophenol, phenazine methosulfate
Flavobacterium sp.
NAD+
specific for
Flavobacterium sp.
General Stability (protein specific)
General Stability
Organism
inactivated by frequent freezing and thawing
Flavobacterium sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
AMP
slight inhibition
Flavobacterium sp.
Cd2+
slight inhibition
Flavobacterium sp.
Cu2+
slight inhibition
Flavobacterium sp.
Hg2+
strong inhibitor
Flavobacterium sp.
Mn2+
slight inhibition
Flavobacterium sp.
additional information
no inhibition with metal-chelating or SH-group-blocking reagents, e.g. EDTA, 2,2'-bipyridyl, 8-hydroxyquinoline, PCMB
Flavobacterium sp.
Ni2+
slight inhibition
Flavobacterium sp.
SDS
strong inhibitor
Flavobacterium sp.
Zn2+
slight inhibition
Flavobacterium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
NAD+
-
Flavobacterium sp.
1
-
N-acetyl-D-mannosamine
-
Flavobacterium sp.
13.3
-
N-Glycolyl-D-mannosamine
-
Flavobacterium sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27470
-
calculated from amino acid sequence
Flavobacterium sp.
29000
-
SDS-PAGE
Flavobacterium sp.
120000
-
gel filtration
Flavobacterium sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-acetyl-D-mannosamine + NAD+
Flavobacterium sp.
reaction in N-acetyl-D-mannosamine catabolism
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
N-acetyl-D-mannosamine + NAD+
Flavobacterium sp. 141-8
reaction in N-acetyl-D-mannosamine catabolism
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Flavobacterium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.13
-
crude extract
Flavobacterium sp.
340
-
purified enzyme
Flavobacterium sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for at least several months
Flavobacterium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-D-mannosamine + NAD+
-
389505
Flavobacterium sp.
N-acetyl-D-mannosaminolactone + NADH + H+
-
389505
Flavobacterium sp.
ir
N-acetyl-D-mannosamine + NAD+
reaction in N-acetyl-D-mannosamine catabolism
389505
Flavobacterium sp.
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
-
N-acetyl-D-mannosamine + NAD+
-
389505
Flavobacterium sp. 141-8
N-acetyl-D-mannosaminolactone + NADH + H+
-
389505
Flavobacterium sp. 141-8
ir
N-acetyl-D-mannosamine + NAD+
reaction in N-acetyl-D-mannosamine catabolism
389505
Flavobacterium sp. 141-8
N-acetyl-D-mannosaminolactone + NADH + H+
-
-
-
-
N-glycolyl-D-mannosamine + NAD+
oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation
389505
Flavobacterium sp.
N-glycolyl-D-mannosaminolactone + NADH
-
-
-
-
N-glycolyl-D-mannosamine + NAD+
oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation
389505
Flavobacterium sp. 141-8
N-glycolyl-D-mannosaminolactone + NADH
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 29000
Flavobacterium sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
inactivation at pH 5.0 after 16 h, gradually reversed to about 80% of original activity by shifting the pH back to 8.2 and keeping it at room temperature for 36 h
Flavobacterium sp.
42
-
and below, stable for at least 10 min
Flavobacterium sp.
50
-
t1/2: 10 min
Flavobacterium sp.
60
-
complete inactivation after 10 min
Flavobacterium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9.5
-
Flavobacterium sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
11.5
-
Flavobacterium sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
-
inactivation at 25°C after 16 h, gradually reversed to about 80% of original activity by shifting the pH back to 8.2 and keeping it at room temperature for 36 h
Flavobacterium sp.
7
-
and below, more than 70% loss of activity
Flavobacterium sp.
8.5
9.5
more stable than at pH 8.2 at 45°C, 10 min
Flavobacterium sp.
Other publictions for EC 1.1.1.233
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
389506
Yamamoto-Otake
Cloning, sequencing, and expre ...
Flavobacterium sp.
Appl. Environ. Microbiol.
57
1418-1422
1991
-
-
-
-
-
-
-
2
-
-
-
1
-
5
-
-
1
-
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389505
Horiuchi
Purification and properties of ...
Flavobacterium sp. 141-8, Flavobacterium sp.
J. Biochem.
104
466-471
1988
-
1
1
-
-
1
9
3
-
-
3
2
-
3
-
-
1
-
-
-
2
1
6
1
-
-
4
-
1
1
3
2
-
-
-
-
1
1
2
-
-
1
-
9
-
3
-
-
3
2
-
-
-
1
-
-
2
1
6
1
-
-
4
-
1
1
3
-
-
-
-
-
-
-