BRENDA - Enzyme Database show
show all sequences of 1.1.1.231

Isolation and properties of an NADP+-dependent PGI2-specific 15-hydroxyprostaglandin dehydrogenase from rabbit kidney

Korff, J.M.; Jarabak, J.; Methods Enzymol. 86, 152-155 (1982)

Data extracted from this reference:

General Stability
General Stability
Organism
freezing and thawing: repeatedly, slow loss of activity
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0134
-
NADP+
-
Oryctolagus cuniculus
0.278
-
prostaglandin I2
-
Oryctolagus cuniculus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
Oryctolagus cuniculus
-
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
19.9
-
-
Oryctolagus cuniculus
Storage Stability
Storage Stability
Organism
stable for months when frozen
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
-
348012
Oryctolagus cuniculus
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
-
-
-
?
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
i.e. prostaglandin I2, prostacyclin
348012
Oryctolagus cuniculus
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
i.e. 15-keto prostaglandin I2
348012
Oryctolagus cuniculus
?
6-keto-prostaglandin F1alpha + NADP+
at 1.8% the reaction rate of prostaglandin I2
348012
Oryctolagus cuniculus
6,15-ketoprostaglandin F2alpha + NADPH
-
-
-
?
additional information
enzyme catalyzes reduction of 9-keto group in prostaglandin E2 at less than 1% the rate of prostaglandin I2 oxidation
348012
Oryctolagus cuniculus
?
-
-
-
-
prostaglandin B1 + NADP+
at 8% the reaction rate of prostaglandin I2
348012
Oryctolagus cuniculus
15-ketoprostaglandin B1 + NADPH + H+
-
-
-
?
prostaglandin E2 + NADP+
at 4% the reaction rate of prostaglandin I2
348012
Oryctolagus cuniculus
15-ketoprostaglandin E2 + NADPH + H+
-
-
-
?
prostaglandin F2alpha + NADP+
at 2.2% the reaction rate of prostaglandin I2
348012
Oryctolagus cuniculus
15-ketoprostaglandin F2alpha + NADPH + H+
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.3
-
assay at
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NAD+
Oryctolagus cuniculus
NADP+
-
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NAD+
Oryctolagus cuniculus
NADP+
-
Oryctolagus cuniculus
General Stability (protein specific)
General Stability
Organism
freezing and thawing: repeatedly, slow loss of activity
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0134
-
NADP+
-
Oryctolagus cuniculus
0.278
-
prostaglandin I2
-
Oryctolagus cuniculus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
Oryctolagus cuniculus
-
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
19.9
-
-
Oryctolagus cuniculus
Storage Stability (protein specific)
Storage Stability
Organism
stable for months when frozen
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
-
348012
Oryctolagus cuniculus
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
-
-
-
?
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
i.e. prostaglandin I2, prostacyclin
348012
Oryctolagus cuniculus
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
i.e. 15-keto prostaglandin I2
348012
Oryctolagus cuniculus
?
6-keto-prostaglandin F1alpha + NADP+
at 1.8% the reaction rate of prostaglandin I2
348012
Oryctolagus cuniculus
6,15-ketoprostaglandin F2alpha + NADPH
-
-
-
?
additional information
enzyme catalyzes reduction of 9-keto group in prostaglandin E2 at less than 1% the rate of prostaglandin I2 oxidation
348012
Oryctolagus cuniculus
?
-
-
-
-
prostaglandin B1 + NADP+
at 8% the reaction rate of prostaglandin I2
348012
Oryctolagus cuniculus
15-ketoprostaglandin B1 + NADPH + H+
-
-
-
?
prostaglandin E2 + NADP+
at 4% the reaction rate of prostaglandin I2
348012
Oryctolagus cuniculus
15-ketoprostaglandin E2 + NADPH + H+
-
-
-
?
prostaglandin F2alpha + NADP+
at 2.2% the reaction rate of prostaglandin I2
348012
Oryctolagus cuniculus
15-ketoprostaglandin F2alpha + NADPH + H+
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.3
-
assay at
Oryctolagus cuniculus
Other publictions for EC 1.1.1.231
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
348015
Fujimori
Expression of NADP(+)-Dependen ...
Macaca fascicularis
J. Biochem.
131
383-389
2002
-
-
1
-
-
-
-
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-
-
1
-
-
1
-
-
-
-
-
2
5
-
3
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-
-
-
-
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-
-
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1
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1
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2
5
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3
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-
348016
Terada
Characterization of multiple C ...
Cricetulus griseus
Chem. Biol. Interact.
130-132
847-861
2001
-
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1
-
-
-
-
-
-
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1
-
-
1
-
-
-
-
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3
-
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1
-
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-
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1
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-
-
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-
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3
-
-
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-
-
-
-
-
-
-
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-
-
-
347836
Krook
Three-dimensional model of NAD ...
Homo sapiens
FEBS Lett.
322
139-142
1993
-
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-
-
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1
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1
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1
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1
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1
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-
-
348018
Krook
Carboxyethyllysine in a protei ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
90
502-506
1993
-
-
-
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-
-
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1
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1
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1
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1
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1
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1
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1
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348017
Jarabak
Substrate specificity of three ...
Homo sapiens
Prostaglandins
26
849-868
1983
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1
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1
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1
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1
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1
-
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-
-
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-
-
-
-
-
-
-
-
348012
Korff
Isolation and properties of an ...
Oryctolagus cuniculus
Methods Enzymol.
86
152-155
1982
-
-
-
-
-
1
-
2
-
-
1
1
-
2
-
-
1
-
-
2
1
1
7
-
1
-
-
-
1
-
-
2
-
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-
-
-
-
2
-
-
1
-
-
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2
-
-
1
1
-
-
-
1
-
2
1
1
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
348013
Korff
The purification and character ...
Oryctolagus cuniculus
J. Biol. Chem.
257
2177-2181
1982
-
-
-
-
-
-
2
5
-
-
2
1
-
2
-
1
1
1
-
2
1
-
5
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
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-
-
2
-
5
-
-
2
1
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1
1
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2
1
-
5
1
1
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1
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