BRENDA - Enzyme Database show
show all sequences of 1.1.1.230

Purification and properties of 3alpha-hydroxyglycyrrhetinate dehydrogenase of Clostridium innocuum from human intestine

Akao, T.; Akao, T.; Hattori, M.; Namba, T.; Kobashi, K.; J. Biochem. 103, 504-507 (1988)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00027
-
NADP+
-
Clostridium innocuum
0.001
-
3-Ketoglycyrrhetic acid
-
Clostridium innocuum
0.002
-
3alpha-Hydroxyglycyrrhetic acid
-
Clostridium innocuum
0.024
-
NADPH
-
Clostridium innocuum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Clostridium innocuum
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
2 * 30000, SDS-PAGE
Clostridium innocuum
53000
-
gel filtration
Clostridium innocuum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha-hydroxyglycyrrhetic acid + NADP+
Clostridium innocuum
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group
3-ketoglycyrrhetic acid + NADPH
-
Clostridium innocuum
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium innocuum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Clostridium innocuum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha-hydroxy-18alpha-glycyrrhetic acid + NADP+
-
286341
Clostridium innocuum
3-keto-18alpha-glycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
3alpha-hydroxyglycyrrhetic acid + NADP+
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group, not accepted as substrates: 3beta-hydroxyglycyrrhetic acid, steroids or bile acids containing 3alpha-hydroxy or 3-keto group
286341
Clostridium innocuum
3-ketoglycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
3alpha-hydroxyglycyrrhetic acid + NADP+
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group
286341
Clostridium innocuum
3-ketoglycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 30000, SDS-PAGE
Clostridium innocuum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of 3-keto group
Clostridium innocuum
8
-
oxidation of 3alpha-hydroxy group
Clostridium innocuum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Clostridium innocuum
NADPH
-
Clostridium innocuum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Clostridium innocuum
NADPH
-
Clostridium innocuum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00027
-
NADP+
-
Clostridium innocuum
0.001
-
3-Ketoglycyrrhetic acid
-
Clostridium innocuum
0.002
-
3alpha-Hydroxyglycyrrhetic acid
-
Clostridium innocuum
0.024
-
NADPH
-
Clostridium innocuum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Clostridium innocuum
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
2 * 30000, SDS-PAGE
Clostridium innocuum
53000
-
gel filtration
Clostridium innocuum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha-hydroxyglycyrrhetic acid + NADP+
Clostridium innocuum
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group
3-ketoglycyrrhetic acid + NADPH
-
Clostridium innocuum
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium innocuum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha-hydroxy-18alpha-glycyrrhetic acid + NADP+
-
286341
Clostridium innocuum
3-keto-18alpha-glycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
3alpha-hydroxyglycyrrhetic acid + NADP+
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group, not accepted as substrates: 3beta-hydroxyglycyrrhetic acid, steroids or bile acids containing 3alpha-hydroxy or 3-keto group
286341
Clostridium innocuum
3-ketoglycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
3alpha-hydroxyglycyrrhetic acid + NADP+
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group
286341
Clostridium innocuum
3-ketoglycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 30000, SDS-PAGE
Clostridium innocuum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of 3-keto group
Clostridium innocuum
8
-
oxidation of 3alpha-hydroxy group
Clostridium innocuum
Other publictions for EC 1.1.1.230
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286344
Akao
Hasty effects on the metabolis ...
Clostridium innocuum
Biol. Pharm. Bull.
23
6-11
2000
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286343
Akao
Distribution of enzymes involv ...
Rattus norvegicus
Biol. Pharm. Bull.
21
1036-1044
1998
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286342
Akao
Localization of enzymes involv ...
gastrointestinal bacteria
Biol. Pharm. Bull.
20
122-126
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286341
Akao
Purification and properties of ...
Clostridium innocuum
J. Biochem.
103
504-507
1988
-
-
-
-
-
-
-
4
1
-
2
1
-
1
-
-
1
-
-
-
-
-
3
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
1
-
2
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-