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Literature summary for 1.1.1.23 extracted from

  • Andorn, N.; Aronovitch, J.
    Purification and properties of histidinol dehydrogenase from Escherichia coli B (1982), J. Gen. Microbiol., 128, 579-584.
    View publication on PubMed
Search for more literature for 1.1.1.23

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 0.5 mM, 100% inhibition, 0.05 mM 79% inhibition Escherichia coli
Urea 2.2 mM, 50% inhibition, reversible Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
 NAD+
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.5 mM, 165% increase Escherichia coli
Zn2+ 0.5 mM, 20% increase Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 alpha,alpha, 2 * 52000, SDS-PAGE Escherichia coli
91000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-histidinal + NAD+ + H2O Escherichia coli reaction intermediate L-histidine + NADH + H+
-
 ?
L-histidinol + 2 NAD+ + H2O Escherichia coli
-
 L-histidine + 2 NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
60°C, pH 5 treatment, ammonium sulfate precipitation, gel filtration, DEAE-cellulose, gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.32
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-histidinal + NAD+ + H2O
-
 Escherichia coli L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O reaction intermediate Escherichia coli L-histidine + NADH + H+
-
 ?
L-histidinol + 2 NAD+ + H2O
-
 Escherichia coli L-histidine + 2 NADH + 2 H+
-
 ?

Subunits

Subunits Comment Organism
homodimer alpha,alpha, 2 * 52000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 almost no loss of activity after 5 min at pH 7.5, histidinol, 2 mM and MnCl2, 1 mM, protect enzyme from heat inactivation Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Escherichia coli