BRENDA - Enzyme Database show
show all sequences of 1.1.1.227

Molecular cloning and functional characterization of borneol dehydrogenase from the glandular trichomes of Lavandula x intermedia

Sarker, L.S.; Galata, M.; Demissie, Z.A.; Mahmoud, S.S.; Arch. Biochem. Biophys. 528, 163-170 (2012)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
the gene encoding the borneol dhdrogenase is a target for metabolic engineering for improvement of essential oil production
Lavandula x intermedia
Cloned(Commentary)
Commentary
Organism
gene LiBDH, DNA and amino acid sequence determination and analysis, phylogenetic tree, gene expression is regulated in a tissue-specific manner, expression of the His-tagged enzyme in Escherichia coli strain Rosetta (DE3)pLysS
Lavandula x intermedia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics, overview
Lavandula x intermedia
0.053
-
(-)-borneol
pH 8.0, 30C, recombinant enzyme
Lavandula x intermedia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27500
-
x * 27500, about, sequence calculation
Lavandula x intermedia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-borneol + NAD+
Lavandula x intermedia
-
(-)-camphor + NADH + H+
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lavandula x intermedia
-
gene LiBDH
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3)pLysSby nickel affinity chromatography
Lavandula x intermedia
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
specifically in glandular trichomes of mature flowers
Lavandula x intermedia
-
additional information
the gene exppression is regulated in a tissue-specific manner
Lavandula x intermedia
-
trichome
-
Lavandula x intermedia
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-borneol + NAD+
-
724125
Lavandula x intermedia
(-)-camphor + NADH + H+
-
-
-
ir
additional information
no activity with other monoterpenes, e.g. alpha terpineol, 1,8-cineole, citronellol, linalool, lavandulol, nerol, geraniol, and perillyl alcohol, and sesquiterpene farnesol, or with menthol, overview
724125
Lavandula x intermedia
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 27500, about, sequence calculation
Lavandula x intermedia
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
32
-
-
Lavandula x intermedia
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0004
-
(-)-borneol
pH 8.0, 30C, recombinant enzyme
Lavandula x intermedia
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Lavandula x intermedia
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Lavandula x intermedia
NAD+
preferred cofactor
Lavandula x intermedia
Application (protein specific)
Application
Commentary
Organism
biotechnology
the gene encoding the borneol dhdrogenase is a target for metabolic engineering for improvement of essential oil production
Lavandula x intermedia
Cloned(Commentary) (protein specific)
Commentary
Organism
gene LiBDH, DNA and amino acid sequence determination and analysis, phylogenetic tree, gene expression is regulated in a tissue-specific manner, expression of the His-tagged enzyme in Escherichia coli strain Rosetta (DE3)pLysS
Lavandula x intermedia
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Lavandula x intermedia
NAD+
preferred cofactor
Lavandula x intermedia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics, overview
Lavandula x intermedia
0.053
-
(-)-borneol
pH 8.0, 30C, recombinant enzyme
Lavandula x intermedia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27500
-
x * 27500, about, sequence calculation
Lavandula x intermedia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-borneol + NAD+
Lavandula x intermedia
-
(-)-camphor + NADH + H+
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3)pLysSby nickel affinity chromatography
Lavandula x intermedia
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
specifically in glandular trichomes of mature flowers
Lavandula x intermedia
-
additional information
the gene exppression is regulated in a tissue-specific manner
Lavandula x intermedia
-
trichome
-
Lavandula x intermedia
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-borneol + NAD+
-
724125
Lavandula x intermedia
(-)-camphor + NADH + H+
-
-
-
ir
additional information
no activity with other monoterpenes, e.g. alpha terpineol, 1,8-cineole, citronellol, linalool, lavandulol, nerol, geraniol, and perillyl alcohol, and sesquiterpene farnesol, or with menthol, overview
724125
Lavandula x intermedia
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 27500, about, sequence calculation
Lavandula x intermedia
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
32
-
-
Lavandula x intermedia
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0004
-
(-)-borneol
pH 8.0, 30C, recombinant enzyme
Lavandula x intermedia
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Lavandula x intermedia
General Information
General Information
Commentary
Organism
evolution
the enzyme is a member of the SDR superfamily of enzymes
Lavandula x intermedia
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme is a member of the SDR superfamily of enzymes
Lavandula x intermedia
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0075
-
(-)-borneol
pH 8.0, 30C, recombinant enzyme
Lavandula x intermedia
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0075
-
(-)-borneol
pH 8.0, 30C, recombinant enzyme
Lavandula x intermedia
Other publictions for EC 1.1.1.227
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724125
Sarker
Molecular cloning and function ...
Lavandula x intermedia
Arch. Biochem. Biophys.
528
163-170
2012
-
1
1
-
-
-
-
2
-
-
1
1
-
4
-
-
1
-
-
5
-
-
2
1
1
-
-
1
1
-
-
2
-
-
-
-
1
1
2
-
-
-
-
-
-
2
-
-
1
1
-
-
-
1
-
5
-
-
2
1
1
-
-
1
1
-
-
-
-
1
1
-
1
1
286162
Dehal
Metabolism of monoterpenes: Sp ...
Tanacetum vulgare
Arch. Biochem. Biophys.
258
287-291
1987
-
-
-
-
-
1
-
-
2
-
1
1
-
1
-
-
1
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
1
1
-
-
-
1
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-