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show all sequences of 1.1.1.226

Purification and properties of 4-hydroxycyclohexanecarboxylate dehydrogenase from Corynebacterium cyclohexanicum

Obata, H.; Uebayasi, M.; Kaneda, T.; Eur. J. Biochem. 174, 451-458 (1988)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetamide
slight inhibition
Corynebacterium cyclohexanicum
iodoacetic acid
slight inhibition
Corynebacterium cyclohexanicum
N-bromosuccinimide
strong inhibition
Corynebacterium cyclohexanicum
p-hydroxymercuribenzoate
slight inhibition
Corynebacterium cyclohexanicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
NAD+
-
Corynebacterium cyclohexanicum
0.28
-
NADH
-
Corynebacterium cyclohexanicum
0.5
-
4-Oxocyclohexanecarboxylate
-
Corynebacterium cyclohexanicum
0.51
-
trans-4-hydroxycyclohexanecarboxylate
-
Corynebacterium cyclohexanicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27600
-
SDS-PAGE
Corynebacterium cyclohexanicum
53600
-
gel filtration
Corynebacterium cyclohexanicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
trans-4-hydroxycyclohexanecarboxylate + NAD+
Corynebacterium cyclohexanicum
-
4-oxocyclohexanecarboxylate + NADH
-
Corynebacterium cyclohexanicum
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corynebacterium cyclohexanicum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Corynebacterium cyclohexanicum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.48
-
crude extract, NADH oxidation
Corynebacterium cyclohexanicum
40
-
purified enzyme, NAD reduction
Corynebacterium cyclohexanicum
104.1
-
purified enzyme, NADH oxidation
Corynebacterium cyclohexanicum
Storage Stability
Storage Stability
Organism
-80░C
Corynebacterium cyclohexanicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trans-4-hydroxycyclohexanecarboxylate + NAD+
-
389502
Corynebacterium cyclohexanicum
4-oxocyclohexanecarboxylate + NADH
-
389502
Corynebacterium cyclohexanicum
r
trans-4-hydroxycyclohexanecarboxylate + NAD+
higher specific activities assayed for reverse reaction
389502
Corynebacterium cyclohexanicum
4-oxocyclohexanecarboxylate + NADH
-
389502
Corynebacterium cyclohexanicum
r
trans-4-hydroxycyclohexanecarboxylate + NAD+
highly specific for trans isomer
389502
Corynebacterium cyclohexanicum
4-oxocyclohexanecarboxylate + NADH
-
389502
Corynebacterium cyclohexanicum
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 27600, gel filtration, SDS-PAGE
Corynebacterium cyclohexanicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
7.2
NADH oxidation, depending on the buffer system
Corynebacterium cyclohexanicum
8
-
NAD reduction
Corynebacterium cyclohexanicum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
-
Corynebacterium cyclohexanicum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
very specific, no activity with NADP+
Corynebacterium cyclohexanicum
NADH
very specific, no activity with NADPH
Corynebacterium cyclohexanicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
very specific, no activity with NADP+
Corynebacterium cyclohexanicum
NADH
very specific, no activity with NADPH
Corynebacterium cyclohexanicum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetamide
slight inhibition
Corynebacterium cyclohexanicum
iodoacetic acid
slight inhibition
Corynebacterium cyclohexanicum
N-bromosuccinimide
strong inhibition
Corynebacterium cyclohexanicum
p-hydroxymercuribenzoate
slight inhibition
Corynebacterium cyclohexanicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
NAD+
-
Corynebacterium cyclohexanicum
0.28
-
NADH
-
Corynebacterium cyclohexanicum
0.5
-
4-Oxocyclohexanecarboxylate
-
Corynebacterium cyclohexanicum
0.51
-
trans-4-hydroxycyclohexanecarboxylate
-
Corynebacterium cyclohexanicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27600
-
SDS-PAGE
Corynebacterium cyclohexanicum
53600
-
gel filtration
Corynebacterium cyclohexanicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
trans-4-hydroxycyclohexanecarboxylate + NAD+
Corynebacterium cyclohexanicum
-
4-oxocyclohexanecarboxylate + NADH
-
Corynebacterium cyclohexanicum
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Corynebacterium cyclohexanicum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.48
-
crude extract, NADH oxidation
Corynebacterium cyclohexanicum
40
-
purified enzyme, NAD reduction
Corynebacterium cyclohexanicum
104.1
-
purified enzyme, NADH oxidation
Corynebacterium cyclohexanicum
Storage Stability (protein specific)
Storage Stability
Organism
-80░C
Corynebacterium cyclohexanicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trans-4-hydroxycyclohexanecarboxylate + NAD+
-
389502
Corynebacterium cyclohexanicum
4-oxocyclohexanecarboxylate + NADH
-
389502
Corynebacterium cyclohexanicum
r
trans-4-hydroxycyclohexanecarboxylate + NAD+
higher specific activities assayed for reverse reaction
389502
Corynebacterium cyclohexanicum
4-oxocyclohexanecarboxylate + NADH
-
389502
Corynebacterium cyclohexanicum
r
trans-4-hydroxycyclohexanecarboxylate + NAD+
highly specific for trans isomer
389502
Corynebacterium cyclohexanicum
4-oxocyclohexanecarboxylate + NADH
-
389502
Corynebacterium cyclohexanicum
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 27600, gel filtration, SDS-PAGE
Corynebacterium cyclohexanicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
7.2
NADH oxidation, depending on the buffer system
Corynebacterium cyclohexanicum
8
-
NAD reduction
Corynebacterium cyclohexanicum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
-
Corynebacterium cyclohexanicum
Other publictions for EC 1.1.1.226
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
389502
Obata
Purification and properties of ...
Corynebacterium cyclohexanicum
Eur. J. Biochem.
174
451-458
1988
-
-
-
-
-
-
4
4
-
-
2
1
-
2
-
-
1
-
-
-
3
1
3
1
-
-
-
-
2
1
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
4
-
-
2
1
-
-
-
1
-
-
3
1
3
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
389503
Taylor
-
Metabolism of cyclohexane caro ...
Alcaligenes sp., Alcaligenes sp. W1
J. Bacteriol.
5
401-411
1978
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-