BRENDA - Enzyme Database show
show all sequences of 1.1.1.225

Characterization of a unique aldo-keto reductase responsible for the reduction of chlordecone in the liver of the gerbil and man

Molowa, D.T.; Wrighton, S.A.; Blanke, R.V.; Guzelian, P.S.; J. Toxicol. Environ. Health 17, 375-384 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ethacrynic acid
-
Homo sapiens
Ethacrynic acid
-
Meriones unguiculatus
indomethacin
-
Homo sapiens
indomethacin
-
Meriones unguiculatus
additional information
not inhibited by phenobarbital
Homo sapiens
additional information
not inhibited by phenobarbital
Meriones unguiculatus
p-chloromercuribenzoate
strong inhibition
Meriones unguiculatus
p-chloromercuribenzoate
-
Homo sapiens
quercetin
weak inhibition
Meriones unguiculatus
quercetin
-
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0026
-
Chlordecone
-
Meriones unguiculatus
0.005
-
NADPH
-
Meriones unguiculatus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Homo sapiens
5737
-
cytoplasm
-
Meriones unguiculatus
5737
-
cytoplasm
-
Oryctolagus cuniculus
5737
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Meriones unguiculatus
-
-
-
no activity in Cavia porcellus
-
-
-
no activity in Mesocricetus auratus
-
-
-
no activity in Mus musculus
-
-
-
no activity in Rattus norvegicus
-
-
-
Oryctolagus cuniculus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Meriones unguiculatus
-
heart
-
Meriones unguiculatus
-
intestine
-
Meriones unguiculatus
-
kidney
-
Meriones unguiculatus
-
liver
-
Homo sapiens
-
liver
-
Meriones unguiculatus
-
liver
-
Oryctolagus cuniculus
-
lung
-
Meriones unguiculatus
-
additional information
found in all tissues, highest activity in liver, intestine and kidney
Meriones unguiculatus
-
spleen
-
Meriones unguiculatus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,1alpha,3,3beta,4,5,5,5alpha,5beta,6-decachlorooctahydro-1,3,4-metheno-2H-cyclobuta[cd]pentalen-2-one + NADPH
organochlorine pesticide, trivial names: chlordecone or kepone
286316
Homo sapiens
chlordecone alcohol + NADP+
-
286316
Homo sapiens
?
1,1alpha,3,3beta,4,5,5,5alpha,5beta,6-decachlorooctahydro-1,3,4-metheno-2H-cyclobuta[cd]pentalen-2-one + NADPH
organochlorine pesticide, trivial names: chlordecone or kepone
286316
Oryctolagus cuniculus
chlordecone alcohol + NADP+
-
286316
Oryctolagus cuniculus
?
1,1alpha,3,3beta,4,5,5,5alpha,5beta,6-decachlorooctahydro-1,3,4-metheno-2H-cyclobuta[cd]pentalen-2-one + NADPH
organochlorine pesticide, trivial names: chlordecone or kepone
286316
Meriones unguiculatus
chlordecone alcohol + NADP+
-
286316
Meriones unguiculatus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Meriones unguiculatus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Homo sapiens
NADPH
-
Meriones unguiculatus
NADPH
-
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Homo sapiens
NADPH
-
Meriones unguiculatus
NADPH
-
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ethacrynic acid
-
Homo sapiens
Ethacrynic acid
-
Meriones unguiculatus
indomethacin
-
Homo sapiens
indomethacin
-
Meriones unguiculatus
additional information
not inhibited by phenobarbital
Homo sapiens
additional information
not inhibited by phenobarbital
Meriones unguiculatus
p-chloromercuribenzoate
strong inhibition
Meriones unguiculatus
p-chloromercuribenzoate
-
Homo sapiens
quercetin
weak inhibition
Meriones unguiculatus
quercetin
-
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0026
-
Chlordecone
-
Meriones unguiculatus
0.005
-
NADPH
-
Meriones unguiculatus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Homo sapiens
5737
-
cytoplasm
-
Meriones unguiculatus
5737
-
cytoplasm
-
Oryctolagus cuniculus
5737
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Meriones unguiculatus
-
heart
-
Meriones unguiculatus
-
intestine
-
Meriones unguiculatus
-
kidney
-
Meriones unguiculatus
-
liver
-
Homo sapiens
-
liver
-
Meriones unguiculatus
-
liver
-
Oryctolagus cuniculus
-
lung
-
Meriones unguiculatus
-
additional information
found in all tissues, highest activity in liver, intestine and kidney
Meriones unguiculatus
-
spleen
-
Meriones unguiculatus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,1alpha,3,3beta,4,5,5,5alpha,5beta,6-decachlorooctahydro-1,3,4-metheno-2H-cyclobuta[cd]pentalen-2-one + NADPH
organochlorine pesticide, trivial names: chlordecone or kepone
286316
Homo sapiens
chlordecone alcohol + NADP+
-
286316
Homo sapiens
?
1,1alpha,3,3beta,4,5,5,5alpha,5beta,6-decachlorooctahydro-1,3,4-metheno-2H-cyclobuta[cd]pentalen-2-one + NADPH
organochlorine pesticide, trivial names: chlordecone or kepone
286316
Oryctolagus cuniculus
chlordecone alcohol + NADP+
-
286316
Oryctolagus cuniculus
?
1,1alpha,3,3beta,4,5,5,5alpha,5beta,6-decachlorooctahydro-1,3,4-metheno-2H-cyclobuta[cd]pentalen-2-one + NADPH
organochlorine pesticide, trivial names: chlordecone or kepone
286316
Meriones unguiculatus
chlordecone alcohol + NADP+
-
286316
Meriones unguiculatus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Meriones unguiculatus
Other publictions for EC 1.1.1.225
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286319
Khanna
Localization of multiple human ...
Homo sapiens
Genomics
25
588-590
1995
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286317
Winters
Isolation and characterization ...
Homo sapiens
Biochemistry
29
1080-1087
1990
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286316
Molowa
Characterization of a unique a ...
Homo sapiens, Meriones unguiculatus, no activity in Cavia porcellus, no activity in Mesocricetus auratus, no activity in Mus musculus, no activity in Rattus norvegicus, Oryctolagus cuniculus
J. Toxicol. Environ. Health
17
375-384
1986
-
-
-
-
-
-
10
2
3
-
-
-
-
7
-
-
-
-
-
10
-
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
10
-
2
3
-
-
-
-
-
-
-
-
10
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286318
Molowa
Purification and characterizat ...
Homo sapiens, Meriones unguiculatus, no activity in Mesocricetus auratus, no activity in Mus musculus, no activity in Rattus norvegicus, Oryctolagus cuniculus
J. Biol. Chem.
261
12624-12627
1986
-
-
-
-
-
-
14
2
3
-
-
-
-
8
-
-
1
-
-
4
1
1
3
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
4
-
-
-
-
14
-
2
3
-
-
-
-
-
-
1
-
4
1
1
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-