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show all sequences of 1.1.1.224

Gene silencing of mannose 6-phosphate reductase in the parasitic weed Orobanche aegyptiaca through the production of homologous dsRNA sequences in the host plant

Aly, R.; Cholakh, H.; Joel, D.M.; Leibman, D.; Steinitz, B.; Zelcer, A.; Naglis, A.; Yarden, O.; Gal-On, A.; Plant Biotechnol. J. 7, 487-498 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
transgenic tomato plants bearing a gene construct containing a specific 277-bp fragment from M6PR-mRNA, in an inverted-repeat configuration. Eight independent lines containing the M6PR-silencing inverted-repeat construct pBin-IR-M6PR construct are regenerated
Phelipanche aegyptiaca
Inhibitors
Inhibitors
Commentary
Organism
Structure
siRNA
transformed tomato plants containing siRNA from the M6PR of Orobanche aegyptiaca show normal growth and development, accumulation of M6PR siRNA in transgenic tomatoes correlates with decreased levels of M6PR mRNA. Amount of endogenous M6PR mRNA in the tubercles and underground shoots of Orobanche aegyptiaca grown on transgenic host plants is reduced by 60%-80%. Concomitant with M6PR mRNA suppression, there is a significant decrease in mannitol level and a significant increase in the percentage of dead Orobanche aegyptiaca tubercles on the transgenic host plants. Detection of mir390, which is involved with cytoplasmic dsRNA processing, indicates to the existence of gene-silencing mechanisms in Orobanche spp. Gene silencing mechanisms are probably involved with the production of decreased levels of M6PR mRNA in the parasites grown on the transformed tomato lines
Phelipanche aegyptiaca
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phelipanche aegyptiaca
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
shoot
underground
Phelipanche aegyptiaca
-
tubercle
-
Phelipanche aegyptiaca
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-mannose 6-phosphate + NADPH + H+
-
700701
Phelipanche aegyptiaca
D-mannitol 1-phosphate + NADP+
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
transgenic tomato plants bearing a gene construct containing a specific 277-bp fragment from M6PR-mRNA, in an inverted-repeat configuration. Eight independent lines containing the M6PR-silencing inverted-repeat construct pBin-IR-M6PR construct are regenerated
Phelipanche aegyptiaca
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
siRNA
transformed tomato plants containing siRNA from the M6PR of Orobanche aegyptiaca show normal growth and development, accumulation of M6PR siRNA in transgenic tomatoes correlates with decreased levels of M6PR mRNA. Amount of endogenous M6PR mRNA in the tubercles and underground shoots of Orobanche aegyptiaca grown on transgenic host plants is reduced by 60%-80%. Concomitant with M6PR mRNA suppression, there is a significant decrease in mannitol level and a significant increase in the percentage of dead Orobanche aegyptiaca tubercles on the transgenic host plants. Detection of mir390, which is involved with cytoplasmic dsRNA processing, indicates to the existence of gene-silencing mechanisms in Orobanche spp. Gene silencing mechanisms are probably involved with the production of decreased levels of M6PR mRNA in the parasites grown on the transformed tomato lines
Phelipanche aegyptiaca
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
shoot
underground
Phelipanche aegyptiaca
-
tubercle
-
Phelipanche aegyptiaca
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-mannose 6-phosphate + NADPH + H+
-
700701
Phelipanche aegyptiaca
D-mannitol 1-phosphate + NADP+
-
-
-
?
Other publictions for EC 1.1.1.224
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725628
Chan
Global gene expression analysi ...
Apium graveolens
J. Exp. Bot.
62
4787-4803
2011
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1
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713260
Song
A novel mannose-based selectio ...
Apium graveolens
Plant Cell Rep.
29
163-172
2010
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1
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6
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700701
Aly
Gene silencing of mannose 6-ph ...
Phelipanche aegyptiaca
Plant Biotechnol. J.
7
487-498
2009
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1
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1
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1
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686908
Sickler
Response of mannitol-producing ...
Apium graveolens
Funct. Plant Biol.
34
382-391
2007
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1
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1
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1
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656972
Zhifang
-
Expression of a celery mannose ...
Apium graveolens, no activity in Arabidopsis thaliana
Plant Cell Environ.
26
275-283
2003
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1
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1
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1
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348008
Delavault
Isolation of mannose 6-phospha ...
Phelipanche ramosa
Physiol. Plant.
115
48-55
2002
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1
1
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2
2
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6
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1
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4
3
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4
1
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2
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1
1
2
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2
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4
3
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1
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348006
Robert
-
Purification and characterizat ...
Phelipanche ramosa, Striga hermonthica
Aust. J. Plant Physiol.
26
233-237
1999
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4
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2
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2
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2
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2
2
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2
2
2
1
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2
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2
4
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4
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4
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2
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2
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2
2
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2
2
2
1
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2
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2
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348007
Robert
-
Inhibition studies on mannose ...
Phelipanche ramosa
Carbohydr. Lett.
3
231-238
1999
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1
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1
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1
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2
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1
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1
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1
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348005
Everard
Molecular cloning of mannose-6 ...
Apium graveolens
Plant Physiol.
113
1427-1435
1997
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1
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4
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1
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6
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1
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4
2
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1
1
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1
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1
1
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4
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1
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1
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4
2
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1
1
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348003
Stoop
Mannitol metabolism in celery ...
Apium graveolens
Plant Physiol.
106
503-511
1994
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3
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1
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1
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348004
Everard
Mannose-6-phosphate reductase, ...
Apium graveolens
Plant Physiol.
102
345-356
1993
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3
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4
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3
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348002
Loescher
Mannitol synthesis in higher p ...
Apium graveolens, Ligustrum vulgare
Plant Physiol.
98
1396-1402
1992
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-
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2
1
2
4
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3
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6
1
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1
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2
2
2
2
1
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2
1
1
4
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4
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2
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1
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2
4
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3
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1
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1
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2
2
2
2
1
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2
1
1
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94715
Rumpho
A pathway for photosynthetic c ...
Apium graveolens
Plant Physiol.
73
869-873
1983
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2
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1
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1
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1
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1
1
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2
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1
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2
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