BRENDA - Enzyme Database show
show all sequences of 1.1.1.224

Isolation of mannose 6-phosphate reductase cDNA, changes in enzyme activity and mannitol content in broomrape (Orobanche ramosa) parasitic on tomato roots

Delavault, P.; Simier, P.; Thoiron, S.; Veronesi, C.; Fer, A.; Thalouarn, P.; Physiol. Plant. 115, 48-55 (2002)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
enzyme is a target for herbizide treatment
Phelipanche ramosa
Cloned(Commentary)
Commentary
Organism
; single gene, constitutive, DNA sequence determination and analysis, expression in Escherichia coli
Phelipanche ramosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
theoretical molecular weight, calculated from cDNA sequence
Phelipanche ramosa
37000
-
1 * 37000, SDS-PAGE of recombinant enzyme
Phelipanche ramosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-mannitol 1-phosphate + NADP+
Phelipanche ramosa
key enzyme in mannitol production in the parasite
D-mannose 6-phosphate + NADPH + H+
-
-
?
D-mannose 6-phosphate + NADPH
Phelipanche ramosa
key enzyme in mannitol production in higher plants
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phelipanche ramosa
-
broomrape; parasitic broomrape on tomato roots
-
Purification (Commentary)
Commentary
Organism
purification of recombinant enzyme
Phelipanche ramosa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
constitutive gene expression during different developmental stages, e.g. germination and procaulome growth, of the parasite attached to tomato roots, high activity during tubercle enlargement and crown root formation; overview over different developmental stages
Phelipanche ramosa
-
root
-
Phelipanche ramosa
-
seed
-
Phelipanche ramosa
-
shoot
-
Phelipanche ramosa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
measurement of activity during plant development
Phelipanche ramosa
0.00822
-
crude extract from expression in Escherichia coli
Phelipanche ramosa
0.12
-
in tubercle
Phelipanche ramosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-mannitol 1-phosphate + NADP+
-
348008
Phelipanche ramosa
D-mannose 6-phosphate + NADPH + H+
-
-
-
?
D-mannitol 1-phosphate + NADP+
key enzyme in mannitol production in the parasite
348008
Phelipanche ramosa
D-mannose 6-phosphate + NADPH + H+
-
-
-
?
D-mannose 6-phosphate + NADPH
key enzyme in mannitol production in higher plants
348008
Phelipanche ramosa
?
-
-
-
-
D-mannose 6-phosphate + NADPH
-
348008
Phelipanche ramosa
D-mannitol 1-phosphate + NADP+
-
348008
Phelipanche ramosa
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 37000, SDS-PAGE of recombinant enzyme
Phelipanche ramosa
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Phelipanche ramosa
NADPH
-
Phelipanche ramosa
Application (protein specific)
Application
Commentary
Organism
agriculture
enzyme is a target for herbizide treatment
Phelipanche ramosa
Cloned(Commentary) (protein specific)
Commentary
Organism
; single gene, constitutive, DNA sequence determination and analysis, expression in Escherichia coli
Phelipanche ramosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Phelipanche ramosa
NADPH
-
Phelipanche ramosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
theoretical molecular weight, calculated from cDNA sequence
Phelipanche ramosa
37000
-
1 * 37000, SDS-PAGE of recombinant enzyme
Phelipanche ramosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-mannitol 1-phosphate + NADP+
Phelipanche ramosa
key enzyme in mannitol production in the parasite
D-mannose 6-phosphate + NADPH + H+
-
-
?
D-mannose 6-phosphate + NADPH
Phelipanche ramosa
key enzyme in mannitol production in higher plants
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
purification of recombinant enzyme
Phelipanche ramosa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
constitutive gene expression during different developmental stages, e.g. germination and procaulome growth, of the parasite attached to tomato roots, high activity during tubercle enlargement and crown root formation; overview over different developmental stages
Phelipanche ramosa
-
root
-
Phelipanche ramosa
-
seed
-
Phelipanche ramosa
-
shoot
-
Phelipanche ramosa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
measurement of activity during plant development
Phelipanche ramosa
0.00822
-
crude extract from expression in Escherichia coli
Phelipanche ramosa
0.12
-
in tubercle
Phelipanche ramosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-mannitol 1-phosphate + NADP+
-
348008
Phelipanche ramosa
D-mannose 6-phosphate + NADPH + H+
-
-
-
?
D-mannitol 1-phosphate + NADP+
key enzyme in mannitol production in the parasite
348008
Phelipanche ramosa
D-mannose 6-phosphate + NADPH + H+
-
-
-
?
D-mannose 6-phosphate + NADPH
key enzyme in mannitol production in higher plants
348008
Phelipanche ramosa
?
-
-
-
-
D-mannose 6-phosphate + NADPH
-
348008
Phelipanche ramosa
D-mannitol 1-phosphate + NADP+
-
348008
Phelipanche ramosa
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 37000, SDS-PAGE of recombinant enzyme
Phelipanche ramosa
Other publictions for EC 1.1.1.224
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725628
Chan
Global gene expression analysi ...
Apium graveolens
J. Exp. Bot.
62
4787-4803
2011
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1
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4
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1
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-
713260
Song
A novel mannose-based selectio ...
Apium graveolens
Plant Cell Rep.
29
163-172
2010
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1
-
-
-
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6
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
700701
Aly
Gene silencing of mannose 6-ph ...
Phelipanche aegyptiaca
Plant Biotechnol. J.
7
487-498
2009
-
-
1
-
-
-
1
-
-
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5
-
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2
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1
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1
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1
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2
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686908
Sickler
Response of mannitol-producing ...
Apium graveolens
Funct. Plant Biol.
34
382-391
2007
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
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-
-
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1
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1
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-
-
-
-
-
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-
-
656972
Zhifang
-
Expression of a celery mannose ...
Apium graveolens, no activity in Arabidopsis thaliana
Plant Cell Environ.
26
275-283
2003
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
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-
-
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-
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1
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-
1
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-
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1
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348008
Delavault
Isolation of mannose 6-phospha ...
Phelipanche ramosa
Physiol. Plant.
115
48-55
2002
-
1
1
-
-
-
-
-
-
-
2
2
-
6
-
-
1
-
-
4
3
-
4
1
-
-
-
-
-
-
-
2
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
1
-
4
3
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348006
Robert
-
Purification and characterizat ...
Phelipanche ramosa, Striga hermonthica
Aust. J. Plant Physiol.
26
233-237
1999
-
-
-
-
-
-
-
4
-
-
2
-
-
2
-
-
2
-
-
2
2
-
2
2
2
1
-
-
2
-
2
4
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
2
-
-
-
-
2
-
2
2
-
2
2
2
1
-
-
2
-
2
-
-
-
-
-
-
-
348007
Robert
-
Inhibition studies on mannose ...
Phelipanche ramosa
Carbohydr. Lett.
3
231-238
1999
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
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1
-
-
2
-
-
-
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1
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-
1
-
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-
1
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-
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-
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-
-
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-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348005
Everard
Molecular cloning of mannose-6 ...
Apium graveolens
Plant Physiol.
113
1427-1435
1997
-
-
1
-
-
-
-
4
-
-
1
-
-
6
-
-
1
-
-
4
2
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
1
-
-
-
-
1
-
4
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348003
Stoop
Mannitol metabolism in celery ...
Apium graveolens
Plant Physiol.
106
503-511
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
1
-
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1
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1
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2
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348004
Everard
Mannose-6-phosphate reductase, ...
Apium graveolens
Plant Physiol.
102
345-356
1993
-
-
-
-
-
-
-
-
3
-
-
-
-
4
-
-
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-
3
-
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3
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3
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-
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-
-
-
-
-
348002
Loescher
Mannitol synthesis in higher p ...
Apium graveolens, Ligustrum vulgare
Plant Physiol.
98
1396-1402
1992
-
-
-
-
-
2
1
2
4
-
3
-
-
6
1
-
1
-
-
2
2
2
2
1
-
-
-
-
2
1
1
4
-
-
-
-
-
-
4
-
-
2
-
1
-
2
4
-
3
-
-
1
-
1
-
2
2
2
2
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
94715
Rumpho
A pathway for photosynthetic c ...
Apium graveolens
Plant Physiol.
73
869-873
1983
-
-
-
-
-
-
-
-
2
-
-
1
-
1
-
-
1
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-
1
1
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2
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1
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1
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1
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2
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1
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1
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1
1
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2
-
1
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