KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0102 | - |
NADPH | enzyme purified from leaves | Striga hermonthica | |
0.0698 | - |
NADPH | enzyme purified from flowering spikes | Phelipanche ramosa | |
5.9 | - |
D-mannose 6-phosphate | enzyme purified from leaves | Striga hermonthica | |
8.6 | - |
D-mannose 6-phosphate | enzyme purified from flowering spikes | Phelipanche ramosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration with Sephacryl S-200 | Phelipanche ramosa |
33000 | - |
gel filtration with Sephacryl S-200 | Striga hermonthica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phelipanche ramosa | - |
broomrape | - |
Striga hermonthica | - |
witchweed | - |
Purification (Comment) | Organism |
---|---|
- |
Striga hermonthica |
- |
Phelipanche ramosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Striga hermonthica | - |
spike | flowering | Phelipanche ramosa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.008 | - |
crude extract from mature leaves | Striga hermonthica |
0.014 | - |
crude extract from flowering spikes | Phelipanche ramosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannose 6-phosphate + NADPH | - |
Striga hermonthica | D-mannitol 1-phosphate + NADP+ | - |
? | |
D-mannose 6-phosphate + NADPH | - |
Phelipanche ramosa | D-mannitol 1-phosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1* 39000, SDS-PAGE | Striga hermonthica |
monomer | 1* 39000, SDS-PAGE | Phelipanche ramosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Striga hermonthica |
45 | - |
- |
Phelipanche ramosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | 75% of maximal activity | Striga hermonthica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Striga hermonthica |
7 | - |
- |
Phelipanche ramosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
rapid decrease in the range of basic pH values | Striga hermonthica |
additional information | - |
rapid decrease in the range of basic pH values | Phelipanche ramosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | 5% activity compared to NADPH | Phelipanche ramosa | |
NADH | 10% of activity compared to NADPH | Striga hermonthica | |
NADPH | - |
Striga hermonthica | |
NADPH | - |
Phelipanche ramosa |