BRENDA - Enzyme Database show
show all sequences of 1.1.1.224

Mannitol synthesis in higher plants. Evidence for the role and characterization of a NADPH-dependent mannose 6-phosphate reductase

Loescher, W.H.; Tyson, R.H.; Everard, J.D.; Redgewell, J.; Bieleski, R.L.; Plant Physiol. 98, 1396-1402 (1992)

Data extracted from this reference:

General Stability
General Stability
Organism
degassed buffers stabilize
Apium graveolens
DTT stabilizes
Apium graveolens
Inhibitors
Inhibitors
Commentary
Organism
Structure
NaCl
47% reduction by 100 mM
Apium graveolens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
15.8
-
D-mannose 6-phosphate
-
Apium graveolens
45
-
D-mannitol 1-phosphate
-
Apium graveolens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Apium graveolens
5737
-
cytoplasm
-
Ligustrum vulgare
5737
-
protoplast
-
Ligustrum vulgare
-
-
protoplast
-
Apium graveolens
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, SDS-PAGE
Apium graveolens
53000
-
gel filtration with Superose 12
Apium graveolens
58000
-
gel filtration with Sephacryl S-200
Apium graveolens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Apium graveolens
-
celery; cv. Giant Pascal
-
Ligustrum vulgare
-
privet
-
Oxidation Stability
Oxidation Stability
Organism
DTT stabilizes
Apium graveolens
Purification (Commentary)
Commentary
Organism
-
Apium graveolens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Apium graveolens
-
shoot
-
Ligustrum vulgare
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.053
-
-
Apium graveolens
3.76
-
purified from leaves
Apium graveolens
Storage Stability
Storage Stability
Organism
-21°C, 50% activity retained after 1 month
Apium graveolens
4°C, lyophilized active fractions stable for several months
Apium graveolens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-mannose 6-phosphate + NADPH
-
348002
Apium graveolens
D-mannitol 1-phosphate + NADP+
-
348002
Apium graveolens
r
D-mannose 6-phosphate + NADPH
no activity with D-fructose 6-phosphate, D-glucose 6-phosphate, D-fructose 1-phosphate, D-mannose 1-phosphate, D-mannose
348002
Ligustrum vulgare
D-mannitol 1-phosphate + NADP+
-
348002
Ligustrum vulgare
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 35000, SDS-PAGE
Apium graveolens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
D-mannose 6-phosphate
Apium graveolens
8.5
-
D-mannitol 1-phosphate
Apium graveolens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
inactivated at pH below 6
Apium graveolens
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
-
Apium graveolens
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
NAD+ partially (8% activity) substitutes for NADP+ in oxidizing mannitol 1-phosphate
Apium graveolens
NADP+
NAD+ partially (8% activity) substitutes for NADP+ in oxidizing mannitol 1-phosphate
Ligustrum vulgare
NADPH
NADH cannot replace NADPH in reducing mannose 6-phosphate
Apium graveolens
NADPH
NADH cannot replace NADPH in reducing mannose 6-phosphate
Ligustrum vulgare
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
NAD+ partially (8% activity) substitutes for NADP+ in oxidizing mannitol 1-phosphate
Apium graveolens
NADP+
NAD+ partially (8% activity) substitutes for NADP+ in oxidizing mannitol 1-phosphate
Ligustrum vulgare
NADPH
NADH cannot replace NADPH in reducing mannose 6-phosphate
Apium graveolens
NADPH
NADH cannot replace NADPH in reducing mannose 6-phosphate
Ligustrum vulgare
General Stability (protein specific)
General Stability
Organism
degassed buffers stabilize
Apium graveolens
DTT stabilizes
Apium graveolens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NaCl
47% reduction by 100 mM
Apium graveolens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
15.8
-
D-mannose 6-phosphate
-
Apium graveolens
45
-
D-mannitol 1-phosphate
-
Apium graveolens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Apium graveolens
5737
-
cytoplasm
-
Ligustrum vulgare
5737
-
protoplast
-
Ligustrum vulgare
-
-
protoplast
-
Apium graveolens
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, SDS-PAGE
Apium graveolens
53000
-
gel filtration with Superose 12
Apium graveolens
58000
-
gel filtration with Sephacryl S-200
Apium graveolens
Oxidation Stability (protein specific)
Oxidation Stability
Organism
DTT stabilizes
Apium graveolens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Apium graveolens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Apium graveolens
-
shoot
-
Ligustrum vulgare
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.053
-
-
Apium graveolens
3.76
-
purified from leaves
Apium graveolens
Storage Stability (protein specific)
Storage Stability
Organism
-21°C, 50% activity retained after 1 month
Apium graveolens
4°C, lyophilized active fractions stable for several months
Apium graveolens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-mannose 6-phosphate + NADPH
-
348002
Apium graveolens
D-mannitol 1-phosphate + NADP+
-
348002
Apium graveolens
r
D-mannose 6-phosphate + NADPH
no activity with D-fructose 6-phosphate, D-glucose 6-phosphate, D-fructose 1-phosphate, D-mannose 1-phosphate, D-mannose
348002
Ligustrum vulgare
D-mannitol 1-phosphate + NADP+
-
348002
Ligustrum vulgare
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 35000, SDS-PAGE
Apium graveolens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
D-mannose 6-phosphate
Apium graveolens
8.5
-
D-mannitol 1-phosphate
Apium graveolens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
inactivated at pH below 6
Apium graveolens
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
-
Apium graveolens
Other publictions for EC 1.1.1.224
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725628
Chan
Global gene expression analysi ...
Apium graveolens
J. Exp. Bot.
62
4787-4803
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713260
Song
A novel mannose-based selectio ...
Apium graveolens
Plant Cell Rep.
29
163-172
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700701
Aly
Gene silencing of mannose 6-ph ...
Phelipanche aegyptiaca
Plant Biotechnol. J.
7
487-498
2009
-
-
1
-
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686908
Sickler
Response of mannitol-producing ...
Apium graveolens
Funct. Plant Biol.
34
382-391
2007
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656972
Zhifang
-
Expression of a celery mannose ...
Apium graveolens, no activity in Arabidopsis thaliana
Plant Cell Environ.
26
275-283
2003
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348008
Delavault
Isolation of mannose 6-phospha ...
Phelipanche ramosa
Physiol. Plant.
115
48-55
2002
-
1
1
-
-
-
-
-
-
-
2
2
-
6
-
-
1
-
-
4
3
-
4
1
-
-
-
-
-
-
-
2
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
1
-
4
3
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348006
Robert
-
Purification and characterizat ...
Phelipanche ramosa, Striga hermonthica
Aust. J. Plant Physiol.
26
233-237
1999
-
-
-
-
-
-
-
4
-
-
2
-
-
2
-
-
2
-
-
2
2
-
2
2
2
1
-
-
2
-
2
4
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
2
-
-
-
-
2
-
2
2
-
2
2
2
1
-
-
2
-
2
-
-
-
-
-
-
-
348007
Robert
-
Inhibition studies on mannose ...
Phelipanche ramosa
Carbohydr. Lett.
3
231-238
1999
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348005
Everard
Molecular cloning of mannose-6 ...
Apium graveolens
Plant Physiol.
113
1427-1435
1997
-
-
1
-
-
-
-
4
-
-
1
-
-
6
-
-
1
-
-
4
2
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
1
-
-
-
-
1
-
4
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348003
Stoop
Mannitol metabolism in celery ...
Apium graveolens
Plant Physiol.
106
503-511
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348004
Everard
Mannose-6-phosphate reductase, ...
Apium graveolens
Plant Physiol.
102
345-356
1993
-
-
-
-
-
-
-
-
3
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348002
Loescher
Mannitol synthesis in higher p ...
Apium graveolens, Ligustrum vulgare
Plant Physiol.
98
1396-1402
1992
-
-
-
-
-
2
1
2
4
-
3
-
-
6
1
-
1
-
-
2
2
2
2
1
-
-
-
-
2
1
1
4
-
-
-
-
-
-
4
-
-
2
-
1
-
2
4
-
3
-
-
1
-
1
-
2
2
2
2
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
94715
Rumpho
A pathway for photosynthetic c ...
Apium graveolens
Plant Physiol.
73
869-873
1983
-
-
-
-
-
-
-
-
2
-
-
1
-
1
-
-
1
-
-
1
1
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-