BRENDA - Enzyme Database show
show all sequences of 1.1.1.223

Metabolism of monoterpenes: oxidation of isopiperitenol to isopiperitenone, and subsequent isomerization to piperitenone by soluble enzyme preparations from peppermint (Mentha piperita) leaves

Kjonaas, R.B.; Venkatachalam, K.V.; Croteau, R.; Arch. Biochem. Biophys. 238, 49-60 (1985)

Data extracted from this reference:

General Stability
General Stability
Organism
stabilized with bovine serum albumin
Mentha x piperita
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodacetamide
slight inhibition
Mentha x piperita
N-ethylmaleimide
slight inhibition
Mentha x piperita
p-hydroxymercuribenzoate
complete inhibition at 0.025 nM
Mentha x piperita
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
NAD+
-
Mentha x piperita
0.02
-
(-)-trans-isoperitenol
-
Mentha x piperita
0.02
-
(+)-trans-isoperitenol
-
Mentha x piperita
0.08
-
(-)-cis-isoperitenol
-
Mentha x piperita
0.08
-
(+)-cis-isoperitenol
-
Mentha x piperita
2.5
-
NADP+
-
Mentha x piperita
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Mentha x piperita
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Hg2+
complete inhibition at 0.1 mM
Mentha x piperita
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66000
-
gel filtration
Mentha x piperita
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-trans-isopiperitenol + NAD+
Mentha x piperita
central enzyme in menthol biosynthesis pathway
(-)-isopiperitenone + NADH
-
Mentha x piperita
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mentha x piperita
-
peppermint
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Mentha x piperita
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-isomenthol + NAD+
activity less than 2% of that of (-)-trans-isopiperitenol
2922
Mentha x piperita
(+)-isomenthone + NADH
-
-
-
?
(+)-trans-isopiperitenol + NAD+
95% activity of that of (-)-trans-isopiperitenol
2922
Mentha x piperita
(+)-isopiperitenone + NADH
-
2922
Mentha x piperita
?
(+)-trans-pulegol + NAD+
19% activity of that of (-)-trans-isopiperitenol
2922
Mentha x piperita
(R)-pulegone + NADH
-
-
-
?
(+,-)-cis-isopiperitenol + NAD+
-
2922
Mentha x piperita
(+,-)-isopiperitenone
-
-
-
?
(-)-trans-isopiperitenol + NAD+
preferred substrate, not readily reversible in pH range 6.5-10.5
2922
Mentha x piperita
(-)-isopiperitenone + NADH
-
2922
Mentha x piperita
r
(-)-trans-isopiperitenol + NAD+
central enzyme in menthol biosynthesis pathway
2922
Mentha x piperita
(-)-isopiperitenone + NADH
-
2922
Mentha x piperita
r
(-)-trans-isopiperitenol + NADP+
-
2922
Mentha x piperita
(-)-isopiperitenone + NADPH + H+
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
10.5
-
Mentha x piperita
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
11
half maximum activity at both pH
Mentha x piperita
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
FAD completely ineffective
Mentha x piperita
NAD+
-
Mentha x piperita
NADP+
less than 10% of the activity with NAD
Mentha x piperita
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
FAD completely ineffective
Mentha x piperita
NAD+
-
Mentha x piperita
NADP+
less than 10% of the activity with NAD
Mentha x piperita
General Stability (protein specific)
General Stability
Organism
stabilized with bovine serum albumin
Mentha x piperita
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodacetamide
slight inhibition
Mentha x piperita
N-ethylmaleimide
slight inhibition
Mentha x piperita
p-hydroxymercuribenzoate
complete inhibition at 0.025 nM
Mentha x piperita
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
NAD+
-
Mentha x piperita
0.02
-
(-)-trans-isoperitenol
-
Mentha x piperita
0.02
-
(+)-trans-isoperitenol
-
Mentha x piperita
0.08
-
(-)-cis-isoperitenol
-
Mentha x piperita
0.08
-
(+)-cis-isoperitenol
-
Mentha x piperita
2.5
-
NADP+
-
Mentha x piperita
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Mentha x piperita
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Hg2+
complete inhibition at 0.1 mM
Mentha x piperita
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66000
-
gel filtration
Mentha x piperita
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-trans-isopiperitenol + NAD+
Mentha x piperita
central enzyme in menthol biosynthesis pathway
(-)-isopiperitenone + NADH
-
Mentha x piperita
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Mentha x piperita
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-isomenthol + NAD+
activity less than 2% of that of (-)-trans-isopiperitenol
2922
Mentha x piperita
(+)-isomenthone + NADH
-
-
-
?
(+)-trans-isopiperitenol + NAD+
95% activity of that of (-)-trans-isopiperitenol
2922
Mentha x piperita
(+)-isopiperitenone + NADH
-
2922
Mentha x piperita
?
(+)-trans-pulegol + NAD+
19% activity of that of (-)-trans-isopiperitenol
2922
Mentha x piperita
(R)-pulegone + NADH
-
-
-
?
(+,-)-cis-isopiperitenol + NAD+
-
2922
Mentha x piperita
(+,-)-isopiperitenone
-
-
-
?
(-)-trans-isopiperitenol + NAD+
preferred substrate, not readily reversible in pH range 6.5-10.5
2922
Mentha x piperita
(-)-isopiperitenone + NADH
-
2922
Mentha x piperita
r
(-)-trans-isopiperitenol + NAD+
central enzyme in menthol biosynthesis pathway
2922
Mentha x piperita
(-)-isopiperitenone + NADH
-
2922
Mentha x piperita
r
(-)-trans-isopiperitenol + NADP+
-
2922
Mentha x piperita
(-)-isopiperitenone + NADPH + H+
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
10.5
-
Mentha x piperita
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
11
half maximum activity at both pH
Mentha x piperita
Other publictions for EC 1.1.1.223
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
670589
Ringer
Monoterpene metabolism. Clonin ...
Mentha x piperita
Plant Physiol.
137
863-872
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286315
McConkey
Developmental regulation of mo ...
Mentha x piperita
Plant Physiol.
122
215-223
2000
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2922
Kjonaas
Metabolism of monoterpenes: ox ...
Mentha x piperita
Arch. Biochem. Biophys.
238
49-60
1985
-
-
-
-
-
1
3
6
1
1
1
1
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
1
-
3
-
6
1
1
1
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-