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show all sequences of 1.1.1.222

Purification and properties of D-aromatic lactate dehydrogenase, an enzyme involved in the catabolism of the aromatic amino acids of Candida maltosa

Bode, R.; Lippoldt, A.; Birnbaum, D.; Biochem. Physiol. Pflanz. 181, 189-198 (1986)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
unstable in buffers without glycerol, stabilized by adding 30-50% glycerol to the buffer
Candida maltosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Candida maltosa
EDTA
-
Candida maltosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0077
-
NADH
-
Candida maltosa
0.0086
-
NADPH
-
Candida maltosa
0.021
-
NADP+
-
Candida maltosa
0.044
-
p-hydroxyphenylpyruvate
-
Candida maltosa
0.067
-
Indolepyruvate
-
Candida maltosa
0.076
-
p-hydroxyphenyllactate
-
Candida maltosa
0.083
-
D-phenyllactate
-
Candida maltosa
0.093
-
NAD+
-
Candida maltosa
0.094
-
indolelactate
-
Candida maltosa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
inhibitory
Candida maltosa
Mn2+
required
Candida maltosa
Zn2+
inhibitory
Candida maltosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
4 * 68000, SDS-PAGE, gel filtration chromatography
Candida maltosa
250000
-
gel filtration
Candida maltosa
280000
-
glycerol density gradient centrifugation
Candida maltosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-(4-hydroxyphenyl)pyruvate + NAD(P)H
Candida maltosa
involved in aromatic amino acid catabolism
(R)-3-(4-hydroxyphenyl)lactate + NAD(P)+
-
Candida maltosa
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida maltosa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Candida maltosa
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.5
-
crude extract
Candida maltosa
1170
-
purified enzyme
Candida maltosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-ketoisopentanoate + NAD(P)H
poor substrate
286312
Candida maltosa
2-hydroxyisopentanoate + NAD(P)+
-
-
-
r
2-oxobutyrate + NAD(P)H + H+
poor substrate
286312
Candida maltosa
2-hydroxybutyrate + NAD(P)+
-
-
-
r
3-(4-hydroxyphenyl)pyruvate + NAD(P)H
the initial rate of oxidation is 100 fold lower than that of reduction
286312
Candida maltosa
(R)-3-(4-hydroxyphenyl)lactate + NAD(P)+
-
286312
Candida maltosa
r
3-(4-hydroxyphenyl)pyruvate + NAD(P)H
involved in aromatic amino acid catabolism
286312
Candida maltosa
(R)-3-(4-hydroxyphenyl)lactate + NAD(P)+
-
286312
Candida maltosa
-
indolepyruvate + NAD(P)H
reduction at 30% the rate of p-hydroxyphenylpyruvate reduction
286312
Candida maltosa
indolelactate + NAD(P)+
-
286312
Candida maltosa
r
additional information
best substrate for oxidation is p-hydroxyphenyllactate, D-phenyllactate is oxidized at 75% and indolelactate at 52% the rate of p-hydroxyphenyllactate
286312
Candida maltosa
?
-
-
-
-
phenylpyruvate + NAD(P)H
reduction at 27% the rate of p-hydroxyphenylpyruvate reduction
286312
Candida maltosa
D-phenyllactate + NAD(P)+
-
286312
Candida maltosa
r
pyruvate + NAD(P)H + H+
poor substrate
286312
Candida maltosa
lactate + NAD(P)+
-
-
-
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 68000, SDS-PAGE, gel filtration chromatography
Candida maltosa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Candida maltosa
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
60
-
Candida maltosa
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
significant loss of activity above 40°C
Candida maltosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction
Candida maltosa
9.5
-
oxidation
Candida maltosa
General Stability (protein specific)
General Stability
Organism
unstable in buffers without glycerol, stabilized by adding 30-50% glycerol to the buffer
Candida maltosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Candida maltosa
EDTA
-
Candida maltosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0077
-
NADH
-
Candida maltosa
0.0086
-
NADPH
-
Candida maltosa
0.021
-
NADP+
-
Candida maltosa
0.044
-
p-hydroxyphenylpyruvate
-
Candida maltosa
0.067
-
Indolepyruvate
-
Candida maltosa
0.076
-
p-hydroxyphenyllactate
-
Candida maltosa
0.083
-
D-phenyllactate
-
Candida maltosa
0.093
-
NAD+
-
Candida maltosa
0.094
-
indolelactate
-
Candida maltosa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
inhibitory
Candida maltosa
Mn2+
required
Candida maltosa
Zn2+
inhibitory
Candida maltosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
4 * 68000, SDS-PAGE, gel filtration chromatography
Candida maltosa
250000
-
gel filtration
Candida maltosa
280000
-
glycerol density gradient centrifugation
Candida maltosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-(4-hydroxyphenyl)pyruvate + NAD(P)H
Candida maltosa
involved in aromatic amino acid catabolism
(R)-3-(4-hydroxyphenyl)lactate + NAD(P)+
-
Candida maltosa
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Candida maltosa
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.5
-
crude extract
Candida maltosa
1170
-
purified enzyme
Candida maltosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-ketoisopentanoate + NAD(P)H
poor substrate
286312
Candida maltosa
2-hydroxyisopentanoate + NAD(P)+
-
-
-
r
2-oxobutyrate + NAD(P)H + H+
poor substrate
286312
Candida maltosa
2-hydroxybutyrate + NAD(P)+
-
-
-
r
3-(4-hydroxyphenyl)pyruvate + NAD(P)H
the initial rate of oxidation is 100 fold lower than that of reduction
286312
Candida maltosa
(R)-3-(4-hydroxyphenyl)lactate + NAD(P)+
-
286312
Candida maltosa
r
3-(4-hydroxyphenyl)pyruvate + NAD(P)H
involved in aromatic amino acid catabolism
286312
Candida maltosa
(R)-3-(4-hydroxyphenyl)lactate + NAD(P)+
-
286312
Candida maltosa
-
indolepyruvate + NAD(P)H
reduction at 30% the rate of p-hydroxyphenylpyruvate reduction
286312
Candida maltosa
indolelactate + NAD(P)+
-
286312
Candida maltosa
r
additional information
best substrate for oxidation is p-hydroxyphenyllactate, D-phenyllactate is oxidized at 75% and indolelactate at 52% the rate of p-hydroxyphenyllactate
286312
Candida maltosa
?
-
-
-
-
phenylpyruvate + NAD(P)H
reduction at 27% the rate of p-hydroxyphenylpyruvate reduction
286312
Candida maltosa
D-phenyllactate + NAD(P)+
-
286312
Candida maltosa
r
pyruvate + NAD(P)H + H+
poor substrate
286312
Candida maltosa
lactate + NAD(P)+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 68000, SDS-PAGE, gel filtration chromatography
Candida maltosa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Candida maltosa
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
60
-
Candida maltosa
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
significant loss of activity above 40°C
Candida maltosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction
Candida maltosa
9.5
-
oxidation
Candida maltosa
Other publictions for EC 1.1.1.222
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286313
Leelayoova
In vitro tryptophan catabolism ...
Leishmania donovani donovani
J. Protozool.
39
350-358
1992
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286312
Bode
-
Purification and properties of ...
Candida maltosa
Biochem. Physiol. Pflanz.
181
189-198
1986
-
-
-
-
-
1
2
9
-
3
3
1
-
1
-
-
1
-
-
-
2
-
8
1
1
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
9
-
3
3
1
-
-
-
1
-
-
2
-
8
1
1
1
1
-
2
-
-
-
-
-
-
-
-
-