BRENDA - Enzyme Database show
show all sequences of 1.1.1.220

The biosynthesis of tetrahydrobiopterin in rat brain. Purification and characterization of 6-pyruvoyl tetrahydropterin (2-oxo)reductase

Milstien, S.; Kaufman, S.; J. Biol. Chem. 264, 8066-8073 (1989)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
6-pyruvoyltetrahydropterin
-
Rattus norvegicus
0.1
-
4-Nitrobenzaldehyde
-
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
SDS-PAGE, gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-pyruvoyltetrahydropterin + NADPH
Rattus norvegicus
involved in de novo biosynthesis of tetrahydrobiopterin
6-lactoyl-5,6,7,8-tetrahydropterin + NADP+
-
Rattus norvegicus
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
from brain
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
highest concentration in striatum and midbrain
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
22
-
crude extract
Rattus norvegicus
49300
-
purified enzyme
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrobenzaldehyde + NADPH
-
286309
Rattus norvegicus
4-nitrobenzylalcohol + NADP+
-
-
-
?
6-pyruvoyltetrahydropterin + NADPH
-
286309
Rattus norvegicus
6-lactoyl-5,6,7,8-tetrahydropterin + NADP+
-
286309
Rattus norvegicus
ir
6-pyruvoyltetrahydropterin + NADPH
involved in de novo biosynthesis of tetrahydrobiopterin
286309
Rattus norvegicus
6-lactoyl-5,6,7,8-tetrahydropterin + NADP+
-
286309
Rattus norvegicus
ir
menadione + NADPH
-
286309
Rattus norvegicus
?
-
-
-
?
Phenanthrenequinone + NADPH
-
286309
Rattus norvegicus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
SDS-PAGE, gel filtration
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
6-pyruvoyltetrahydropterin
-
Rattus norvegicus
0.1
-
4-Nitrobenzaldehyde
-
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
SDS-PAGE, gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-pyruvoyltetrahydropterin + NADPH
Rattus norvegicus
involved in de novo biosynthesis of tetrahydrobiopterin
6-lactoyl-5,6,7,8-tetrahydropterin + NADP+
-
Rattus norvegicus
ir
Purification (Commentary) (protein specific)
Commentary
Organism
from brain
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
highest concentration in striatum and midbrain
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
22
-
crude extract
Rattus norvegicus
49300
-
purified enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrobenzaldehyde + NADPH
-
286309
Rattus norvegicus
4-nitrobenzylalcohol + NADP+
-
-
-
?
6-pyruvoyltetrahydropterin + NADPH
-
286309
Rattus norvegicus
6-lactoyl-5,6,7,8-tetrahydropterin + NADP+
-
286309
Rattus norvegicus
ir
6-pyruvoyltetrahydropterin + NADPH
involved in de novo biosynthesis of tetrahydrobiopterin
286309
Rattus norvegicus
6-lactoyl-5,6,7,8-tetrahydropterin + NADP+
-
286309
Rattus norvegicus
ir
menadione + NADPH
-
286309
Rattus norvegicus
?
-
-
-
?
Phenanthrenequinone + NADPH
-
286309
Rattus norvegicus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
SDS-PAGE, gel filtration
Rattus norvegicus
Other publictions for EC 1.1.1.220
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286309
Milstien
The biosynthesis of tetrahydro ...
Rattus norvegicus
J. Biol. Chem.
264
8066-8073
1989
-
-
-
-
-
-
-
2
-
-
1
1
-
2
-
-
1
-
-
2
2
-
5
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
1
-
-
-
1
-
2
2
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286310
Steinerstauch
Human liver 6-pyruvoyl tetrahy ...
Homo sapiens
Biochem. Biophys. Res. Commun.
164
1130-1136
1989
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286308
Milstien
Biosynthesis of tetrahydrobiop ...
Bos taurus, Rattus norvegicus
Biochem. Biophys. Res. Commun.
128
1099-1107
1985
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
1
-
-
3
3
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
3
3
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-