Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no substrate inhibition, K+ is not inhibitory up to 500 mM, not inhibitory: dimethyldicarbonate, EDTA | Glycine max | |
NADH | 0.1 mM, presence of 0.1 mM NAD+, 44% inhibition, presence of 0.5 mM NAD+, 6% inhibition | Glycine max | |
p-chloromercuribenzoate | 0.001 mM, 66% inhibition | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
UDP-glucose | pH 8.7, 25°C | Glycine max | |
0.07 | - |
NAD+ | pH 8.7, 25°C | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
recombinant enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrate: UDP-galactose, UDP-N-galactosamin, ADP-glucose, GDP-glucose, GDP-mannose | Glycine max | ? | - |
? | |
UDP-glucose + 2 NAD+ + H2O | NADP+, about 1% of activity of NAD+ | Glycine max | UDP-glucuronate + 2 NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
rapid and irreversible inactivation above | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Glycine max |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
p-chloromercuribenzoate | pH 8.7, 25°C | Glycine max |