BRENDA - Enzyme Database show
show all sequences of 1.1.1.219

Molecular characterization and expression analysis of dihydroflavonol 4-reductase (DFR) gene in Saussurea medusa

Li, H.; Qiu, J.; Chen, F.; Lv, X.; Fu, C.; Zhao, D.; Hua, X.; Zhao, Q.; Mol. Biol. Rep. 39, 2991-2999 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae; gene SmDFR, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, functional expression in Saccharomyces cerevisiae strain INV Sc1
Saussurea medusa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38600
-
x * 38600, SDS-PAGE
Saussurea medusa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Saussurea medusa
the enzyme catalyzes the reduction of dihydroflavonols to leucoanthocyanins. But SmDFR also possesses flavanone 4-reductase (FNR) activity and can catalyze the conversion of eridictyol to luteoforol, EC 1.1.1.234
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saussurea medusa
A5Z0G1
gene SmDFR; putative
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
callus
a red callus line
Saussurea medusa
-
flower
; expression in flowers is about 8fold higher compared with leaves and roots
Saussurea medusa
-
leaf
; expression in flowers is about 8fold higher compared with leaves and roots
Saussurea medusa
-
additional information
expression level of SmDFR is higher in flowers compared with both leaves and roots
Saussurea medusa
-
root
; expression in flowers is about 8fold higher compared with leaves and roots
Saussurea medusa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-dihydrokaempferol + NADPH + H+
-
725929
Saussurea medusa
cis-3,4-leucopelargonidin + NADP+
-
-
-
?
(+)-dihydroquercetin + NADPH + H+
-
725929
Saussurea medusa
cis-3,4-leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
725929
Saussurea medusa
leucocyanidin + NADP+
-
-
-
?
additional information
the enzyme catalyzes the reduction of dihydroflavonols to leucoanthocyanins. But SmDFR also possesses flavanone 4-reductase (FNR) activity and can catalyze the conversion of eridictyol to luteoforol, EC 1.1.1.234
725929
Saussurea medusa
?
-
-
-
-
additional information
no activity in reduction of dihydromyricetin
725929
Saussurea medusa
?
-
-
-
-
additional information
no substrate: dihydromyricetin. enzyme additionally shows flavanone-4-reductase activity, EC 1.1.1.234
725929
Saussurea medusa
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 38600, SDS-PAGE
Saussurea medusa
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Saussurea medusa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae; gene SmDFR, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, functional expression in Saccharomyces cerevisiae strain INV Sc1
Saussurea medusa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Saussurea medusa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38600
-
x * 38600, SDS-PAGE
Saussurea medusa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Saussurea medusa
the enzyme catalyzes the reduction of dihydroflavonols to leucoanthocyanins. But SmDFR also possesses flavanone 4-reductase (FNR) activity and can catalyze the conversion of eridictyol to luteoforol, EC 1.1.1.234
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
callus
a red callus line
Saussurea medusa
-
flower
; expression in flowers is about 8fold higher compared with leaves and roots
Saussurea medusa
-
leaf
; expression in flowers is about 8fold higher compared with leaves and roots
Saussurea medusa
-
additional information
expression level of SmDFR is higher in flowers compared with both leaves and roots
Saussurea medusa
-
root
; expression in flowers is about 8fold higher compared with leaves and roots
Saussurea medusa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-dihydrokaempferol + NADPH + H+
-
725929
Saussurea medusa
cis-3,4-leucopelargonidin + NADP+
-
-
-
?
(+)-dihydroquercetin + NADPH + H+
-
725929
Saussurea medusa
cis-3,4-leucocyanidin + NADP+
-
-
-
?
dihydroquercetin + NADPH + H+
-
725929
Saussurea medusa
leucocyanidin + NADP+
-
-
-
?
additional information
the enzyme catalyzes the reduction of dihydroflavonols to leucoanthocyanins. But SmDFR also possesses flavanone 4-reductase (FNR) activity and can catalyze the conversion of eridictyol to luteoforol, EC 1.1.1.234
725929
Saussurea medusa
?
-
-
-
-
additional information
no activity in reduction of dihydromyricetin
725929
Saussurea medusa
?
-
-
-
-
additional information
no substrate: dihydromyricetin. enzyme additionally shows flavanone-4-reductase activity, EC 1.1.1.234
725929
Saussurea medusa
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38600, SDS-PAGE
Saussurea medusa
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the plant DFR superfamily, phylogenetic analysis
Saussurea medusa
metabolism
the DFR gene is a key gene late in the flavonoid biosynthesis pathway, overview. The enzyme is a key enzyme in the biosynthesis of anthocyanidins, proanthocyanidins, and other flavonoids, and also possesses flavanone 4-reductase activity
Saussurea medusa
physiological function
the enzyme is of importance in plant development
Saussurea medusa
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the plant DFR superfamily, phylogenetic analysis
Saussurea medusa
metabolism
the DFR gene is a key gene late in the flavonoid biosynthesis pathway, overview. The enzyme is a key enzyme in the biosynthesis of anthocyanidins, proanthocyanidins, and other flavonoids, and also possesses flavanone 4-reductase activity
Saussurea medusa
physiological function
the enzyme is of importance in plant development
Saussurea medusa
Other publictions for EC 1.1.1.219
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738059
Bulut
Screening, molecular cloning, ...
Komagataella phaffii, Komagataella phaffii GS115
ChemBioChem
17
1349-1358
2016
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15
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1
15
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3
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1
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725929
Li
Molecular characterization and ...
Saussurea medusa
Mol. Biol. Rep.
39
2991-2999
2012
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6
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1
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6
1
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3
3
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726292
Huang
Molecular cloning and characte ...
Populus trichocarpa
PLoS ONE
7
e30364
2012
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1
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6
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3
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5
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1
2
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704148
Cao
Effect of BTH on anthocyanin c ...
Fragaria x ananassa
J. Agric. Food Chem.
58
5801-5805
2010
1
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1
1
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700794
Eungwanichayapant
Accumulation of catechins in t ...
Camellia sinensis var. sinensis
Plant Physiol. Biochem.
47
94-97
2009
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2
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1
1
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702936
Akhov
-
Proanthocyanidin biosynthesis ...
Brassica napus
Botany
87
616-625
2009
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1
1
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705520
Chemler
Improving NADPH availability f ...
Anthurium andraeanum
Metab. Eng.
12
96-104
2009
-
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1
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-
-
-
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2
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1
1
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706155
Singh
-
Characterization of dihydrofla ...
Camellia sinensis, Camellia sinensis (L.) O. Kuntze
Plant Biotechnol. Rep.
3
95-101
2009
-
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1
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2
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1
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2
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3
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1
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1
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2
1
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2
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4
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1
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2
2
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3
2
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2
2
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-
4
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706810
Zhang
The potato R locus codes for d ...
Solanum tuberosum
Theor. Appl. Genet.
119
931-937
2009
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1
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4
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1
1
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713265
Yong
-
Agrobacterium-mediated transfo ...
Torenia fournieri
Plant Cell Tissue Organ Cult.
96
59-67
2009
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1
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713530
Jung
The potato developer (D) locus ...
Solanum tuberosum
Theor. Appl. Genet.
120
45-57
2009
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1
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4
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1
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1
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1
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701213
Nakatsuka
-
Isolation and expression analy ...
Rhododendron x pulchrum
Sci. Hortic.
118
314-320
2008
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1
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1
1
1
1
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701463
Trabelsi
Structural evidence for the in ...
Vitis vinifera
Acta Crystallogr. Sect. D
64
883-891
2008
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1
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2
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702000
Leonard
-
Characterization of dihydrofla ...
Anthurium andraeanum, Arabidopsis thaliana, Fragaria x ananassa, Ipomoea nil, Lilium sp., Rosa hybrid cultivar
Biocatal. Biotransform.
26
243-251
2008
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6
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6
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6
24
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30
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6
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6
6
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24
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30
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1
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1
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6
6
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705358
Lee
-
Molecular cloning and biochemi ...
Brassica rapa subsp. pekinensis
J. Plant Biol.
51
42-47
2008
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1
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688073
Zhou
Ultraviolet A-specific inducti ...
Brassica rapa
J. Exp. Bot.
58
1771-1781
2007
1
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688348
Petit
Crystal structure of grape dih ...
Vitis vinifera
J. Mol. Biol.
368
1345-1357
2007
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1
1
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1
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1
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667201
Takahashi
Evaluation of metabolic altera ...
Oryza sativa
Ann. Bot.
98
819-825
2006
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670520
Lo Piero
Gene characterization, analysi ...
Citrus sinensis
Phytochemistry
67
684-695
2006
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1
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