BRENDA - Enzyme Database show
show all sequences of 1.1.1.219

Proanthocyanidin biosynthesis in the seed coat of yellow-seeded, canola quality Brassica napus YN01-429 is constrained at the committed step catalyzed by dihydroflavonol 4-reductase

Akhov, L.; Ashe, P.; Tan, Y.; Datla, R.; Selvaraj, G.; Botany 87, 616-625 (2009)
No PubMed abstract available

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brassica napus
-
line N89-53 and line YN01-429 (a yellow-seeded canola-quality germplasm)
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed coat
DFR activity is lower in YN01-429 as compared to N89-53 seed coats. Two copies of the DFR gene, which are both functional in YN01-429, homeoallelic repression or silencing, together they show very low expression levels (17fold fewer transcripts) relative to DFR acitvity in N89-53 seed coats
Brassica napus
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed coat
DFR activity is lower in YN01-429 as compared to N89-53 seed coats. Two copies of the DFR gene, which are both functional in YN01-429, homeoallelic repression or silencing, together they show very low expression levels (17fold fewer transcripts) relative to DFR acitvity in N89-53 seed coats
Brassica napus
-
General Information
General Information
Commentary
Organism
physiological function
commits phenolics to proanthocyanidin synthesis
Brassica napus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
commits phenolics to proanthocyanidin synthesis
Brassica napus
Other publictions for EC 1.1.1.219
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Bulut
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Komagataella phaffii, Komagataella phaffii GS115
ChemBioChem
17
1349-1358
2016
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1
15
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-
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725929
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Saussurea medusa
Mol. Biol. Rep.
39
2991-2999
2012
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1
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3
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726292
Huang
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Populus trichocarpa
PLoS ONE
7
e30364
2012
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1
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1
2
-
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-
704148
Cao
Effect of BTH on anthocyanin c ...
Fragaria x ananassa
J. Agric. Food Chem.
58
5801-5805
2010
1
-
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-
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1
1
-
-
-
700794
Eungwanichayapant
Accumulation of catechins in t ...
Camellia sinensis var. sinensis
Plant Physiol. Biochem.
47
94-97
2009
-
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-
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1
1
-
-
-
702936
Akhov
-
Proanthocyanidin biosynthesis ...
Brassica napus
Botany
87
616-625
2009
-
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-
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-
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-
-
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-
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-
-
-
1
1
-
-
-
705520
Chemler
Improving NADPH availability f ...
Anthurium andraeanum
Metab. Eng.
12
96-104
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
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1
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-
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-
-
1
1
-
-
-
706155
Singh
-
Characterization of dihydrofla ...
Camellia sinensis, Camellia sinensis (L.) O. Kuntze
Plant Biotechnol. Rep.
3
95-101
2009
-
-
1
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2
-
1
-
2
-
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3
-
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1
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2
-
-
4
-
-
706810
Zhang
The potato R locus codes for d ...
Solanum tuberosum
Theor. Appl. Genet.
119
931-937
2009
-
-
1
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4
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1
1
-
-
-
713265
Yong
-
Agrobacterium-mediated transfo ...
Torenia fournieri
Plant Cell Tissue Organ Cult.
96
59-67
2009
-
1
-
-
-
-
-
-
-
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-
1
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-
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-
713530
Jung
The potato developer (D) locus ...
Solanum tuberosum
Theor. Appl. Genet.
120
45-57
2009
-
1
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-
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4
-
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-
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1
-
-
701213
Nakatsuka
-
Isolation and expression analy ...
Rhododendron x pulchrum
Sci. Hortic.
118
314-320
2008
-
-
1
-
-
-
-
-
-
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1
1
1
1
-
-
701463
Trabelsi
Structural evidence for the in ...
Vitis vinifera
Acta Crystallogr. Sect. D
64
883-891
2008
-
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1
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2
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4
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-
702000
Leonard
-
Characterization of dihydrofla ...
Anthurium andraeanum, Arabidopsis thaliana, Fragaria x ananassa, Ipomoea nil, Lilium sp., Rosa hybrid cultivar
Biocatal. Biotransform.
26
243-251
2008
-
-
6
-
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-
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6
-
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6
24
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6
24
-
30
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1
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1
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-
-
6
6
-
-
-
705358
Lee
-
Molecular cloning and biochemi ...
Brassica rapa subsp. pekinensis
J. Plant Biol.
51
42-47
2008
-
-
1
-
-
-
-
-
-
-
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-
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1
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-
-
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-
1
1
-
-
-
688073
Zhou
Ultraviolet A-specific inducti ...
Brassica rapa
J. Exp. Bot.
58
1771-1781
2007
1
-
1
-
-
-
-
-
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-
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2
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-
-
688348
Petit
Crystal structure of grape dih ...
Vitis vinifera
J. Mol. Biol.
368
1345-1357
2007
-
-
1
1
1
-
-
-
-
-
-
4
-
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-
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1
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667201
Takahashi
Evaluation of metabolic altera ...
Oryza sativa
Ann. Bot.
98
819-825
2006
-
-
-
-
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2
-
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-
670520
Lo Piero
Gene characterization, analysi ...
Citrus sinensis
Phytochemistry
67
684-695
2006
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
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2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
669664
Shimada
A comprehensive analysis of si ...
Lotus japonicus
J. Exp. Bot.
56
2573-2585
2005
-
-
1
-
-
-
-
-
-
-
5
-
-
4
-
-
-
-
-
1
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-
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1
-
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-
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-
-
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-
-
-
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-
-
-
670686
Hayashi
Enhanced dihydroflavonol-4-red ...
Oryza sativa
Proc. Natl. Acad. Sci. USA
102
7020-7025
2005
-
-
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4
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6
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1
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1
-
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-
-
-
670928
Kim
Development of a PCR-based mar ...
Allium cepa
Theor. Appl. Genet.
110
588-595
2005
-
-
-
-
-
-
-
-
-
-
-
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1
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-
-
-
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-
-
-
657067
Xie
Molecular and biochemical anal ...
Medicago truncatula
Plant Physiol.
134
979-994
2004
-
-
1
-
1
-
-
-
-
-
-
1
-
7
-
-
-
-
-
4
-
-
9
-
1
-
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-
2
-
-
1
-
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-
1
1
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1
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1
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4
-
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9
-
1
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2
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-
655562
Martens
Heterologous expression of dih ...
Callistephus chinensis, Dianthus caryophyllus, Gerbera hybrid cultivar, Matthiola incana, no activity in Escherichia coli, no activity in Nicotiana tabacum, no activity in Saccharomyces cerevisiae, Rosa hybrid cultivar, Rosa hybrid cultivar Kardinal, Solanum lycopersicum
FEBS Lett.
531
453-458
2002
-
-
6
-
-
-
-
-
-
-
-
7
-
10
-
-
-
-
-
18
-
-
29
-
6
-
-
-
6
-
-
6
-
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6
6
-
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7
-
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18
-
-
29
-
6
-
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6
-
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-
-
-
-
-
348000
Singh
-
Biosynthesis of flavan-3-ols b ...
Onobrychis viciifolia
Phytochemistry
44
425-432
1997
-
-
-
-
-
-
2
-
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1
-
1
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1
1
1
4
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1
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2
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2
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1
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1
1
1
4
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1
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1
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347998
Ishikura
-
Conversion of (+)-dihydroquerc ...
Cryptomeria japonica
Plant Cell Physiol.
29
795-799
1988
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3
1
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1
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1
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1
2
3
-
1
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1
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2
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2
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3
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1
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1
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1
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2
1
2
3
-
1
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1
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-
347995
Stafford
Flavan-3-ol biosynthesis. The ...
Ginkgo biloba, Pseudotsuga menziesii
Plant Physiol.
78
791-794
1985
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2
-
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4
1
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4
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2
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2
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4
1
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4
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347996
Heller
Enzymatic reduction of (+)-dih ...
Matthiola incana
Planta
165
284-287
1985
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1
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1
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1
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3
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1
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1
1
1
4
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1
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1
1
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347999
Stafford
Flavan-3-ol biosynthesis. The ...
Pseudotsuga menziesii
Plant Physiol.
76
184-186
1984
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1
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1
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1
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1
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2
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1
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1
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347997
Stafford
Enzymic and nonenzymic reducti ...
Pseudotsuga menziesii
Plant Physiol.
70
695-698
1982
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