BRENDA - Enzyme Database show
show all sequences of 1.1.1.219

Molecular and biochemical analysis of two cDNA clones encoding dihydroflavonol-4-reductase from Medicago truncatula

Xie, D.Y.; Jackson, L.A.; Cooper, J.D.; Ferreira, D.; Paiva, N.L.; Plant Physiol. 134, 979-994 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
isozyme MtDFR1, from a young seed cDNA library, functional expression in Escherichia coli; isozyme MtDFR2, from a young seed cDNA library, functional expression in Escherichia coli
Medicago truncatula
Engineering
Amino acid exchange
Commentary
Organism
additional information
overexpression of the isozyme in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV35S promotor does not lead to increased anthocyanin production in the flowers; overexpression of the isozyme in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV35S promotor leads to increased anthocyanin production in the flowers
Medicago truncatula
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Medicago truncatula
key enzyme in flux control in biosynthetic branched pathways leading to anthocyanins and condensed tannins
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Medicago truncatula
Q6TQT0
isozyme MtDFR2; cv. Jemalong, 2 isozymes MtDFR1 and MtDFR2
-
Medicago truncatula
Q6TQT1
isozyme MtDFR1; cv. Jemalong, 2 isozymes MtDFR1 and MtDFR2
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Medicago truncatula
-
leaf
-
Medicago truncatula
-
additional information
developmental gene expression pattern
Medicago truncatula
-
seed
-
Medicago truncatula
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-2,3-dihydrokaempferol + NADPH
-
657067
Medicago truncatula
cis-3,4-leucopelargonidin + NADP+
-
-
-
?
(+)-2,3-dihydrokaempferol + NADPH
low activity
657067
Medicago truncatula
cis-3,4-leucopelargonidin + NADP+
-
-
-
?
(+/-)-fustin + NADPH
preference for (-)-isomer
657067
Medicago truncatula
?
-
-
-
?
(-)-fustin + NADPH
stereospecific for (-)-isomer
657067
Medicago truncatula
?
-
-
-
?
2,3-dihydromyricetin + NADPH
low activity
657067
Medicago truncatula
cis-3,4-leucodelphinidin + NADP+
-
-
-
?
2,3-dihydroquercetin + NADPH
i.e. (+)-taxifolin, stereospecific for (+)-isomer
657067
Medicago truncatula
cis-3,4-leucocyanidin + NADP+
-
-
-
?
2,3-dihydrorobinetin + NADPH
-
657067
Medicago truncatula
?
-
-
-
?
additional information
key enzyme in flux control in biosynthetic branched pathways leading to anthocyanins and condensed tannins
657067
Medicago truncatula
?
-
-
-
-
additional information
stereospecific enzyme, no activity with substances lacking the the hydroxyl group at the 3-position or with a double bond present between C2 and C3, e.g. quercetin, apigenin, eriodictyol, and kaempferol
657067
Medicago truncatula
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Medicago truncatula
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
-
Medicago truncatula
7
-
-
Medicago truncatula
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Medicago truncatula
Cloned(Commentary) (protein specific)
Commentary
Organism
isozyme MtDFR1, from a young seed cDNA library, functional expression in Escherichia coli; isozyme MtDFR2, from a young seed cDNA library, functional expression in Escherichia coli
Medicago truncatula
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Medicago truncatula
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
overexpression of the isozyme in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV35S promotor does not lead to increased anthocyanin production in the flowers; overexpression of the isozyme in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV35S promotor leads to increased anthocyanin production in the flowers
Medicago truncatula
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Medicago truncatula
key enzyme in flux control in biosynthetic branched pathways leading to anthocyanins and condensed tannins
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Medicago truncatula
-
leaf
-
Medicago truncatula
-
additional information
developmental gene expression pattern
Medicago truncatula
-
seed
-
Medicago truncatula
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-2,3-dihydrokaempferol + NADPH
-
657067
Medicago truncatula
cis-3,4-leucopelargonidin + NADP+
-
-
-
?
(+)-2,3-dihydrokaempferol + NADPH
low activity
657067
Medicago truncatula
cis-3,4-leucopelargonidin + NADP+
-
-
-
?
(+/-)-fustin + NADPH
preference for (-)-isomer
657067
Medicago truncatula
?
-
-
-
?
(-)-fustin + NADPH
stereospecific for (-)-isomer
657067
Medicago truncatula
?
-
-
-
?
2,3-dihydromyricetin + NADPH
low activity
657067
Medicago truncatula
cis-3,4-leucodelphinidin + NADP+
-
-
-
?
2,3-dihydroquercetin + NADPH
i.e. (+)-taxifolin, stereospecific for (+)-isomer
657067
Medicago truncatula
cis-3,4-leucocyanidin + NADP+
-
-
-
?
2,3-dihydrorobinetin + NADPH
-
657067
Medicago truncatula
?
-
-
-
?
additional information
key enzyme in flux control in biosynthetic branched pathways leading to anthocyanins and condensed tannins
657067
Medicago truncatula
?
-
-
-
-
additional information
stereospecific enzyme, no activity with substances lacking the the hydroxyl group at the 3-position or with a double bond present between C2 and C3, e.g. quercetin, apigenin, eriodictyol, and kaempferol
657067
Medicago truncatula
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Medicago truncatula
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
-
Medicago truncatula
7
-
-
Medicago truncatula
Other publictions for EC 1.1.1.219
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738059
Bulut
Screening, molecular cloning, ...
Komagataella phaffii, Komagataella phaffii GS115
ChemBioChem
17
1349-1358
2016
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1
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1
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1
15
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1
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3
1
1
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1
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1
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3
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1
15
1
1
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3
1
1
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725929
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Molecular characterization and ...
Saussurea medusa
Mol. Biol. Rep.
39
2991-2999
2012
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1
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1
1
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6
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5
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6
1
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1
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1
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1
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5
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6
1
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3
3
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726292
Huang
Molecular cloning and characte ...
Populus trichocarpa
PLoS ONE
7
e30364
2012
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1
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6
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5
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1
2
-
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-
704148
Cao
Effect of BTH on anthocyanin c ...
Fragaria x ananassa
J. Agric. Food Chem.
58
5801-5805
2010
1
-
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2
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1
1
1
1
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1
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1
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1
1
-
-
-
700794
Eungwanichayapant
Accumulation of catechins in t ...
Camellia sinensis var. sinensis
Plant Physiol. Biochem.
47
94-97
2009
-
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1
1
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702936
Akhov
-
Proanthocyanidin biosynthesis ...
Brassica napus
Botany
87
616-625
2009
-
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-
-
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1
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1
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-
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-
1
1
-
-
-
705520
Chemler
Improving NADPH availability f ...
Anthurium andraeanum
Metab. Eng.
12
96-104
2009
-
-
1
-
-
-
-
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-
-
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2
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1
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1
1
-
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-
706155
Singh
-
Characterization of dihydrofla ...
Camellia sinensis, Camellia sinensis (L.) O. Kuntze
Plant Biotechnol. Rep.
3
95-101
2009
-
-
1
-
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2
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1
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2
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3
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1
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1
2
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3
1
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1
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2
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2
1
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2
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2
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4
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1
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2
2
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3
2
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-
2
2
-
-
4
-
-
706810
Zhang
The potato R locus codes for d ...
Solanum tuberosum
Theor. Appl. Genet.
119
931-937
2009
-
-
1
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4
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1
1
-
-
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713265
Yong
-
Agrobacterium-mediated transfo ...
Torenia fournieri
Plant Cell Tissue Organ Cult.
96
59-67
2009
-
1
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1
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713530
Jung
The potato developer (D) locus ...
Solanum tuberosum
Theor. Appl. Genet.
120
45-57
2009
-
1
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4
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1
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1
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1
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1
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701213
Nakatsuka
-
Isolation and expression analy ...
Rhododendron x pulchrum
Sci. Hortic.
118
314-320
2008
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1
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4
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1
1
1
1
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-
701463
Trabelsi
Structural evidence for the in ...
Vitis vinifera
Acta Crystallogr. Sect. D
64
883-891
2008
-
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1
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2
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4
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702000
Leonard
-
Characterization of dihydrofla ...
Anthurium andraeanum, Arabidopsis thaliana, Fragaria x ananassa, Ipomoea nil, Lilium sp., Rosa hybrid cultivar
Biocatal. Biotransform.
26
243-251
2008
-
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6
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6
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6
24
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30
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1
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6
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6
6
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6
24
-
30
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1
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1
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6
6
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705358
Lee
-
Molecular cloning and biochemi ...
Brassica rapa subsp. pekinensis
J. Plant Biol.
51
42-47
2008
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1
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688073
Zhou
Ultraviolet A-specific inducti ...
Brassica rapa
J. Exp. Bot.
58
1771-1781
2007
1
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1
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688348
Petit
Crystal structure of grape dih ...
Vitis vinifera
J. Mol. Biol.
368
1345-1357
2007
-
-
1
1
1
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4
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5
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1
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5
1
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2
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1
2
1
1
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1
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5
1
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667201
Takahashi
Evaluation of metabolic altera ...
Oryza sativa
Ann. Bot.
98
819-825
2006
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2
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670520
Lo Piero
Gene characterization, analysi ...
Citrus sinensis
Phytochemistry
67
684-695
2006
-
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1
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2
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1
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