BRENDA - Enzyme Database show
show all sequences of 1.1.1.218

Mechanistic studies of morphine dehydrogenase and stabilization against covalent inactivation

Walker, E.H.; French, C.E.; Rathbone, D.A.; Bruce, N.C.; Biochem. J. 345, 687-692 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene encoding morphine dehydrogenase on natural plasmid, expressed in Escherichia coli
Pseudomonas putida
Engineering
Amino acid exchange
Commentary
Organism
C80S
change of cysteine to serine: significantly improved stability
Pseudomonas putida
D47N
residue modified by site-directed mutagenesis, resulting in substantial loss of activity
Pseudomonas putida
K76M
residue modified by site-directed mutagenesis, resulting in substantial loss of activity
Pseudomonas putida
Y52F
residue modified by site-directed mutagenesis, resulting in substantial loss of activity
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
competitive inhibition: NADP+ with respect to NADPH, codeine with respect to codeinone
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
codeinone
cosubstrate NADPH
Pseudomonas putida
0.45
-
codeine
cosubstrate NADP+
Pseudomonas putida
0.54
-
morphine
cosubstrate NADP+
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31990
-
based on sequence data
Pseudomonas putida
32000
-
elecrospray mass spectrometer
Pseudomonas putida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
M10
-
Pseudomonas putida M10
-
M10
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
codeine + NAD(P)+
non-Theorell-Chance sequential ordered mechanism for codeine oxidation at pH 9.5
286289
Pseudomonas putida
codeinone + NAD(P)H
morphine dehydrogenase follows a Theorell-Chance mechanism for codeinone reduction at pH 7.0
286289
Pseudomonas putida
-
codeine + NAD(P)+
non-Theorell-Chance sequential ordered mechanism for codeine oxidation at pH 9.5
286289
Pseudomonas putida M10
codeinone + NAD(P)H
morphine dehydrogenase follows a Theorell-Chance mechanism for codeinone reduction at pH 7.0
286289
Pseudomonas putida M10
-
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)H
enzyme specific for the proR hydrogen of NADPH
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
gene encoding morphine dehydrogenase on natural plasmid, expressed in Escherichia coli
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)H
enzyme specific for the proR hydrogen of NADPH
Pseudomonas putida
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C80S
change of cysteine to serine: significantly improved stability
Pseudomonas putida
D47N
residue modified by site-directed mutagenesis, resulting in substantial loss of activity
Pseudomonas putida
K76M
residue modified by site-directed mutagenesis, resulting in substantial loss of activity
Pseudomonas putida
Y52F
residue modified by site-directed mutagenesis, resulting in substantial loss of activity
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
competitive inhibition: NADP+ with respect to NADPH, codeine with respect to codeinone
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
codeinone
cosubstrate NADPH
Pseudomonas putida
0.45
-
codeine
cosubstrate NADP+
Pseudomonas putida
0.54
-
morphine
cosubstrate NADP+
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31990
-
based on sequence data
Pseudomonas putida
32000
-
elecrospray mass spectrometer
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
codeine + NAD(P)+
non-Theorell-Chance sequential ordered mechanism for codeine oxidation at pH 9.5
286289
Pseudomonas putida
codeinone + NAD(P)H
morphine dehydrogenase follows a Theorell-Chance mechanism for codeinone reduction at pH 7.0
286289
Pseudomonas putida
-
codeine + NAD(P)+
non-Theorell-Chance sequential ordered mechanism for codeine oxidation at pH 9.5
286289
Pseudomonas putida M10
codeinone + NAD(P)H
morphine dehydrogenase follows a Theorell-Chance mechanism for codeinone reduction at pH 7.0
286289
Pseudomonas putida M10
-
Other publictions for EC 1.1.1.218
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
667890
Todaka
Bioactivation of morphine in h ...
Homo sapiens
Biol. Pharm. Bull.
28
1275-1280
2005
-
-
-
-
-
-
14
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
14
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286288
Todaka
Purification and characterizat ...
Mesocricetus auratus
Arch. Biochem. Biophys.
374
189-197
2000
-
-
-
-
-
-
7
3
1
-
2
-
-
1
-
-
1
-
-
1
1
1
3
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
3
1
-
2
-
-
-
-
1
-
1
1
1
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286289
Walker
Mechanistic studies of morphin ...
Pseudomonas putida, Pseudomonas putida M10
Biochem. J.
345
687-692
2000
-
-
1
-
4
-
1
3
-
-
2
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
4
-
-
1
-
3
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286286
Yamano
Purification, characterization ...
Oryctolagus cuniculus
Arch. Biochem. Biophys.
341
81-88
1997
-
-
-
-
-
1
6
9
3
-
3
-
-
2
-
-
1
-
-
2
1
1
9
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
1
-
6
-
9
3
-
3
-
-
-
-
1
-
2
1
1
9
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286287
Yamano
In vivo and in vitro formation ...
Rattus norvegicus, Rattus norvegicus Wistar
Xenobiotica
27
645-656
1997
-
-
-
-
-
-
7
2
1
-
-
-
-
167
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
2
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286285
Walker
Towards engineering an improve ...
Pseudomonas putida, Pseudomonas putida M10
Ann. N. Y. Acad. Sci.
799
6-10
1996
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286284
Bruce
-
Microbial degradation of the m ...
Pseudomonas putida, Pseudomonas putida M10
Biochem. J.
274
875-880
1991
-
-
-
-
-
2
6
4
-
-
3
2
-
5
-
-
1
-
-
-
1
1
8
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
2
-
6
-
4
-
-
3
2
-
-
-
1
-
-
1
1
8
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286283
Bruce
Microbial degradation of the m ...
Pseudomonas putida, Pseudomonas putida M10
Arch. Microbiol.
154
465-470
1990
-
-
-
-
-
1
-
-
-
-
-
4
-
5
-
-
1
-
-
-
1
-
8
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
4
-
-
-
1
-
-
1
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286282
Yamano
Guinea-pig liver morphine 6-de ...
Cavia porcellus, Cavia porcellus Hartley
Biochem. Pharmacol.
35
4321-4326
1986
-
-
-
-
-
-
7
4
1
-
-
-
-
12
-
-
1
-
-
3
2
2
4
-
1
-
-
-
2
-
1
2
-
-
-
-
-
-
2
-
-
-
-
7
-
4
1
-
-
-
-
-
-
1
-
3
2
2
4
-
1
-
-
-
2
-
1
-
-
-
-
-
-
-
286281
Yamano
Purification and characterizat ...
Cavia porcellus, Cavia porcellus Hartley
J. Biol. Chem.
260
5259-5264
1985
1
-
-
-
-
1
3
12
1
-
1
-
-
12
-
-
1
1
-
2
1
1
12
-
1
-
-
-
2
-
2
1
-
-
-
1
-
-
1
-
-
1
-
3
-
12
1
-
1
-
-
-
-
1
-
2
1
1
12
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-