Cloned (Comment) | Organism |
---|---|
gene encoding morphine dehydrogenase on natural plasmid, expressed in Escherichia coli | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
C80S | change of cysteine to serine: significantly improved stability | Pseudomonas putida |
D47N | residue modified by site-directed mutagenesis, resulting in substantial loss of activity | Pseudomonas putida |
K76M | residue modified by site-directed mutagenesis, resulting in substantial loss of activity | Pseudomonas putida |
Y52F | residue modified by site-directed mutagenesis, resulting in substantial loss of activity | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | competitive inhibition: NADP+ with respect to NADPH, codeine with respect to codeinone | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
codeinone | cosubstrate NADPH | Pseudomonas putida | |
0.45 | - |
codeine | cosubstrate NADP+ | Pseudomonas putida | |
0.54 | - |
morphine | cosubstrate NADP+ | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31990 | - |
based on sequence data | Pseudomonas putida |
32000 | - |
elecrospray mass spectrometer | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
M10 | - |
Pseudomonas putida M10 | - |
M10 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
codeine + NAD(P)+ | non-Theorell-Chance sequential ordered mechanism for codeine oxidation at pH 9.5 | Pseudomonas putida | codeinone + NAD(P)H | morphine dehydrogenase follows a Theorell-Chance mechanism for codeinone reduction at pH 7.0 | ? | |
codeine + NAD(P)+ | non-Theorell-Chance sequential ordered mechanism for codeine oxidation at pH 9.5 | Pseudomonas putida M10 | codeinone + NAD(P)H | morphine dehydrogenase follows a Theorell-Chance mechanism for codeinone reduction at pH 7.0 | ? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | enzyme specific for the proR hydrogen of NADPH | Pseudomonas putida |