BRENDA - Enzyme Database show
show all sequences of 1.1.1.218

Purification and characterization of NAD-dependent morphine 6-dehydrogenase from hamster liver cytosol, a new member of the aldo-keto reductase superfamily

Todaka, T.; Yamano, S.; Toki, S.; Arch. Biochem. Biophys. 374, 189-197 (2000)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ketamine
-
Mesocricetus auratus
lithocholic acid
moderate inhibitory effect
Mesocricetus auratus
additional information
competitive inhibitors: testosterone, 5alpha-androstan-17beta-ol-3-one, 5beta-androsteran-17beta-ol-3-one; no inhibition by indomethacine; no or weak inhibition by pyrazole, barbital
Mesocricetus auratus
NEM
-
Mesocricetus auratus
p-chloromercuribenzoic acid
-
Mesocricetus auratus
Phenylarsine oxide
-
Mesocricetus auratus
quercetin
weak inhibitory effect
Mesocricetus auratus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
5alpha-androstan-17beta-ol-3-one
similar values of other 17beta-hydroxysteroids
Mesocricetus auratus
0.009
-
testosterone
cosubstrate NAD+
Mesocricetus auratus
1.25
-
morphine
cosubstrate NAD+
Mesocricetus auratus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mesocricetus auratus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
native enzyme, gel filtration
Mesocricetus auratus
38000
-
denatured enzyme, SDS-PAGE
Mesocricetus auratus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mesocricetus auratus
-
Syrian hamster
-
Purification (Commentary)
Commentary
Organism
gel filtration, various chromatographic techniques
Mesocricetus auratus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Mesocricetus auratus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.21
-
morphine, NAD+
Mesocricetus auratus
Storage Stability
Storage Stability
Organism
-80°C, 20% glycerol, v/v, stable at least four months
Mesocricetus auratus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
codeine + NAD(P)+
only weak enzyme activity for codeine
286288
Mesocricetus auratus
codeinone + NAD(P)H
-
-
-
?
morphine + NADP+
enzyme activity only with NAD+
286288
Mesocricetus auratus
morphinone + NADPH + H+
-
-
-
?
normorphine + NAD(P)+
best substrate, oxidation at 139% the rate of morphine oxidation
286288
Mesocricetus auratus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE
Mesocricetus auratus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
enzyme assay
Mesocricetus auratus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
in glycine-NaCl-NaOH buffer
Mesocricetus auratus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
with NADP+ enzyme activity less than 5% those found with NAD+
Mesocricetus auratus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
with NADP+ enzyme activity less than 5% those found with NAD+
Mesocricetus auratus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ketamine
-
Mesocricetus auratus
lithocholic acid
moderate inhibitory effect
Mesocricetus auratus
additional information
competitive inhibitors: testosterone, 5alpha-androstan-17beta-ol-3-one, 5beta-androsteran-17beta-ol-3-one; no inhibition by indomethacine; no or weak inhibition by pyrazole, barbital
Mesocricetus auratus
NEM
-
Mesocricetus auratus
p-chloromercuribenzoic acid
-
Mesocricetus auratus
Phenylarsine oxide
-
Mesocricetus auratus
quercetin
weak inhibitory effect
Mesocricetus auratus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
5alpha-androstan-17beta-ol-3-one
similar values of other 17beta-hydroxysteroids
Mesocricetus auratus
0.009
-
testosterone
cosubstrate NAD+
Mesocricetus auratus
1.25
-
morphine
cosubstrate NAD+
Mesocricetus auratus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mesocricetus auratus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
native enzyme, gel filtration
Mesocricetus auratus
38000
-
denatured enzyme, SDS-PAGE
Mesocricetus auratus
Purification (Commentary) (protein specific)
Commentary
Organism
gel filtration, various chromatographic techniques
Mesocricetus auratus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Mesocricetus auratus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.21
-
morphine, NAD+
Mesocricetus auratus
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, 20% glycerol, v/v, stable at least four months
Mesocricetus auratus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
codeine + NAD(P)+
only weak enzyme activity for codeine
286288
Mesocricetus auratus
codeinone + NAD(P)H
-
-
-
?
morphine + NADP+
enzyme activity only with NAD+
286288
Mesocricetus auratus
morphinone + NADPH + H+
-
-
-
?
normorphine + NAD(P)+
best substrate, oxidation at 139% the rate of morphine oxidation
286288
Mesocricetus auratus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE
Mesocricetus auratus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
enzyme assay
Mesocricetus auratus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
in glycine-NaCl-NaOH buffer
Mesocricetus auratus
Other publictions for EC 1.1.1.218
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
667890
Todaka
Bioactivation of morphine in h ...
Homo sapiens
Biol. Pharm. Bull.
28
1275-1280
2005
-
-
-
-
-
-
14
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
14
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286288
Todaka
Purification and characterizat ...
Mesocricetus auratus
Arch. Biochem. Biophys.
374
189-197
2000
-
-
-
-
-
-
7
3
1
-
2
-
-
1
-
-
1
-
-
1
1
1
3
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
3
1
-
2
-
-
-
-
1
-
1
1
1
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286289
Walker
Mechanistic studies of morphin ...
Pseudomonas putida, Pseudomonas putida M10
Biochem. J.
345
687-692
2000
-
-
1
-
4
-
1
3
-
-
2
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
4
-
-
1
-
3
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286286
Yamano
Purification, characterization ...
Oryctolagus cuniculus
Arch. Biochem. Biophys.
341
81-88
1997
-
-
-
-
-
1
6
9
3
-
3
-
-
2
-
-
1
-
-
2
1
1
9
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
1
-
6
-
9
3
-
3
-
-
-
-
1
-
2
1
1
9
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286287
Yamano
In vivo and in vitro formation ...
Rattus norvegicus, Rattus norvegicus Wistar
Xenobiotica
27
645-656
1997
-
-
-
-
-
-
7
2
1
-
-
-
-
167
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
2
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286285
Walker
Towards engineering an improve ...
Pseudomonas putida, Pseudomonas putida M10
Ann. N. Y. Acad. Sci.
799
6-10
1996
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286284
Bruce
-
Microbial degradation of the m ...
Pseudomonas putida, Pseudomonas putida M10
Biochem. J.
274
875-880
1991
-
-
-
-
-
2
6
4
-
-
3
2
-
5
-
-
1
-
-
-
1
1
8
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
2
-
6
-
4
-
-
3
2
-
-
-
1
-
-
1
1
8
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286283
Bruce
Microbial degradation of the m ...
Pseudomonas putida, Pseudomonas putida M10
Arch. Microbiol.
154
465-470
1990
-
-
-
-
-
1
-
-
-
-
-
4
-
5
-
-
1
-
-
-
1
-
8
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
4
-
-
-
1
-
-
1
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286282
Yamano
Guinea-pig liver morphine 6-de ...
Cavia porcellus, Cavia porcellus Hartley
Biochem. Pharmacol.
35
4321-4326
1986
-
-
-
-
-
-
7
4
1
-
-
-
-
12
-
-
1
-
-
3
2
2
4
-
1
-
-
-
2
-
1
2
-
-
-
-
-
-
2
-
-
-
-
7
-
4
1
-
-
-
-
-
-
1
-
3
2
2
4
-
1
-
-
-
2
-
1
-
-
-
-
-
-
-
286281
Yamano
Purification and characterizat ...
Cavia porcellus, Cavia porcellus Hartley
J. Biol. Chem.
260
5259-5264
1985
1
-
-
-
-
1
3
12
1
-
1
-
-
12
-
-
1
1
-
2
1
1
12
-
1
-
-
-
2
-
2
1
-
-
-
1
-
-
1
-
-
1
-
3
-
12
1
-
1
-
-
-
-
1
-
2
1
1
12
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-