BRENDA - Enzyme Database show
show all sequences of 1.1.1.218

In vivo and in vitro formation of morphinone from morphine in rat

Yamano, S.; Takahashi, A.; Todaka, T.; Toki, S.; Xenobiotica 27, 645-656 (1997)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5alpha-dihydrotestosterone
-
Rattus norvegicus
5beta-dihydrotestosterone
-
Rattus norvegicus
androsterone
-
Rattus norvegicus
estradiol
-
Rattus norvegicus
additional information
no or weak inhibition by pyrazole, barbital
Rattus norvegicus
quercetin
-
Rattus norvegicus
testosterone
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.9
-
morphine
cosubstrate NAD+, in the male adult rats
Rattus norvegicus
3.3
-
morphine
cosubstrate NAD+, in the female adult rats
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
microsomal fraction
Rattus norvegicus
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
Wistar
-
Rattus norvegicus Wistar
-
Wistar
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
bile
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
morphine + NADP+
+ NAD+: enzyme activity ten times that with NADP+
286287
Rattus norvegicus
morphinone + NADPH + H+
-
286287
Rattus norvegicus
?
morphine + NADP+
+ NAD+: enzyme activity ten times that with NADP+
286287
Rattus norvegicus Wistar
morphinone + NADPH + H+
-
286287
Rattus norvegicus Wistar
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
in different buffers
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
NADP+: only about one-tenth of activity with NAD+
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
NADP+: only about one-tenth of activity with NAD+
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5alpha-dihydrotestosterone
-
Rattus norvegicus
5beta-dihydrotestosterone
-
Rattus norvegicus
androsterone
-
Rattus norvegicus
estradiol
-
Rattus norvegicus
additional information
no or weak inhibition by pyrazole, barbital
Rattus norvegicus
quercetin
-
Rattus norvegicus
testosterone
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.9
-
morphine
cosubstrate NAD+, in the male adult rats
Rattus norvegicus
3.3
-
morphine
cosubstrate NAD+, in the female adult rats
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
microsomal fraction
Rattus norvegicus
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
bile
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
morphine + NADP+
+ NAD+: enzyme activity ten times that with NADP+
286287
Rattus norvegicus
morphinone + NADPH + H+
-
286287
Rattus norvegicus
?
morphine + NADP+
+ NAD+: enzyme activity ten times that with NADP+
286287
Rattus norvegicus Wistar
morphinone + NADPH + H+
-
286287
Rattus norvegicus Wistar
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
in different buffers
Rattus norvegicus
Other publictions for EC 1.1.1.218
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
667890
Todaka
Bioactivation of morphine in h ...
Homo sapiens
Biol. Pharm. Bull.
28
1275-1280
2005
-
-
-
-
-
-
14
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
14
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286288
Todaka
Purification and characterizat ...
Mesocricetus auratus
Arch. Biochem. Biophys.
374
189-197
2000
-
-
-
-
-
-
7
3
1
-
2
-
-
1
-
-
1
-
-
1
1
1
3
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
3
1
-
2
-
-
-
-
1
-
1
1
1
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286289
Walker
Mechanistic studies of morphin ...
Pseudomonas putida, Pseudomonas putida M10
Biochem. J.
345
687-692
2000
-
-
1
-
4
-
1
3
-
-
2
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
4
-
-
1
-
3
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286286
Yamano
Purification, characterization ...
Oryctolagus cuniculus
Arch. Biochem. Biophys.
341
81-88
1997
-
-
-
-
-
1
6
9
3
-
3
-
-
2
-
-
1
-
-
2
1
1
9
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
1
-
6
-
9
3
-
3
-
-
-
-
1
-
2
1
1
9
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286287
Yamano
In vivo and in vitro formation ...
Rattus norvegicus, Rattus norvegicus Wistar
Xenobiotica
27
645-656
1997
-
-
-
-
-
-
7
2
1
-
-
-
-
167
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
2
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286285
Walker
Towards engineering an improve ...
Pseudomonas putida, Pseudomonas putida M10
Ann. N. Y. Acad. Sci.
799
6-10
1996
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286284
Bruce
-
Microbial degradation of the m ...
Pseudomonas putida, Pseudomonas putida M10
Biochem. J.
274
875-880
1991
-
-
-
-
-
2
6
4
-
-
3
2
-
5
-
-
1
-
-
-
1
1
8
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
2
-
6
-
4
-
-
3
2
-
-
-
1
-
-
1
1
8
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286283
Bruce
Microbial degradation of the m ...
Pseudomonas putida, Pseudomonas putida M10
Arch. Microbiol.
154
465-470
1990
-
-
-
-
-
1
-
-
-
-
-
4
-
5
-
-
1
-
-
-
1
-
8
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
4
-
-
-
1
-
-
1
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286282
Yamano
Guinea-pig liver morphine 6-de ...
Cavia porcellus, Cavia porcellus Hartley
Biochem. Pharmacol.
35
4321-4326
1986
-
-
-
-
-
-
7
4
1
-
-
-
-
12
-
-
1
-
-
3
2
2
4
-
1
-
-
-
2
-
1
2
-
-
-
-
-
-
2
-
-
-
-
7
-
4
1
-
-
-
-
-
-
1
-
3
2
2
4
-
1
-
-
-
2
-
1
-
-
-
-
-
-
-
286281
Yamano
Purification and characterizat ...
Cavia porcellus, Cavia porcellus Hartley
J. Biol. Chem.
260
5259-5264
1985
1
-
-
-
-
1
3
12
1
-
1
-
-
12
-
-
1
1
-
2
1
1
12
-
1
-
-
-
2
-
2
1
-
-
-
1
-
-
1
-
-
1
-
3
-
12
1
-
1
-
-
-
-
1
-
2
1
1
12
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-