BRENDA - Enzyme Database show
show all sequences of 1.1.1.218

Purification, characterization and partial primary structure of morphine 6-dehydrogenase from rabbit liver cytosol

Yamano, S.; Ito, K.; Ogata, S.; Toki, S.; Arch. Biochem. Biophys. 341, 81-88 (1997)

Data extracted from this reference:

General Stability
General Stability
Organism
enzyme stable without 2-mercaptoethanol
Oryctolagus cuniculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
5beta-dihydrotestosterone
-
Oryctolagus cuniculus
additional information
5beta-dihydrotestosterone, lithocholic acid, androsterone and estradiol inhibit competitively toward morphine binding; little inhibitory effect: indomethacin, prostaglandins E2, D2; no or weak inhibition by pyrazole, barbital
Oryctolagus cuniculus
NEM
-
Oryctolagus cuniculus
p-chloromercuribenzoic acid
-
Oryctolagus cuniculus
Phenylarsine oxide
-
Oryctolagus cuniculus
quercetin
-
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
codeine
with NAD+
Oryctolagus cuniculus
0.12
-
1-Indanol
cosubstrate NAD+
Oryctolagus cuniculus
0.39
-
Dihydrocodeine
cosubstrate NAD+
Oryctolagus cuniculus
0.54
-
cyclohexene-2-ol
cosubstrate NAD+
Oryctolagus cuniculus
0.64
-
normorphine
cosubstrate NAD+
Oryctolagus cuniculus
0.72
-
morphine
cosubstrate NAD+
Oryctolagus cuniculus
0.92
-
3-hydroxyhexobarbital
cosubstrate NAD+
Oryctolagus cuniculus
1.48
-
ethylmorphine
cosubstrate NAD+
Oryctolagus cuniculus
1.75
-
benzenedihydrodiol
cosubstrate NAD+
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryctolagus cuniculus
5829
-
microsome
microsomal fraction
Oryctolagus cuniculus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
native enzyme, gel filtration
Oryctolagus cuniculus
36000
-
denatured enzyme, SDS-PAGE
Oryctolagus cuniculus
38000
-
1 * 38000, SDS-PAGE
Oryctolagus cuniculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
Japanese white rabbit
-
Purification (Commentary)
Commentary
Organism
gel filtration, various chromatographic techniques
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.43
-
morphine, NAD+
Oryctolagus cuniculus
Storage Stability
Storage Stability
Organism
-80°C, 20% glycerol, v/v, stable at least four months
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-indanol + NADP+
-
286286
Oryctolagus cuniculus
1-indanone + NADPH
-
-
-
?
3-hydroxyhexobarbital + NADP+
does not serve as a substrate for guinea pig enzyme
286286
Oryctolagus cuniculus
?
-
-
-
?
benzenedihydrodiol + NADP+
-
286286
Oryctolagus cuniculus
?
-
-
-
?
codeine + NAD(P)+
codeine best substrate
286286
Oryctolagus cuniculus
codeinone + NAD(P)H
-
-
-
?
cyclohexene-2-ol + NADP+
-
286286
Oryctolagus cuniculus
cyclohexene-2-one + NADPH
-
-
-
?
dihydrocodeine + NADP+
-
286286
Oryctolagus cuniculus
dihydrocodeinone + NADPH
-
-
-
-
ethylmorphine + NAD(P)+
-
286286
Oryctolagus cuniculus
?
-
-
-
?
morphine + NADP+
enzyme acitivity with NAD+ four times that with NADP+
286286
Oryctolagus cuniculus
morphinone + NADPH + H+
-
-
-
?
normorphine + NAD(P)+
-
286286
Oryctolagus cuniculus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE
Oryctolagus cuniculus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
enzyme assay
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
with NADP+ as cofactor, in glycine-NaCl-NaOH buffer
Oryctolagus cuniculus
9.4
-
with NAD+ as cofactor, in glycine-NaCl-NaOH buffer
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)+
NAD+ preferred cofactor
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)+
NAD+ preferred cofactor
Oryctolagus cuniculus
General Stability (protein specific)
General Stability
Organism
enzyme stable without 2-mercaptoethanol
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5beta-dihydrotestosterone
-
Oryctolagus cuniculus
additional information
5beta-dihydrotestosterone, lithocholic acid, androsterone and estradiol inhibit competitively toward morphine binding; little inhibitory effect: indomethacin, prostaglandins E2, D2; no or weak inhibition by pyrazole, barbital
Oryctolagus cuniculus
NEM
-
Oryctolagus cuniculus
p-chloromercuribenzoic acid
-
Oryctolagus cuniculus
Phenylarsine oxide
-
Oryctolagus cuniculus
quercetin
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
codeine
with NAD+
Oryctolagus cuniculus
0.12
-
1-Indanol
cosubstrate NAD+
Oryctolagus cuniculus
0.39
-
Dihydrocodeine
cosubstrate NAD+
Oryctolagus cuniculus
0.54
-
cyclohexene-2-ol
cosubstrate NAD+
Oryctolagus cuniculus
0.64
-
normorphine
cosubstrate NAD+
Oryctolagus cuniculus
0.72
-
morphine
cosubstrate NAD+
Oryctolagus cuniculus
0.92
-
3-hydroxyhexobarbital
cosubstrate NAD+
Oryctolagus cuniculus
1.48
-
ethylmorphine
cosubstrate NAD+
Oryctolagus cuniculus
1.75
-
benzenedihydrodiol
cosubstrate NAD+
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryctolagus cuniculus
5829
-
microsome
microsomal fraction
Oryctolagus cuniculus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
native enzyme, gel filtration
Oryctolagus cuniculus
36000
-
denatured enzyme, SDS-PAGE
Oryctolagus cuniculus
38000
-
1 * 38000, SDS-PAGE
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
Commentary
Organism
gel filtration, various chromatographic techniques
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.43
-
morphine, NAD+
Oryctolagus cuniculus
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, 20% glycerol, v/v, stable at least four months
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-indanol + NADP+
-
286286
Oryctolagus cuniculus
1-indanone + NADPH
-
-
-
?
3-hydroxyhexobarbital + NADP+
does not serve as a substrate for guinea pig enzyme
286286
Oryctolagus cuniculus
?
-
-
-
?
benzenedihydrodiol + NADP+
-
286286
Oryctolagus cuniculus
?
-
-
-
?
codeine + NAD(P)+
codeine best substrate
286286
Oryctolagus cuniculus
codeinone + NAD(P)H
-
-
-
?
cyclohexene-2-ol + NADP+
-
286286
Oryctolagus cuniculus
cyclohexene-2-one + NADPH
-
-
-
?
dihydrocodeine + NADP+
-
286286
Oryctolagus cuniculus
dihydrocodeinone + NADPH
-
-
-
-
ethylmorphine + NAD(P)+
-
286286
Oryctolagus cuniculus
?
-
-
-
?
morphine + NADP+
enzyme acitivity with NAD+ four times that with NADP+
286286
Oryctolagus cuniculus
morphinone + NADPH + H+
-
-
-
?
normorphine + NAD(P)+
-
286286
Oryctolagus cuniculus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE
Oryctolagus cuniculus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
enzyme assay
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
with NADP+ as cofactor, in glycine-NaCl-NaOH buffer
Oryctolagus cuniculus
9.4
-
with NAD+ as cofactor, in glycine-NaCl-NaOH buffer
Oryctolagus cuniculus
Other publictions for EC 1.1.1.218
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
667890
Todaka
Bioactivation of morphine in h ...
Homo sapiens
Biol. Pharm. Bull.
28
1275-1280
2005
-
-
-
-
-
-
14
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
14
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286288
Todaka
Purification and characterizat ...
Mesocricetus auratus
Arch. Biochem. Biophys.
374
189-197
2000
-
-
-
-
-
-
7
3
1
-
2
-
-
1
-
-
1
-
-
1
1
1
3
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
3
1
-
2
-
-
-
-
1
-
1
1
1
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286289
Walker
Mechanistic studies of morphin ...
Pseudomonas putida, Pseudomonas putida M10
Biochem. J.
345
687-692
2000
-
-
1
-
4
-
1
3
-
-
2
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
4
-
-
1
-
3
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286286
Yamano
Purification, characterization ...
Oryctolagus cuniculus
Arch. Biochem. Biophys.
341
81-88
1997
-
-
-
-
-
1
6
9
3
-
3
-
-
2
-
-
1
-
-
2
1
1
9
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
1
-
6
-
9
3
-
3
-
-
-
-
1
-
2
1
1
9
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286287
Yamano
In vivo and in vitro formation ...
Rattus norvegicus, Rattus norvegicus Wistar
Xenobiotica
27
645-656
1997
-
-
-
-
-
-
7
2
1
-
-
-
-
167
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
2
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286285
Walker
Towards engineering an improve ...
Pseudomonas putida, Pseudomonas putida M10
Ann. N. Y. Acad. Sci.
799
6-10
1996
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286284
Bruce
-
Microbial degradation of the m ...
Pseudomonas putida, Pseudomonas putida M10
Biochem. J.
274
875-880
1991
-
-
-
-
-
2
6
4
-
-
3
2
-
5
-
-
1
-
-
-
1
1
8
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
2
-
6
-
4
-
-
3
2
-
-
-
1
-
-
1
1
8
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286283
Bruce
Microbial degradation of the m ...
Pseudomonas putida, Pseudomonas putida M10
Arch. Microbiol.
154
465-470
1990
-
-
-
-
-
1
-
-
-
-
-
4
-
5
-
-
1
-
-
-
1
-
8
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
4
-
-
-
1
-
-
1
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286282
Yamano
Guinea-pig liver morphine 6-de ...
Cavia porcellus, Cavia porcellus Hartley
Biochem. Pharmacol.
35
4321-4326
1986
-
-
-
-
-
-
7
4
1
-
-
-
-
12
-
-
1
-
-
3
2
2
4
-
1
-
-
-
2
-
1
2
-
-
-
-
-
-
2
-
-
-
-
7
-
4
1
-
-
-
-
-
-
1
-
3
2
2
4
-
1
-
-
-
2
-
1
-
-
-
-
-
-
-
286281
Yamano
Purification and characterizat ...
Cavia porcellus, Cavia porcellus Hartley
J. Biol. Chem.
260
5259-5264
1985
1
-
-
-
-
1
3
12
1
-
1
-
-
12
-
-
1
1
-
2
1
1
12
-
1
-
-
-
2
-
2
1
-
-
-
1
-
-
1
-
-
1
-
3
-
12
1
-
1
-
-
-
-
1
-
2
1
1
12
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-