BRENDA - Enzyme Database show
show all sequences of 1.1.1.218

Microbial degradation of the morphine alkaloids

Bruce, N.C.; Wilmot, C.J.; Jordan, K.N.; Stephens, L.D.G.; Lowe, Ch.R.; Biochem. J. 274, 875-880 (1991)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
2-mercaptoethanol stabilizes
Pseudomonas putida
dithiothreitol stabilizes
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Pseudomonas putida
2,2'-dipyridyl
-
Pseudomonas putida
CuSO4
-
Pseudomonas putida
iodoacetate
-
Pseudomonas putida
additional information
no inhibition by EDTA, NEM, hydroxyquinoline
Pseudomonas putida
NEM
inhibit activity at much higher concentration (e.g. at 1 mM)
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.044
-
codeine
with NADP+
Pseudomonas putida
0.35
-
NADP+
-
Pseudomonas putida
0.46
-
morphine
cosubstrate NADP+
Pseudomonas putida
2.91
-
Dihydrocodeine
cosubstrate NADP+
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
denatured enzyme, SDS-PAGE
Pseudomonas putida
32000
-
native enzyme, gel filtration
Pseudomonas putida
310000
-
1 * 310000, SDS-PAGE
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
morphine + NADP+
Pseudomonas putida
highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source
morphinone + NADPH
-
-
?
morphine + NADP+
Pseudomonas putida M10
highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source
morphinone + NADPH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
M10
-
Pseudomonas putida M10
-
M10
-
Purification (Commentary)
Commentary
Organism
gel filtration, various chromatographic techniques
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.08
-
morphine, NADP+
Pseudomonas putida
Storage Stability
Storage Stability
Organism
-80°C, 10% loss of activity over 2 months
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
codeine + NAD(P)+
oxidation at 120% the rate of morphine oxidation
286284
Pseudomonas putida
codeinone + NAD(P)H
at pH 6.5, + NADPH, reduction of codeinone to codeine
286284
Pseudomonas putida
r
codeine + NAD(P)+
oxidation at 120% the rate of morphine oxidation
286284
Pseudomonas putida M10
codeinone + NAD(P)H
at pH 6.5, + NADPH, reduction of codeinone to codeine
286284
Pseudomonas putida M10
r
dihydrocodeine + NADP+
-
286284
Pseudomonas putida
dihydrocodeinone + NADPH
-
-
-
?
dihydrocodeine + NADP+
-
286284
Pseudomonas putida M10
dihydrocodeinone + NADPH
-
-
-
?
morphine + NADP+
enzyme activity only with NADP+
286284
Pseudomonas putida
morphinone + NADPH + H+
-
-
-
?
morphine + NADP+
enzyme activity only with NADP+
286284
Pseudomonas putida M10
morphinone + NADPH + H+
-
-
-
?
morphine + NADP+
highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source
286284
Pseudomonas putida
morphinone + NADPH
-
-
-
?
morphine + NADP+
highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source
286284
Pseudomonas putida M10
morphinone + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 310000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
enzyme assay
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
in presence of NADPH reduction of codeinone to codeine, in MOPS buffer
Pseudomonas putida
9.5
-
with NADP+ as cofactor, in glycine-NaOH buffer, oxidizing the C-6 hydroxy group of morphine and codeine
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
the Pseudomonas enzyme acts only with NADP+
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
the Pseudomonas enzyme acts only with NADP+
Pseudomonas putida
General Stability (protein specific)
General Stability
Organism
2-mercaptoethanol stabilizes
Pseudomonas putida
dithiothreitol stabilizes
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Pseudomonas putida
2,2'-dipyridyl
-
Pseudomonas putida
CuSO4
-
Pseudomonas putida
iodoacetate
-
Pseudomonas putida
additional information
no inhibition by EDTA, NEM, hydroxyquinoline
Pseudomonas putida
NEM
inhibit activity at much higher concentration (e.g. at 1 mM)
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.044
-
codeine
with NADP+
Pseudomonas putida
0.35
-
NADP+
-
Pseudomonas putida
0.46
-
morphine
cosubstrate NADP+
Pseudomonas putida
2.91
-
Dihydrocodeine
cosubstrate NADP+
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
denatured enzyme, SDS-PAGE
Pseudomonas putida
32000
-
native enzyme, gel filtration
Pseudomonas putida
310000
-
1 * 310000, SDS-PAGE
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
morphine + NADP+
Pseudomonas putida
highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source
morphinone + NADPH
-
-
?
morphine + NADP+
Pseudomonas putida M10
highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source
morphinone + NADPH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
gel filtration, various chromatographic techniques
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.08
-
morphine, NADP+
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, 10% loss of activity over 2 months
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
codeine + NAD(P)+
oxidation at 120% the rate of morphine oxidation
286284
Pseudomonas putida
codeinone + NAD(P)H
at pH 6.5, + NADPH, reduction of codeinone to codeine
286284
Pseudomonas putida
r
codeine + NAD(P)+
oxidation at 120% the rate of morphine oxidation
286284
Pseudomonas putida M10
codeinone + NAD(P)H
at pH 6.5, + NADPH, reduction of codeinone to codeine
286284
Pseudomonas putida M10
r
dihydrocodeine + NADP+
-
286284
Pseudomonas putida
dihydrocodeinone + NADPH
-
-
-
?
dihydrocodeine + NADP+
-
286284
Pseudomonas putida M10
dihydrocodeinone + NADPH
-
-
-
?
morphine + NADP+
enzyme activity only with NADP+
286284
Pseudomonas putida
morphinone + NADPH + H+
-
-
-
?
morphine + NADP+
enzyme activity only with NADP+
286284
Pseudomonas putida M10
morphinone + NADPH + H+
-
-
-
?
morphine + NADP+
highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source
286284
Pseudomonas putida
morphinone + NADPH
-
-
-
?
morphine + NADP+
highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source
286284
Pseudomonas putida M10
morphinone + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 310000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
enzyme assay
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
in presence of NADPH reduction of codeinone to codeine, in MOPS buffer
Pseudomonas putida
9.5
-
with NADP+ as cofactor, in glycine-NaOH buffer, oxidizing the C-6 hydroxy group of morphine and codeine
Pseudomonas putida
Other publictions for EC 1.1.1.218
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
667890
Todaka
Bioactivation of morphine in h ...
Homo sapiens
Biol. Pharm. Bull.
28
1275-1280
2005
-
-
-
-
-
-
14
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
14
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286288
Todaka
Purification and characterizat ...
Mesocricetus auratus
Arch. Biochem. Biophys.
374
189-197
2000
-
-
-
-
-
-
7
3
1
-
2
-
-
1
-
-
1
-
-
1
1
1
3
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
3
1
-
2
-
-
-
-
1
-
1
1
1
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286289
Walker
Mechanistic studies of morphin ...
Pseudomonas putida, Pseudomonas putida M10
Biochem. J.
345
687-692
2000
-
-
1
-
4
-
1
3
-
-
2
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
4
-
-
1
-
3
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286286
Yamano
Purification, characterization ...
Oryctolagus cuniculus
Arch. Biochem. Biophys.
341
81-88
1997
-
-
-
-
-
1
6
9
3
-
3
-
-
2
-
-
1
-
-
2
1
1
9
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
1
-
6
-
9
3
-
3
-
-
-
-
1
-
2
1
1
9
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286287
Yamano
In vivo and in vitro formation ...
Rattus norvegicus, Rattus norvegicus Wistar
Xenobiotica
27
645-656
1997
-
-
-
-
-
-
7
2
1
-
-
-
-
167
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
2
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286285
Walker
Towards engineering an improve ...
Pseudomonas putida, Pseudomonas putida M10
Ann. N. Y. Acad. Sci.
799
6-10
1996
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286284
Bruce
-
Microbial degradation of the m ...
Pseudomonas putida, Pseudomonas putida M10
Biochem. J.
274
875-880
1991
-
-
-
-
-
2
6
4
-
-
3
2
-
5
-
-
1
-
-
-
1
1
8
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
2
-
6
-
4
-
-
3
2
-
-
-
1
-
-
1
1
8
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286283
Bruce
Microbial degradation of the m ...
Pseudomonas putida, Pseudomonas putida M10
Arch. Microbiol.
154
465-470
1990
-
-
-
-
-
1
-
-
-
-
-
4
-
5
-
-
1
-
-
-
1
-
8
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
4
-
-
-
1
-
-
1
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286282
Yamano
Guinea-pig liver morphine 6-de ...
Cavia porcellus, Cavia porcellus Hartley
Biochem. Pharmacol.
35
4321-4326
1986
-
-
-
-
-
-
7
4
1
-
-
-
-
12
-
-
1
-
-
3
2
2
4
-
1
-
-
-
2
-
1
2
-
-
-
-
-
-
2
-
-
-
-
7
-
4
1
-
-
-
-
-
-
1
-
3
2
2
4
-
1
-
-
-
2
-
1
-
-
-
-
-
-
-
286281
Yamano
Purification and characterizat ...
Cavia porcellus, Cavia porcellus Hartley
J. Biol. Chem.
260
5259-5264
1985
1
-
-
-
-
1
3
12
1
-
1
-
-
12
-
-
1
1
-
2
1
1
12
-
1
-
-
-
2
-
2
1
-
-
-
1
-
-
1
-
-
1
-
3
-
12
1
-
1
-
-
-
-
1
-
2
1
1
12
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-